Analytical Spec Flashcards
What are the key components of a spectrophotometer?
- Source that generates a broad band of electromagnetic radiation.
- Dispersion device selects broadband radiation.
- Sample Area
- 1+ detectors to measure intensity of radiation
What does it mean if an instrument is double beam?
Light will pass through mirror/lense and split into two beams, giving both a reference and sample. This stops light source drift problem that occurs in single beam
What are the two kinds of light sources in analytical spectrophotometry?
A Tungsten lamp (350-2000nm, suitable for colourimetry) Deuterium lamp (200-370nm, good for UV)
What are the two types of monochromator and what must they contain?
- Prisms
- Diffraction gratings
They must all contain an entrance slit, a collimating mirror (or lens), a dispersing device, a focusing mirror (or lens) and an exit slit. They work by selecting a particular wavelength via Prisms or diffraction gratings.
What is diffraction grating?
It is where radiation enters and the beam is collimated and strikes a dispersing element at an angle, the beam is split into component λ by grating.
What does a detector do and what are the two main types?
Converts a light signal- electrical signal. Photomultiplier tube and photodiode array exist.
Explain how a photomultiplier tube works
Electrons given off, attracted to a dynode, give off more electrodes, reach anode. Amplifies signal.
Explain how a photodiode array works
Liquid passes through, no time to stop, diode array constantly measuring.
What may cause deviations from Beer’s law?
Instrumental: Non-linearity may be caused by stray light in the instrument at high absorbances, causing an underreported value of absorbance.
Chemical: pH- some chromophores shift wavelength with changed pH, this can be overcome using a buffer
Concentration: Above 10mM the close distance between absorbing molecules can alter ability to absorb a given λ
What is a bathochromic (red) shift?
A shift in λ max towards longer wavelength. Usually due to action of an auxochrome (a functional group attached to the chromophore which doesnt absorb light energy itself but influences the wavelength absorbed by chromophore).Auxochromes possess a lone pair of electrons which interact with the Pi electrons of chromophore. Usually associated with decreased light absorption (hypo chromic)
What is a hypsochromic (blue) effect?
A shift in λ max to a shorter wavelength. Occurs when compounds with a BASIC auxochrome ionise and lone pairs can’t interact with electrons of chromophore. Usually associated with increases in light absorption (hyper chromic)
What effect does increasing light source intensity have on absorption?
No effect
What is the difference between a comparative and absolute assay method?
Comparative needs to know concentration.
Absolute must use Beer’s law.
What is fluorimetry?
Analytical technique that relies on the EMISSION of electromagnetic energy by molecules. The light emitted by the sample is always of longer wavelength (lower energy) than the light absorbed by the molecule.
What is internal conversion?
A non-radiative transition between states of the same multiplicity. No light is given off in this process. It is possible to get IC back to ground state but the energy gap is much bigger so less efficient. The loss of energy here accounts for Stokes shift in wavelength from excitation to emission.
What is Inter-system crossing?
ISC is when the spin of an excitated electron is reversed (singlet to triplet transition).
Explain the mirror image rule
The differences between vibrational levels are similar in the ground and excited states so that the fluorescence spectrum resembles the first absorption band.
What is Stokes shift?
The gap between the maximum of the first absorption band and the maximum of fluorescence.