ANA LE 1 Flashcards
What is the main function of epithelial cells?
Lining of surface or body cavities; glandular cavities
4 basic types of tissues
Epithelium, muscle, nervous and connective
Functions of epithelium
Barrier & Protection
○ E.g. skin, oral mucosa
● Absorption
○ E.g. small intestine absorbs nutrients and water
● Secretion
○ E.g. digestive enzymes (intestinal epithelium), mucus (respiratory
epithelium)
● Lubrication
○ E.g. mesothelium (outermost covering of abdominal organs) secretes
small amount of tissue fluids
● Reproduction
○ E.g. linings of male and female reproductive tracts
● Transport
○ E.g. allows small molecules & ions to pass through the epithelium on either direction
Characteristics of epithelial cells
● Avascular
● Exhibits polarity
● Presence of basement membrane or basal lamina
● Presence of junctional complexes or cell junctions
Avascular
○ No blood vessels
○ Nutrients and oxygen are acquired via diffusion from its neighboring
blood vessels
What are the 3 poles of epithelial cell?
○ Apical pole: opposite a space (usually a lumen)
○ Basal pole: domain in contact with ECM & connective tissue
○ Lateral surface: pole in contact with adjacent cells
Functions of Basement Membrane
→ Filter (e.g. renal glomeruli)
→ Provide structural support for epithelial cells
→ Attach epithelium to underlying connective tissue
→Important in maintaining cellular functions (endocytosis, signal
transduction, cell-to-cell interactions, polarity, etc.)
→Epithelial repair and regeneration (e.g. stem cells); acts as the
scaffolding to rebuild on after injury
2 Components of Basal Membrane
→ Basal lamina: Electron-dense, closest to the basal portion of the cell
→ Reticular lamina: More diffuse, fibrous
What is the intermediate filament of epithelium?
Keratin
Junctional complexes for cell-to-cell adhesions
Occluding/Tight Junctions - Zonula Occulens
Anchoring Junctions - Zonula Adherens
Communications / Gap Junctions or Nexus
Cell-to-Cell Extracellular Matric Junctions
Hemidemosomes
What is/are the transmembrane proteins involved in Zonula Occulens?
Claudin & Occludin
What is/are the transmembrane proteins involved in Zonula Adherins?
Cadherin & Cadenin
What is/are the transmembrane proteins involved in Demosomes?
Desmoglein & Desmocollin
What is/are the transmembrane proteins involved in Gap Junctions/Nexus?
Connexin
What is/are the transmembrane proteins involved in Hemidemosomes?
Integrins
What is the purpose of Zonula Occulens and where is it found?
(Zonula: encircles the entire cell)
● Found at the most apical portion –> This would ensure that all molecules
enter or exit the cell via transcellular pathway (vs. paracellular pathway)
Transcellular vs Intracellular
○ Transcellular: it will go into the cell
○ Paracellular: in between cells
What is the purpose of Zonula Adherins and where is it found?
● Like a belt around the cell (it surrounds the cell)
● Immediately below tight junctions
● Anchors cells closely to neighboring cells
What is cellular junction that is calcium requiring and what is the transmembrane that binds with the other cell?
Zonula Adherens and Cadherins
What are the 2 Anchoring Junctions?
Zonula Adherens and Macula Adherens
What is the purpose of Demosomes and where is it found?
○ Does not form a belt around the cell but rather it appears like a
“spot-weld”
○ Provides stability to cells especially those under shearing stress to keep
neighboring cells intact
What is Pemphigus Vulgaris and what is the cellular junction associated with it?
Faulty desmosome function is correlated with various blistering skin
conditions
What are the opening in gap junctions called and what are their functions?
Connexons
Allows small molecules and nutrients selectively between cells (<1.5
nanometers)
What are the function of Gap junctions and where can they be found?
● Allows cells to function as a unit or together
● Organs that need gap junctions:
○ Heart muscles - required to contract as a single unit (many gap junctions)
○ Abdominal/Visceral organs - requires to function rhythmically
What does a hemidemosome resemble?
Demosome
What are the function of Integrins?
→ Forms crosslinks
→ Reacts with laminin molecules (located at the basal lamina)
What is Epidermolysis Bullosa and what is the cellular junction associated with it?
