Anaerobes Flashcards

1
Q

indicator in anaerobe jar

A

impregnated with methylene blue
blue = presence of oxygen
colorless = anaerobic conditions exist

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2
Q

what is PRAS

A

Prereduced anaerobically sterilized

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3
Q

Fusobacterium and C. diff present which color under the UV light?

A

Chartreuse green

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4
Q

Gram stain is positive, but no growth on culture. what could be the reason?

A

patient in antibiotics
wrong plate
no anaerobic condition

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5
Q

Disc pattern for Bacteroides

A

Colistin R
Kanamycin R
Vancomycin R

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6
Q

Disc pattern for Prevotella

A

Colistin S
Kanamycin R
Vancomycin R

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7
Q

Disc pattern for Porphyromonas

A

Colistin R
Kanamycin R
Vancomycin S

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8
Q

Disc pattern for Fusobacterium

A

Colistin S
Kanamycin S
Vancomycin R

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9
Q

Disc pattern for Capnocytophaga

A

Colistin S
Kanamycin S
Vancomycin R

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10
Q

what organisms are positive for Nagler test?

A

C. perfringens
C. sordelli
C. bifermantens
C. barati

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11
Q

Indole is detected by

A

p-dimethylaminocinnamaldehyde to produce blue-green color

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12
Q

what organisms are positive for Indole

A

Cutibacterium acne (P.acne)
Peptostreptococcus asaccharolyticus

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13
Q

what organism gives false negative indole?

A

Clostridiums

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14
Q

gram stain, disc pattern, and nitrate for Veillonella

A

GNC
Colistin S
Kanamycin S
Vancomycin R
Nitrate +

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15
Q

Usual disc pattern for GP organisms

A

Colistin R
Vancomycin S

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16
Q

Disc pattern for P. asaccharolyticus

A

Colistin R
Kanamycin S
Vancomycin S

17
Q

Disc pattern for P. anaerobius

A

Colistin R
Kanamycin R
Vancomycin S

18
Q

Media used for C. diff

A

CCFA
Cycloserine-Cefoxitin Fructose Agar

19
Q

What are the aerotolerant organisms

A

C. histolyticum
C. teritum
P. acne

20
Q

What organism has sulfur granules and have molar tooth colony morphology?

A

Actinomyces

21
Q

What is GNC anaerobes

A

Veillonella

22
Q

Microaerophilic anaerobe GNR?

A

Capnocytophaga

23
Q

dog bone or forked end morphology

A

Bifidobacterium

24
Q

which organism causes pseudomembranous colitis and antibiotic-associated diarrhea

A

C. diff

24
Q

What are anaerobe GPC

A

Peptostreptococcus

25
Q

GPR
chain
catalase -

A

Lactobacillus

26
Q

GPR
curved
aerotolerant
catalase +
indole +

A

P. acne

27
Q

Swarmer Clostridiums

A

C. septicum
C. tetani

28
Q

GPR
tennis racket shapes
potent neurotoxins (neuroparalytic disease)

A

C. botulinum

29
Q

Double zone hemolysis
alpha exotoxin
gas gangrene
motility -

A

C. perfringens

30
Q

what organisms gives brick red fluorescence under the UV light

A

Prevotella
Porphyromonas
Veillonella

31
Q

Lactobacillus resembles

A

C. tertium

32
Q

Fusobacterium clinical disease

A

necrotizing faciitis

33
Q

potent neurotoxin (neuroparalytic disease)
non-acidic vegetables, mushrooms, canned food, honey

A

C. botulinum

34
Q

GNR anaerobes
Growth on BBE/LKV (black pigment on BBE)
Disc pattern RRR
causes pelvic inflammation

A

Bacteroides fragilis

35
Q

which media does Mycoplasma pneumoniae grow, gives what morphology, and clinical disease?

A

PPLO (pleural pneumonia-like organism) agar
spherical, grainy
Walking pneumonia

36
Q

which media does Mycoplasma hominis grow, gives what morphology, and inhabits?

A

A8 media
Fried egg morphology
inhabits the vagina, but can cause urethritis

37
Q

Which media does Ureaplasma urealyticum grow, gives what morphology, and found in?

A

A8 media
Very tiny colonies
normal flora in vagina, but can cause urethritis and infertility

38
Q

what organism causes trisma?

A

Lock jaw
C. tetani