Analytics

Question 1

BGA - Why is it important to perform BGA analysis as soon as possible upon pulling the sample?

Answer 1

To avoid degassing and changes in sample temperature

Question 2

BGA - What does a result reading “—-↑” or “—-↑↑” indicate?

Answer 2

The sample result exceeded the upper range limit for a selected parameter

Question 3

BGA - How often must the Wash/Waste Cartridge be replaced?

Answer 3

Every 10 days

Question 4

BGA - When replacing both the Measurement and Wash/Waste Cartridge, which cartridge
should be replaced first?

Answer 4

Measurement Cartridge

Question 5

BGA - What size syringe is used for BGA sampling analysis?

Answer 5

Up to a 30 mL syringe

Question 6

Osmometer - Why do we test just the supernatant for osmolality and metabolite analysis?

Answer 6

To avoid clogging samples lines of the analytical equipment with cell culture

Question 7

Osmometer - How often should you run the 290 reference standard test?

Answer 7

Once a day

Question 8

Vi-Cell Viability Analyzer - Why is it important to ensure that samples are thoroughly mixed prior to performing Cell
Density and Viability (CDV) analysis?

Answer 8

Cells settle over time; mixing ensures sample homogeneity in order to obtain an accurate cell count

Question 9

Vi-Cell Viability Analyzer - When must you perform manual sample dilution for CDV analysis?

Answer 9

When the total cell density goes above a certain limit (≥ 10 x 106 cells/mL)

Question 10

Vi-Cell Viability Analyzer - How do you log in the samples into the Analyzer prior to testing the sample?

Answer 10

Click on the Log in Sample button

Question 11

Vi-Cell Viability Analyzer - When should the reagent pack be replaced?

Answer 11

When the reagent level meter under the diagnostics menu reads “Runs left: 0”

Question 12

Turbidimeter - What should you be sure to do before inserting the sample into the device?

Answer 12

Gently invert the sample and apply silicone oil to the sample cell with the oil cloth

Question 13

Turbidimeter - What should you do if the 10 NTU fails to pass after a third attempt?

Answer 13

Calibrate the turbidimeter, as described in SOP000501

Question 14

Nova Bioprofile Flex - How long do new auto QC (AQC) Cartridges need to thaw?

Answer 14

At least 30 minutes prior to installation

Question 15

Nova Bioprofile Flex - Why should the sample be spun down in a centrifuge?

Answer 15

To avoid clogging the device with cell culture. Only the supernatant will be used for sample testing

Question 16

Nova Bioprofile Flex - How long should you shake the chemistry cartridge after adding the glutamine cups to the
reagent?

Answer 16

2 minutes to completely dissolve the glutamine powder