Faulty hemidesmosomes -> blistering condition (Epidermolysis Bullosa)
→Rare condition which causes to have a blister even with minimal friction
What are the types of cell-to-cell extracellular matrix junctions and where are they located?
Hemidemosomes and focal adhesion
Located in the basal membrane
What is the purpose of focal adhesions and where is it found?
○ Resembles hemidesmosomes
○ More numerous and smaller
○ Anchors cells at its basal portion
What are the characteristics of cilia?
○ Long (longer and larger than microvilli) ○ Highly motile ○ Axoneme is the core protein structure ○ Uniform in height ○ Attached to apical part of the cell
What is the core protein structure of Cilia?
Axoneme
Does Cilia have a specific arrangement? What is it’s arrangement when a cross section is done in the middle and on the basal area?
● Arranged in a specific arrangement
If cross section is done through the middle portion:
○ Doublet: 2 microtubules
○ 9 + 2 assembly of microtubules:
→Centrally= PairedMicrotubules(2)
→ Peripherally = (9) Microtubule Doublets
➢ Called an Axoneme
● If cross section is done through the basal portion:
○ Structurally similar to centrioles
○ 9 + 0 assembly of microtubules
○ (9) microtubule triplets without axoneme
○ 0Triplets are linked together in a pin-wheel arrangement with NO central microtubule
What is the function of Cilia and where can it be found?
● Function: “To propel”
○ RespiratoryTract:
→ Sweep or move fluid, cells, particulate matter across cell surfaces
→ Rid air passages of particulate matter or mucus
○ Oviduct:
→ Moves egg cells and sperm cells along the passageway
○ Testes:
→ Move mature sperm into the epididymis
What are the type of cilia that are abundant in columnar and cuboidal cells in epithelia?
Motile Cilia
What are the types of Cilia?
Three types of cilia:
○ Motile cilia: Lines adult human body (e.g respiratory system)
○ Non-motile/primary: No 2 central doublets; lines sensory organs (e.g
Organ of Corti: Inner ear)
○ Nodal cilia: Found in developing embryo (surrounding the primitive node)
that helps in the proper orientation of developing cells during gastrulation
What are the characteristics of microvilli?
Characteristics: ○ Microscopic finger-like projections ○ Uniform in length ○ Shorter and smaller than cilia ○ Non motile ○ Core of thin actin microfilaments → The vertical actin microfilaments attached to actin microfilaments at the cytoplasm called “Terminal Webs” ○ Dirty looking appearance “Striated” (e.g Proximal Convolut
What is the intermediate filament of microvilli?
keratin
What is the vertical actin microfilaments attached to actin microfilaments at the cytoplasm called?
“Terminal Webs”
What is the function of microvilli and where can it be found?
Functions: Absorption; Increase surface area of the cell
○ Intestinal Lining
→ Increase surface area for absorption
→ Densely packed so they are visible as brush/striated border
○ Proximal convoluted tubule
→ Reabsorption of substances such as glucose and amino acids
What is another name for microvilli?
Striated border: Rigid similar in thickness, height, and width (seen in
intestines)
○ Brush border: Different height, width, and thickness (seen in PCT of
kidney)
What are the characteristics of stereocillia?
● Characteristics:
○ Non motile
○ Related to microvilli (both non motile), not cilia (motile) ○ Longer and more slender than microvilli
○ Distal portion is usually branched
What is the function and location of stereocillia?
● Function:
○ Concentrates contents of an organ of lumen
○ Facilitates absorption; increases surface area of cells ○ Inner ear sensory cells
● Location:
○ Epididymis: For absorbing fluid
○ Inner ear: Function as mechanoreceptors
What are the characteristics of flagella?
Characteristics:
○ Whip-like motile structures
○ Made up of microtubules; related to the sperm cells ○ Function: Motility
What are the characteristics , function, and location of simple squamous epithelium?
