Analytical methods

Question 1

<400 nm; short wavelength

Answer 1

UV light

Question 2

> 700 nm; long wavelength

Answer 2

Infared light

Question 3

Number of vibrations of wave motion per second

Answer 3

Frequency

Question 4

Used to check wavelength accuracy

Answer 4

Didymium/Holmium oxide filter

Question 5

Used to verify absorbance accuracy on linearity

Answer 5

Neutral density filters & dichromate solution

Question 6

Adapted for only a limited number of clinical laboratory analysis (lipoprotein particle measurements)

Answer 6

Nuclear magnetic resonance (NMR) spectro

Question 7

Most common light source used in visible & near infrared regions

Answer 7

Tungsten light bulb

Question 8

Light source used in the UV region

Answer 8

Deuterium lamp

Question 9

Continuous source of radiation in both UV & visible region

Answer 9

Xenon discharge lamp

Question 10

Minimizes unwanted/stray light

Answer 10

Entrance slit

Question 11

Line source used in UV & visible regions in spectro

Answer 11

Mercury & Sodium vapor lamp

Question 12

Line source used in UV & visible regions in AAS

Answer 12

Hollow Cathode lamp

Question 13

Most common cause of loss of linearity

Answer 13

Stray light

Question 14

Selects the bandpass

Answer 14

Exit slit

Question 15

Measures light absorbed by atoms in ground state

Answer 15

AAS

Question 16

Measures light emitted by an atom burned in flame
(Excitation of electrons)

Answer 16

Flame Emission

Question 17

Measures amount of light blocked
Used for measuring proteins

Answer 17

Turbidimetry

Question 18

Unknown sample made to react with known solution

Answer 18

Volumetric/Titrimetric

Question 19

Measures the amount of light scattered at an angle of __ used for Ag-Ab complexes

Answer 19

Nephelometry ; 15-90°

Question 20

Supporting media for electrophoresis
___ - 5 bands
___ - 10 bands
___ - 15 to 20 bands

Answer 20

Cellulose acetate
Agarose
Polyacrylamide Gel

Question 21

Semiquantitative drug screening test

Answer 21

TLC

Question 22

Gold standard for drug testing

Answer 22

GC-MS

Question 23

Detects 20 inborn error of metabolism

Answer 23

MS-MS

Question 24

Used for volatile solutes:
For non-volatile solutes:

Answer 24

Gas chromatography
Liquid chromatography

Question 25

Excitation by electromagnetic radiation using mercury/xenon

Answer 25

Fluorometry

Question 26

Chemical reaction yields an electronically excited compound that emits light

Answer 26

Chemiluminescence

Question 27

Measures differences in voltage potential at a constant current (calomel & silver-silver chloride)
Used for:

Answer 27

Potentiometry
pH & pCO2

Question 28

Measures electrolyte dissolved in the liquid phase

Answer 28

ISE

Question 29

Electrochemical titration: amount of electricity at a fixed potential
Used for:

Answer 29

Coulometry
Chloride test

Question 30

Measures current flow by redox rxn
Used in:

Answer 30

Amperometry
pO2, glucose

Question 31

Measures current after potential is applied

Answer 31

Voltammetry