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CLINICAL CHEMISTRY > Analytical methods > Flashcards

Analytical methods Flashcards

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1
Q

<400 nm; short wavelength

A

UV light

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2
Q

> 700 nm; long wavelength

A

Infared light

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3
Q

Number of vibrations of wave motion per second

A

Frequency

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4
Q

Used to check wavelength accuracy

A

Didymium/Holmium oxide filter

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5
Q

Used to verify absorbance accuracy on linearity

A

Neutral density filters & dichromate solution

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6
Q

Adapted for only a limited number of clinical laboratory analysis (lipoprotein particle measurements)

A

Nuclear magnetic resonance (NMR) spectro

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7
Q

Most common light source used in visible & near infrared regions

A

Tungsten light bulb

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8
Q

Light source used in the UV region

A

Deuterium lamp

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9
Q

Continuous source of radiation in both UV & visible region

A

Xenon discharge lamp

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10
Q

Minimizes unwanted/stray light

A

Entrance slit

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11
Q

Line source used in UV & visible regions in spectro

A

Mercury & Sodium vapor lamp

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12
Q

Line source used in UV & visible regions in AAS

A

Hollow Cathode lamp

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13
Q

Most common cause of loss of linearity

A

Stray light

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14
Q

Selects the bandpass

A

Exit slit

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15
Q

Measures light absorbed by atoms in ground state

A

AAS

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16
Q

Measures light emitted by an atom burned in flame
(Excitation of electrons)

A

Flame Emission

17
Q

Measures amount of light blocked
Used for measuring proteins

A

Turbidimetry

18
Q

Unknown sample made to react with known solution

A

Volumetric/Titrimetric

19
Q

Measures the amount of light scattered at an angle of __ used for Ag-Ab complexes

A

Nephelometry ; 15-90°

20
Q

Supporting media for electrophoresis
___ - 5 bands
___ - 10 bands
___ - 15 to 20 bands

A

Cellulose acetate
Agarose
Polyacrylamide Gel

21
Q

Semiquantitative drug screening test

A

TLC

22
Q

Gold standard for drug testing

A

GC-MS

23
Q

Detects 20 inborn error of metabolism

A

MS-MS

24
Q

Used for volatile solutes:
For non-volatile solutes:

A

Gas chromatography
Liquid chromatography

25
Q

Excitation by electromagnetic radiation using mercury/xenon

A

Fluorometry

26
Q

Chemical reaction yields an electronically excited compound that emits light

A

Chemiluminescence

27
Q

Measures differences in voltage potential at a constant current (calomel & silver-silver chloride)
Used for:

A

Potentiometry
pH & pCO2

28
Q

Measures electrolyte dissolved in the liquid phase

A

ISE

29
Q

Electrochemical titration: amount of electricity at a fixed potential
Used for:

A

Coulometry
Chloride test

30
Q

Measures current flow by redox rxn
Used in:

A

Amperometry
pO2, glucose

31
Q

Measures current after potential is applied

A

Voltammetry

CLINICAL CHEMISTRY (1 decks)

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