● Thin, single layer of flattened cells
○ Nucleus is also flattened
○ “Squama” = Latin word which means Scale; which resemble a fish scale
(flat) ● Function:
○ Peritoneal Cavity: Reduces friction between visceral organs by producing lubricating fluid & transport fluids
○ Cardiovascular system: Allows passive transport of fluids, nutrients, and metabolites across the thin capillary walls
○ Alveolar lining of lungs: Facilitates diffusion of gasses and small molecules; more efficient gas exchange
○ Cornea: Lining of the inner surface
○ Loop of Henle: Outer covering (thin loops) ● Typically specialized:
○ Lining of vessels and cavities where they regulate passage of substances into the underlying tissues
○ Can be called specific names depending on location:
→Mesothelium: Serous lining of of pericardium (heart), and peritoneal
cavities
→ Endothelium: Lining of blood vessels, capillaries and lymphatics → Function: Diffusion
Mesothelium
Mesothelium: Serous lining of of pericardium (heart), and peritoneal
cavities
Endothelium
→ Endothelium: Lining of blood vessels, capillaries and lymphatics
What are the characteristics , function, and location of simple cuboidal epithelium?
● One layer of cells that are as long as they are wide (cube/round)
● Greater thickness allows cytoplasm to be rich in mitochondria and other
organelles for a high level of transport across the epithelium
● Function:
○ Ovary: Lining and protection
○ Pancreatic Ducts: Transport
○ Proximal Convoluted Tubule: Absorption of filtered substances ○ Thyroid: Activate secretion of substances into the filtrate
○ They usually form ducts
● Locations:
○ Renal connecting tubules
○ Periphery of the thyroid follicle
○ Germinal epithelium (covering of ovary)
What are the characteristics , function, and location of simple columnar epithelium?
● One layer of cells that are taller than they are wide
● They usually have apical surface modifications: Cilia or Microvilli
● Columnar cells are specialized in absorption
● Seen in lining in stomach (no goblet cells), intestine (with goblet cells) and
gallbladder (digestive organs), renal collecting duct, oviducts (fallopian
tubes) and ductuli efferentes testis
● Can often be termed as “terminal bars”
● Functions:
○ Absorption ○ Protection ○ Lubrication ○ Secretion
What are the characteristics , function, and location of pseudostratisfied columnar epithelium?
● One layer of tall cells
● All the tall columnar cells rest on the basement membrane, but not all cells
are of the same height; creates illusion of stratification
● Stratified appearance is due to the nuclei of the cells placed in different
levels and not all cells extend to the free surface
● Functions:
○ Secretion
○ Protection
○ Cilia-mediated transport
● Locations:
○ Lining of the upper respiratory tract (trachea, bronchi, bronchioles) ○ Lumina of the epididymis and vas deferens
What are the characteristics , function, and location of STRATIFIED SQUAMOUS NONKERATINIZED EPITHELIUM?
● Non-keratinized or Wet type
● Function: Protection
● Location: Areas subjected to constant friction
○ Esophagus ○ Oral Mucosa ○ Vagina
○ Cervix
○ Anal Canal ○ Cornea
● Multiple layers of cells, cuboidal at the base, polyhedral at the middle or columnar, and flattened cells at the top with no keratinization
What are the characteristics , function, and location of STRATIFIED SQUAMOUS KERATINIZED EPITHELIUM?
● DryType
● Thick skin (Palms and soles)
○ Stratum corneum is very thick ○ Topmost layer is flattened
○ More keratinized layers, dense
● Thin skin
○ With hair follicles
○ Stratum corneum is thinner
○ Lesser keratinization/Fewer cell layers
● Lack of nuclei in the topmost layer (Thin lamellae, dead skin cells)
● Function: Protection and prevention of water loss
● As the cells mature from the basal area, they go up the surface, lose their
organelles, and become keratinized
What are the characteristics , function, and location of STRATIFIED CUBOIDAL EPITHELIUM?
● ● ● Not common Multiple layers of cells that are tall as they are wide Functions: ○ Protection ○ To produce secretions Location: ○ Ducts of sweat glands ○ Developing ovarian follicles ○ Large excretory ducts
What are the characteristics , function, and location of STRATIFIED COLUMNAR EPITHELIUM?
● Quite rare ● Location:
○ Conjunctiva
○ Largest Ducts of Exocrine Glands ○ Anorectal Junction
What are the characteristics , function, and location of TRANSITIONAL EPITHELIUM?
