ANACHEM LEC Flashcards
branch of chemistry that is involved with the analysis of chemical substances
ANALYTICAL CHEMISTRY
measurement science consisting of a set of powerful ideas and methods that are useful in all fields of science and medicine
ANALYTICAL CHEMISTRY
Used to detect the presence in a sample, “what is present?’
Qualitative Analysis
Used to measure how much of that substance is present in the sample
Quantitative Analysis
Also known as wet chemical methods, earliest method of analysis, relied mainly on the chemical properties of analytes
CLASSICAL METHODS
Titration
CLASSICAL METHOD
THESE ARE EXAMPLES OF WHAT ANALYTICAL METHOD
- Separation of analytes by precipitation, extraction, or distillation
- Qualitative analysis by reaction of analytes with reagents that yielded products that could be recognized by their colors, boiling or melting
points, solubilities, optical activities, or refractive indexes - Quantitative analysis by gravimetric or titrimetric techniques
CLASSICAL METHODS
USE OF A CERTAIN DEVICE, INSTRUMENT, OR MACHINE
INSTRUMENTAL METHODS
RELY ON THE MEASUREMENT OF PHYSICAL PROPERTIES
INSTRUMENTAL METHODS
LIGHT ABSORPTION: HOW MUCH LIGHT YOUR SAMPLE CAN ABSORB
SPECTROPHOTOMETER
FLUORESCENCE: HOW MUCH YOUR SAMPLE CAN EMIT LIGHT
FLUOROMETER
THIN LAYER, PAPER, AND COLUMN CHROMATOGRAPHY
CLASSICAL METHODS
HIGH PERFORMANCE LIQUID CHROMATOGRAPHY, GAS CHROMATOGRAPHY
INSTRUMENTAL METHODS
USED CATHOD AND ANODES TO ANALYNZE DNA
SDS PAGE
BEER LAMBERT’S LAW
HIGH CONCENTRATION = HIGH ABSOBENT
DETERMINE THE MASS OF THE ANALYTE OR SOME COMPOUND CHEMICALLY REALATED
GRAVIMETRIC METHODS
KNOWN AS VOLUMETRIC METHOD, DETERMINES THE VOLUME
TITRIMETRIC METHODS