ANALYTIC TECHNIQUES AND INSTRUMENTATIONS Flashcards
4 basic areas in the analytic techniques and instrumentations
Spectrophotometry
Luminescence
Chromatography
Electroanalytic methods
The sun gets its energy from the process of___
Nuclear fusion
Electromagnetic radiation also known as
Radiant or radiation
Particle of electromagnetic radiation is
Photon
Change particle such as electron in order to move up and down
Electromagnetic waves
Distance between 2 identical points on a consecutive waves
Wavelength
Distance between origin and crest
Amplitude
Number of waves that pass a point per unit time or per person
Frequency
Wavelength x frequency
Speed
Is a wave of alternating electric and magnetic fields
Light
What are 2 categories of light
Visible
Invisible
Infrared, microwaves, radio waves
Invisible
ROYGBIV
Visible
Called as transmitted light
One that our eyes can see
Reflected light
Also called as polychromatic light
Composed of all visible colors
White light
Produces only one color
Monochromatic
350 to 400
Violet
400 to 450
Indigo yellow
450 to 500
Blue orage
500 to 550
Green red
600 to 650
Yellow to indigo
650 to 600
Red to green
Most common light source at visible light near uv and near ir
Incandescent tungsten/ tungsten iodide lamp or halogen quarts lamp
Fluorometry
Mercury hydrogen lamp xenon arc
AAS ( Alomic absorption spectrophotometry
Hallow cathode lamp
@uv range continuous light emission down to 165nm
Deuterium
Uses an electrical rod of rare earth element oxide
Merst glower
Uses silicon carbide heated to 1200 degrees celsius
Globar
Reduces stray light
Prevents scattered light from entering the monochromator
Entrance slit
Device that produces light specific wavelengths
Monochromatic light
Range of wave length transmitted
Efficiency and monochramator
Bandpass
The concentration of a substance directly proportional to the amount of light absorbed and inversely proportional to the logarithm of the light transmitted
Beer lamberts law
The higher the concentration, the higher the absorbed light
Directly proportional
Whenever the concentration is high the transmitted light is lowbats vice versa
Inversely proportional
Measurement of the light transmitted by a solution to determine the concentration of a light absorbing in the solution
Spectrophotometry
Exeferr lamp
Provide electromagnetic radiation
Polychromatic light
Light source
Wedge shaped piece of glass quarts or sodium chloride
Prism
Aluminized surpa
Difraction grating
Types of interference
Constructive and destructive interference
Crest line up together from perfect fit puzzle in resulting to higher amplitude
Constructive interference
Crest and through meet resulting in low amplitude
Destructive interference
Exclusion of unwanted light from entering the monochromator
Entrance slit
Allows only a narrow beam of spectrum to pass through the cuvette
Exit slit
Analytical cell/ sample holder
Cuvette