● Found only in mammals ● Location: Urinary Passages ○ Bladder ○ Ureters ○ Renal Calyces ● If relaxed, ○ Have features that are intermediate between stratified cuboidal and stratified squamous ○ Topmost layer: Dome-shaped ● If stretched, ○ Topmost layer will be flattened ● Has plaques ○ Serve as an osmotic barrier (Not uniform throughout) ○ Prohibit passage of water and salts
What are the types of glands based on path of release?
Endocrine, Paracrine and Exocrine glands
Where do Endocrine glands originate and what are their products of secretion?
○ Originates from epithelial cells that lost connection to surface
epithelium and ducts
○ Products of secretion → target organ via the bloodstream
○ Example: Pituitary Gland, Thyroid Gland, Pancreas
Where do Paracrine glands originate and what are their products of secretion?
○ Products → target surrounding cells
○ Example: Cells of the pancreas when it release somatostatin that target
other pancreatic cells; growth hormone producing cells during early development
Where do Exocrine glands originate and what are their products of secretion?
○ Originates from the epithelial cells that maintained connection to
surface epithelium
○ Products → target organ via the ducts
○ Example: Sweat Gland, Salivary Gland, Pancreas
Unicellular vs multicellular glands. Which one is the most common one?
● Unicellular Glands
○ Glands composed of a single cell (e.g. Goblet cells in large intestine)
○ Flask-shaped and nucleus is found in the narrow basal part of the cell;
apical part has mucin filled vesicles
Multicellular glands
○ The most common type of gland; composed of multiple cells
Serous vs mucous glands
● Serous Glands
○ Secretory portion are composed of serous cells
○ Serous cells form spherical mass of cells with a lumen in the center
called acini that produces serous fluid
○ Secretions are thin and aqueous and contain enzymes
○ Description: pyramidal with a broad base, rounded nucleus, granular and
basophilic cytoplasm due to apical portion containing secretory vesicles and basal portion containing rough endoplasmic reticulum
● Mucous Glands
○ Secretory portion are composed of mucous cells
○ Mucous cells form tubules that produces mucin or mucous
○ Secretions are viscous and made up of mucin and water
○ Description: cuboidal or columnar, its nuclei is flattened towards the
base, sometimes nuclei appear elongated due to mucous accumulation
pressing it towards the base
○ Is lightly stained due to mucous getting dissolved in alcohol
What surrounds the serous and mucous cells - and initial portions of the ducts, and helps propel the secretion to the exterior via the intercalated duct?
Myoepithelial cells
What is the initial duct, what is it made of and where are the secretions of this brought to?
Intercalated duct which is made of simple cuboidal epithelium and its secretion is brought to the striated ducts
The large presence of striations at the basal layer of the cell brought about the infoldings of the plasma membrane, together with the elongated mitochondria, interspersed between the infolded membrane
Striated Duct
Serous and mucous secretions brought to the exterior via the ___ will be then led to the ___. from there, the products of the gland are now brought to the ___, then to the ___ (ducts in between the lobes).
Intercalated duct, striated duct, intralobular duct, interlobular duct
Types of glands and their difference
Simple Glands (do not branch; 1 duct + secretory portion/s) and Compound Glands (several branched secretory portions)
What are simple tubular glands?
elongated secretory portion; ducts are short/absent
mucus glands (colon and intestinal glands)
What are branched tubular ducts?
several long secretory portions
glands in the uterus and stomach
What are simple coiled tubular glandsWhat are simple coiled tubular glands
long coiled secretory potions
sweat glands
What are simple acinar (alveolar) glands?
rounded, sac-like secretory portions
small mucous glands (urethra)
What are simple branched acinar glands
multiple sac-like secretory portions
sebbaceous glands
What are compound tubular glands?
several elongated, coiled secretory portions - ducts converging into a larger duct
submucosal brunner glands (duodenum)
What are compound acinar glands?
Several sac-like secretory portions - small ducts converging into a larger duct
exocrine pancreas
What are compound tubulacinar glands?
a combination of tubular and acinar secretory portions converging into a larger duct
salivary glands
What are the types of glands based on the mechanism of product release?
