ANA LE 1 Flashcards
What is the main function of epithelial cells?
Lining of surface or body cavities; glandular cavities
4 basic types of tissues
Epithelium, muscle, nervous and connective
Functions of epithelium
Barrier & Protection
○ E.g. skin, oral mucosa
● Absorption
○ E.g. small intestine absorbs nutrients and water
● Secretion
○ E.g. digestive enzymes (intestinal epithelium), mucus (respiratory
epithelium)
● Lubrication
○ E.g. mesothelium (outermost covering of abdominal organs) secretes
small amount of tissue fluids
● Reproduction
○ E.g. linings of male and female reproductive tracts
● Transport
○ E.g. allows small molecules & ions to pass through the epithelium on either direction
Characteristics of epithelial cells
● Avascular
● Exhibits polarity
● Presence of basement membrane or basal lamina
● Presence of junctional complexes or cell junctions
Avascular
○ No blood vessels
○ Nutrients and oxygen are acquired via diffusion from its neighboring
blood vessels
What are the 3 poles of epithelial cell?
○ Apical pole: opposite a space (usually a lumen)
○ Basal pole: domain in contact with ECM & connective tissue
○ Lateral surface: pole in contact with adjacent cells
Functions of Basement Membrane
→ Filter (e.g. renal glomeruli)
→ Provide structural support for epithelial cells
→ Attach epithelium to underlying connective tissue
→Important in maintaining cellular functions (endocytosis, signal
transduction, cell-to-cell interactions, polarity, etc.)
→Epithelial repair and regeneration (e.g. stem cells); acts as the
scaffolding to rebuild on after injury
2 Components of Basal Membrane
→ Basal lamina: Electron-dense, closest to the basal portion of the cell
→ Reticular lamina: More diffuse, fibrous
What is the intermediate filament of epithelium?
Keratin
Junctional complexes for cell-to-cell adhesions
Occluding/Tight Junctions - Zonula Occulens
Anchoring Junctions - Zonula Adherens
Communications / Gap Junctions or Nexus
Cell-to-Cell Extracellular Matric Junctions
Hemidemosomes
What is/are the transmembrane proteins involved in Zonula Occulens?
Claudin & Occludin
What is/are the transmembrane proteins involved in Zonula Adherins?
Cadherin & Cadenin
What is/are the transmembrane proteins involved in Demosomes?
Desmoglein & Desmocollin
What is/are the transmembrane proteins involved in Gap Junctions/Nexus?
Connexin
What is/are the transmembrane proteins involved in Hemidemosomes?
Integrins
What is the purpose of Zonula Occulens and where is it found?
(Zonula: encircles the entire cell)
● Found at the most apical portion –> This would ensure that all molecules
enter or exit the cell via transcellular pathway (vs. paracellular pathway)
Transcellular vs Intracellular
○ Transcellular: it will go into the cell
○ Paracellular: in between cells
What is the purpose of Zonula Adherins and where is it found?
● Like a belt around the cell (it surrounds the cell)
● Immediately below tight junctions
● Anchors cells closely to neighboring cells
What is cellular junction that is calcium requiring and what is the transmembrane that binds with the other cell?
Zonula Adherens and Cadherins
What are the 2 Anchoring Junctions?
Zonula Adherens and Macula Adherens
What is the purpose of Demosomes and where is it found?
○ Does not form a belt around the cell but rather it appears like a
“spot-weld”
○ Provides stability to cells especially those under shearing stress to keep
neighboring cells intact
What is Pemphigus Vulgaris and what is the cellular junction associated with it?
Faulty desmosome function is correlated with various blistering skin
conditions
What are the opening in gap junctions called and what are their functions?
Connexons
Allows small molecules and nutrients selectively between cells (<1.5
nanometers)