Animal Biotechnology - Cloning Flashcards
What is cloning?
Creation of an exact genetic replica of an organism
Can cloning happen naturally?
Yes, through embryo splitting to produce identical twins
What is somatic cell nuclear transfer (SCNT)?
A form of cloning that involves removing the nucleus from a somatic cell and putting it into a donor egg to develop into an embryo
Why was Dolly the sheep such a huge achievement?
She was the first successfully cloned mammal, which is very difficult
Why does SCNT often have problems with the development of the cloned embryos?
Epigenetic reprogramming. Certain genes are silenced and the cloned embryos often have deformities or the pregnancies fail
Can we apply the same techniques used to clone Dolly to other animals?
No, the procedure needs to be optimized for each species. Each procedure will only work for one species
Why is it difficult to do SCNT with extinct species?
SCNT requires fresh, high quality eggs and somatic cells, as well as an appropriate surrogate. With extinct species we don’t have access to fresh cells
How do we optimize the SCNT procedures for each species?
A lot of trial and error for things like hormones and temperature
What are the steps in SCNT?
- Remove the nucleus from a somatic cell
- Transfer that nucleus into a egg cell
- Culture in conditions specific to each species to trigger formation of a healthy embryo
- Transfer embryo into the surrogate
What is the current status of SCNT?
Has now successfully cloned a wide range of organisms, including dogs, pigs, sheep, humans, other primates
What happens when SCNT is done right?
The clone is indistinguishable from a normal organism and is perfectly healthy
How can transgenic animals be created? 3 ways
- Microinjection of DNA into the zygote
- Transformation of embryonic stem cells and microinjection into blastocyst
- Transformation of somatic cells and SCNT
Why are gene-edited animals different from other transgenic animals?
No foreign DNA is introduced, only a targeted change in the genome is made
How can SCNT be applied?
Research, pet cloning, conservation cloning, de-extinction, biofactories, xenotransplantation, invasive species control
Where is the vast majority of animal biotechnology used?
Research, to create and manipulate animal models and cell lines to study development, human diseases, gene function
Are there any SCNT cloned or gene edited animals approved for human consumption?
No
Are there any transgenic animals approved for sale in Canada?
One, the AquAdvantage salmon
How is animal biotechnology applied in agriculture?
Safety, animal well-being, improved agronomics, disease resistance
What are polled cattle?
Cows that have been genetically engineered/gene edited to have no horns, which eliminates the danger to the cows and workers and stops the need for dehorning
How can transgenic animals have improved agronomics?
Converting food to muscle mass more efficiently, produce more milk
How has animal biotechnology been used in agriculture to improve disease resistance?
Transgenic cows that don’t express the protein that can misfold and cause mad cow disease
Why were the Enviropigs created?
Reduce their environmental impact. Pigs are very inefficient at using phosphate in their diets and most of it gets excreted. It is required to add phosphate to their food which costs money and harms the environment