Merocrine (Eccrine) gland, Holocrine gland, and Apocrine gland
Merocrine (Eccrine) vs Holocrine vs Apocrine gland. Which is the most common one?
● Merocrine (Eccrine) gland
○ The most common type of glands (based on gland-cell participation)
○ Secretions are released or brought to the duct via exocytosis (through
the secretory vesicles)
○ E.g., salivary glands
Holocrine gland ○ Undergoes apoptosis (i.e. cell death) ○ Secretory portions become part of the secretion (sacrificed and formed part of the secretion) ○ E.g., sebaceous glands
Apocrine gland
○ Part of the apical surface of the cell is pinched or released with the
secretion of the gland
○ E.g., Mammary gland
Cells renew continuously via
Mitosis and stem cell populations
Rate of renewal of small intestine and epidermis
4 days intestine
28 days epidermis
- Which of the following cellular features is used in naming types of
epithelia?
a) Shape of cells in the basal layer
b) Number of cell layers
c) Presence of a basal lamina
d) Size of the nuclei
e) Nature of the cell junctions that are present
B
- Exocrine glands in which the acini all produce a secretion of heavily glycosylated, hydrophilic proteins are an example of which type of gland? a) Serous gland
b) Mixed gland
c) Mucous gland
d) Tubuloacinar gland e) Simple gland
D
- Which of the following is found during meiosis but not mitosis?
a) Chromatids
b) Polar microtubules
c) Metaphase
d) Synapsis
e) Cytokinesis
D
- A mixed salivary gland is classified morphologically as a compound tubuloacinar gland.
a) True
b) False
A
- Cilia are long, motile, structures with a core of 9 microtubule triplets arranged around a pair of microtubules.
a) True
b) False
B
Refer to the glands and epithelium lecture trans for the pictures
6) Cilia - blue arrow; Basement membrane - yellow arrow. Note: not Basal lamina since one can only see it using an electron microscope
7) Both are simple squamous epithelium (blue and black arrow)
8) Pseudostratified ciliated columnar epithelium with goblet cells
9) Transitional epithelium (relaxed; but no need to mention)
10) Stratified squamous epithelium non keratinizing/wet type
11) Stratified squamous epithelium keratinized/dry type (Thin skin)
12) Simple cuboidal epithelium with microvilli/brush border/striated border
What is the function of peroxisome?
Detoxify specific harmful substances either produced by the cell or taken into the cell, engage in beta oxidation of fatty acid from Acetyl CoA
Basic structural and functional unit of the human body
Cell
Study of cells
Cytology
Study of tissues
Histology
Study of Organs
Organology
What parts of the cell can be seen via Light Microscope?
Cytoplasm, Nucleus and Nucleolus
What parts of the cell can be seen via Electron Microscope?
Cell membrane, Centriole, Chromatin, golgi apparatus, mitochondria, nucleus, nucleolus, nuclear envelope, nuclear pore, endoplasmic reticulum
What should be observed when examining a cell?
Shape and size of cell. shape and size of nucleus, if nucleolus is present
- Which of the following is an intermediate filament protein found in cytoplasm of most filament cells?
a. Actin
b. Vimentin
c. Myosin
d. Laminin
e. Keratin
E
- Which of the following types of epithelium withstands the hypertonic effect of fluids?
a. Simple Cuboidal
b. Simple Squamous
c. Stratified Columnar
d. Transitional
D
- Functions of the basement membrane include which of the following?
a. Contractility
b. Molecular filtering
c. Active ion transport
d. Excitability
e. Modification of secreted proteins
B
- Functions of the basement membrane include which of the following?
a. Contractility
b. Molecular filtering
c. Active ion transport
d. Excitability
e. Modification of secreted proteins
B
- The release of lipid droplets from cells is which type of secretion?
a. Merocrine
b. Serous
c. Apocrine
d. Mucous
e. Holocrine
C
- TRUE or FALSE? The basal lamina is a thin sheet of macromolecules that can be appreciated using light microscopy.
False
- TRUE or FALSE? The zonula adherens are disc-shaped cell junctions that provide stability to cells subject to shearing and stresses.
False
- TRUE or FALSE? Microvilli are long, motile, structures with a core of uniformly arranged microtubules.
False
Supine
Recumbent, lying on the back facing upwards