Animal Handling Final

Question 1

Reasons to evaluate blood smears include:

Answer 1

Perform WBC differential, estimate platelet numbers, evaluate cellular morphology, evaluate cellular abnormalities, quality control of analyzing equipment, looking for parasites

Question 1

Reasons to perform cytology include:

Answer 1

Rapid diagnostic information, differentiates neoplasia from inflammation, indicates if further procedures are required

Question 2

What are some ways to collect cytology samples?

Answer 2

Swabs, scrapings, impressions, FNA, biopsy, centesis

Question 3

The order of staining blood smears is as follows:

Answer 3

Fixative, eosin, methylene blue, rinse

Question 4

What angle should we hold the spreader slide when preparing a blood smear?

Answer 4

30 degrees

Question 5

What angle should we hold the spreader slide if preparing a sample of thick blood?

Answer 5

20 degrees

Question 6

What angle should we hold the spreader slide if preparing a sample of thin blood?

Answer 6

45 degrees

Question 7

Which procedure involves obtaining a sample with a dull scalpel blade and placing it on a microscope slide?

Answer 7

Skin scraping

Question 8

True or false: Blood smears need to be dried quickly before staining.

Answer 8

True

Question 9

True or false: We need to use immersion oil to view ectoparasites on a slide.

Answer 9

False

Question 10

What does cytology mean?

Answer 10

The study of cells

Question 11

What technique do we use to collect cells from a mass?

Answer 11

Fine needle aspirate (FNA)

Question 12

True or false: Blood smears need to be evaluated as part of the complete blood count.

Answer 12

True

Question 13

Why should we dry a blood smear immediately after making it?

Answer 13

To prevent dust particles from sticking to it and avoid cell distortion

Question 14

True or false: If understained, cells will appear to be too dark

Answer 14

False

Question 15

True or false: Microfilaria can be picked up on a blood smear

Answer 15

True

Question 16

What are 3 things we need to do with samples that are dropped off and can’t be tested immediately?

Answer 16

Label, put in refrigerator, notify tech/write on white board

Question 17

True or false: Unstained blood smears should be stored in the refrigerator

Answer 17

False

Question 18

If a blood smear is created with the proper technique, what shape would it be?

Answer 18

Thumbprint

Question 19

True or false: Blood smears need to be stained before microscopic evaluation

Answer 19

True

Question 20

True or false: Ear swab samples do not need to be heat fixed before staining

Answer 20

False

Question 21

The 3 regions of a blood smear include:

Answer 21

Body, monolayer (counting area), feathered edge

Question 22

What substance aids in visualization under 1000x magnification?

Answer 22

Immersion oil

Question 23

What are blood tubes used for?

Answer 23

Storing blood for various procedures

Question 24

What are used to facilitate blood and sample collection?

Answer 24

Needles

Question 25

What are cotton-tipped applicators used for?

Answer 25

Ear cleaning and sample collection

Question 26

True or false: When looking for ear mites, samples do not need to be heat fixed or stained

Answer 26

True