Analysis of Cell components

Question 1

Optical and Electron microscope

Answer 1

Optical- long wavelength poor resolution. Elecotron- uses electron beam, short wavelength high resolution.

Question 2

Tem and Sem

Answer 2

Tem- Electromagnets to foucus beam. Only through thin specimen, denser section dark as absorb electrons(black and white). High resolution see ultrastructure.
Sem- direct electron beam, knocks of electron gathered in tube to form image. Bulid 3-D by using pattern of electron. Low resolution

Question 3

Cell fractionasion

Answer 3

Cold- reduce enzyme activity, Isotonic- prevent osmosis, Buffered- ph doesn’t fluctuate.
Homogenization- break plasma membrane by blender releasing organelles.
Filteration- to remove large debris and unwamted tissue
Ultracentrifugation- Frament put in centrifuge. Tube low speed heavy organelle go down forming sediment(pellet). Fluid above sediment called supernatant which is removed put in other tube, repeat…