Analytical Chemistry

Question 1

Chromatography is the process for identification,
purification and separation of components of a mixture on
the basis of

Answer 1

Difference in their affinity for mobile and stationary
phase

Question 2

Chromatography involves two mutually

Answer 2

Immiscible phases

Question 3

There is no need of support if packed into a column
when the stationary phase is

Answer 3

Solid

Question 4

Which of the following is not stationary phase?

Answer 4

Solid-solid chromatography

Question 5

Liquid chromatography is a technique for separating

Answer 5

Ions that are dissolved in a solvent

Question 6

In exclusion chromatography, solute molecules are
separated based on

Answer 6

Molecular geometry and size

Question 7

The gas-solid chromatography is ______________
chromatography as per basic principle involved.

Answer 7

Adsorption

Question 8

A proper solvent that is passed through the column for
elution so that separated components can be collected is
called

Answer 8

Buffer solution

Question 9

The relative solubility of solute in both the phases
determines the

Answer 9

Rate of movement of solute

Question 10

If the mobile phase is gas, movement of solute is
determined by its

Answer 10

Volatility

Question 11

Column chromatography is based on the principle of

Answer 11

Differential adsorption

Question 12

Arrange the following compounds in order of their
increasing adsorption tendencies.

Answer 12

Cellulose&raquo_space; starch&raquo_space; calcium carbonate&raquo_space; alumina

Question 13

What is the factor responsible for the separation in
column chromatography?

Answer 13

Polarity differences between the solute

Question 14

Select the correct statement from the following options.

Answer 14

The greater the polarity of solute, more strongly it will
be adsorbed on a polar surface

Question 15

The correct order of increasing strength of adsorption is

Answer 15

Alkanes&raquo_space; Esters&raquo_space; Ketones&raquo_space; Aldehydes&raquo_space; Phenol

Question 16

The components of the mixture in column
chromatography are eluted in order of

Answer 16

Increasing polarity and increasing distribution ratio

Question 17

The elution power of a solvent is determined by

Answer 17

All of the mentioned

Question 18

Which of the following is separated through column
chromatography?

Answer 18

Chlorophyll and carotenoids

Question 19

The mixture of petroleum ether and benzene is used in
the elution ratio of

Answer 19

1 : 9

Question 20

Chloroform fraction is eluted from the column by
passing chloroform through the column which acts as

Answer 20

Elution

Question 21

The principle on which thin layer chromatography is
based is that the

Answer 21

Different compounds are adsorbed on an adsorbent to
different degrees

Question 22

The size of a thin layer of adsorbent is about

Answer 22

0.2 mm

Question 23

The chromaplate or thin layer chromatography plate is
made up of

Answer 23

Glass