Anaerobes: GNR/GPR/GNC/GPC/Spore/nonspore

Question 1

Which GNR Pits agar?

Answer 1

B. ureolyticus

Question 2

Which GNR has “brick red fluorescence” color?

Answer 2

Provotella & porphyromonas

Question 3

Which GNR grows on BBE?

Answer 3

Bacteroides

Question 4

Which GNR is Kanamycin resistant?

Answer 4

Provotella

Question 5

Which GNR is glucose nonfermenter?

Answer 5

Porphyromonas

Question 6

Which GNR is Vancomycin Resistant?

Answer 6

Fusobacterium, B. Fragilis, Provotella

Question 7

Which bacteria is GNR and forms spindles

Answer 7

F. nucleatum

Question 8

Which GPC forms black colonies?

Answer 8

Peptococcus niger

Question 9

Identification of GNR

Answer 9

Indole
Kanamycin, colistin, vancomycin disks
bile esculin
IKV agar

Question 10

Identification of GPR

Answer 10

Lrg: EYA, reverse cAMP
sml: catalase and indole test

Question 11

Identification of GNC

Answer 11

no further ID

Question 12

Identification of GPC

Answer 12

Disk
Vancomycin and colistin disk
indole test

Question 13

Name 4 GNR

Answer 13

Bacteroides sp
Provotella sp
Porphyromonas sp
Fusobacterium

Question 14

List 7 Bacteroides

Answer 14

B. fragilis (indole-)
B. vulgatus &distasoniis (indole-)
B. thetaiotamicron (indole+)
B. ovatus and uniformis (indole+)
B. ureolyticus- Kanamycin & volistin susceptible, pits agar, requires fummate

Question 15

Bacteroides fragilits

Answer 15

most isolated from humans
- life-threatening dz
- grows on BBE
- with rounded ends w/ vacuoles

Question 16

Provotella & Porphyromonas

Answer 16

-normal microbiota
-oral, GIT, GUT
-PIGMENTED

Question 17

List the 4 Fusobacteriums

Answer 17

F. nucleatum
F. necrophorum
F. mortipherum
F. varium
**group is susceptible to kanamycin & colistin. No growth on KV BAP

Question 18

Fusobacterium nucleatum

Answer 18

speckled opalescent “bread crumb colonies”

Question 19

Fusobacterium necrophorum

Answer 19

irregular margins
-severe form of Vincints Dz

Question 20

Fusobacterium necrophorum

Answer 20

irregular margins
-a severe form of Vincints Dz

Question 21

Fusobacterium mortipherum

Answer 21

fried egg appearance

Question 22

Fusobacterium varium

Answer 22

fried egg appearance
vincents dz
Esculine and lactose fermentation

Question 23

List GPR

Answer 23

Clostridium Botulinum
C. clostridioforme
C. difficile
C. perfringens
C. Tetani

Question 24

C. Botulinum

Answer 24

Four types: food-borne, infant botulism (dust, honey, soil spores), wound botulism (contamination of wounds), undetermined
-blurred VA
-flaccid paralysis (flabby )
-virulence factors: exotoxin that blocks release of acetylcholine
+spores

Question 25

C. Tetani

Answer 25

-lockjaw
-drumstick
-muscular spasm
-tetanospasmin (neurotoxin) inhibits neurotransmitter of motor neurons
-death by respiratory failure

Question 26

C. perfringens

Answer 26

+/- spores
-soft tissue dz: wounds, cellulitis, gas gangrene***
+Nagler (lecithinase)
+reverse cAMP

Question 27

C. Difficile

Answer 27

Antibiotic associated diarrhea
-diag w/ CCFA (cycloserine cefoxitin fructose agar) -yellow green fluorescence, ground-glass
-GDH (glutamate dehydrogenase)
-toxin testing EIA
-PCR for alpha and beta gene
-neut red pH
neutral indicates AA

Question 28

Mobiluncus

Answer 28

strict anaerobe

Question 29

Nonspore forming anaerobes

Answer 29

Propionibacterium
Eubacterium Lentum
Actinomyces israellii
Bifidobacterium dentium

Question 30

Propionibacterium

Answer 30

indole and catalase +

Question 31

Eubacterium lentum

Answer 31

true agent of endocarditis
diphtheroid GPR

Question 32

Actinomyces israellii

Answer 32

endogenous onto damaged tissue
“sulfur granules”
“molar tooth colony”

Question 33

Bifidobacterium dentium

Answer 33

oral (dientes) and GIT
Y or bifid shaped GPR
Inhibits harmful bacteria

Question 34

List 5 GNC (no further testing )

Answer 34

Veillonella- only one in human infections. Normal microbiota
uncommon:
Megasphaera
Anaeroglobus
Negativicoccus
Acidaminococcus

Question 35

List 9 GPC

Answer 35

-Peptostreptococcus-anaerobic- SPS susc
-peptococcus niger- black colony
-Finegoldia-looks like staph
-Staphylococcus saccharolyticus-indole+
-Gallicola
-Parvimonas micros- very small cocci
-Peptinophilus
-murdophilus
-anaerococcus

Question 36

Identifyy organism found as part of normal flora of skin which produces propionic acid by fermentation of glucose

Answer 36

propionibacterium acnes

Question 37

Name an anaerobic genus which may be suspected when the gram stain demonstrates small gram positive cocci chains

Answer 37

peptostreptococcus anaerobus
peptococcus niger
finegoldia
staphylococcus saccharolyticus
parvimonas micros

Question 38

Name the only significant anaerobic GNC

Answer 38

Veillonella spp

Question 39

Describe gram stain morph for bacteroides, fusobacterium, propionibacterium, actinomyces, and clostridium

Answer 39

bacteroides- GNR, safety pin appearance, hydrolyze esculin
Fusobacterium- GNR, slender with pointed ends, “bread crumb”, “speckled” and “smooth”
propionobacterium- nonspore forming B,CB, may branch; diphtheroid
actinomyces israelli-nonspore forming, GPB, branching cells, sulfur granules “molar tooth”
clostridium-GPB, may appear GN,

Question 40

Name 3 organisms associated with gas gangrene

Answer 40

C perfringens- double hemolytic
C sordellii
C histolyticum

Question 41

What organisms is identified with SPS?

Answer 41

peptostreptococcus anaerobius

Question 42

Tests for Bacteroides fragilis

Answer 42

20% bile- safety pin appearance, indole Varies, catalase varies

Question 43

Tests for fusobacterium nucleatum

Answer 43

indole, sensitive to kanamycin, fluroesce chartreuse , three colony appearances: “bread crumb” “smooth” “speckled”

Question 44

Tests for Bacteroides ureolyticus

Answer 44

K and C susceptible and Vancomycin resistent, pits agar, nitrite reduction, urease positive