Analytical Techniques Flashcards
What does HPLC stand for?
High Performance Liquid Chromatography
How does HPLC work?
- Solvent passes through tube under high pressure.
- Components are detected by absorption of UV.
What are factors that affect retention time?
- Nature of solvent
- Pressure
- Temperature
In GC, what are the stationary and mobile phases?
Stationary = solid or liquid coated on the inside of the tube.
Mobile = inert gas
In GC, what does a greater affinity to the stationary phase mean?
Longer retention time
What are the limitations of HPLC and GC?
If retention time has no reference, data is unhelpful.
What is the main function of each technique in HPLC-MS?
HPLC separates the mixture of substances.
MS identifies the separated substances using relative molecular mass.
How does H NMR spectroscopy work?
- Radio waves interact with the nuclei of hydrogen atoms.
- If nuclei has an odd atomic number or mass number, spin state will be changed and detected.
