AMYLASE - ELECTROLYTES

Question 1

Catalyzes the breakdown of starch and glycogen via a,1-6 branching linkages

Answer 1

Amylase

Question 2

Tissue source for amylase

Answer 2

Acinar cells of pancreas and Salivary gland

Question 3

measures the appearance of starch citrate

Answer 3

Amyloclast

Question 4

measures the appearance of the product from the starch

Answer 4

Saccharogenic

Question 5

measure the increase in color

Answer 5

Chromogenic

Question 6

Coupling of several enzyme systems to monitor amylase activity

Answer 6

Continuous monitoring

Question 7

Reference range of amylase in:
Serum -
Urine -

Answer 7

25-130 u/L
1-15 u/hr

Question 8

Salivary Amilase

Answer 8

ptyalin (fast moving)

Question 9

Pancreatic amylase

Answer 9

Amylopsin (slow moving)

Question 10

Smallest enzyme

Answer 10

Amylase

Question 11

Normal range for Amylase

Answer 11

60-180 u/L

Question 12

Tissue source in Lipase

Answer 12

pancreas, stomach and intestine

Question 13

Hydrolyzes of fats to produce alcohols and FA

Answer 13

Lipase

Question 14

indicator of Cherry crandall

Answer 14

Phenolphthalein

Question 15

Thymolpthalein + Veronal indicator

Answer 15

Tietz

Question 16

Substrate for enzyme (both cherry crandall & tietz)

Answer 16

50 % olive oil (triolein)

Question 17

Breakdown of Lipids primarily Triglycerides

Answer 17

Lipase

Question 18

Enzymes that the tissue factor are heart, liver, skeletal muscle, kidney, erythrocytes

Answer 18

Lactate Dehydrogenase

Question 19

Forward Reaction: Lactate - pyruvate

Answer 19

Wacker Method

Question 20

Reverse Reaction: Pyruvate - Lactate

Answer 20

Wrobleuski la due

Question 21

Optimal pH of wacker method

Answer 21

8.3 - 8.9

Question 22

Optimal pH of Wrobleuski la due

Answer 22

7.1-7.4

Question 23

reference range of Lactate Dehydrogenase

Answer 23

100-225 u/L(37 deg. C)

Question 24

Catalyzes interconversion of lactic & pyruvic acids using coenzyme NAD, in either forward or reverse direction.

Answer 24

Lactate Dehydrogenase

Question 25

Enzymes that is the tissue sources are skeletal muscle, heart muscle, brain tissue

Answer 25

Creatine kinase

Question 26

Molecular weight of Creatine kinase

Answer 26

82,000

Question 27

Forward Reaction of creatine kinase.optimum pH: 9.0

Answer 27

Tanzer-Gilbarg

Question 28

Reverse Reaction of creatine kinase. Optimum pH: 6.8

Answer 28

Oliver-Rosalki

Question 29

Reference range of CK

Answer 29

Male: 15-160 u/L Female: 15-130 u/L

Question 30

Associated with ATP regeneration in contract, e or transport systems

Answer 30

Creatine Kinase

Question 31

Absorbance of p-Nitroaniline

Answer 31

405-420 nm

Question 32

Ions capable of carrying an electric charge.

Answer 32

Electrolytes

Question 33

Anion

Answer 33

Negative charge

Question 34

Positive charge which moves towards CATHODE

Answer 34

CATIONS

Question 35

major extracellular cation

Answer 35

Sodium

Question 36

Fluids inside the cell and accounts for about 2/3 of total body water.

Answer 36

Intracellular Fluid

Question 37

Any fluid not contained in the cell. Found in the vessels. Accounts for 1/3 of total body water

Answer 37

Extracellular fluid

Question 38

The portion of total body warer contained within the epithelial-lined spaces

Answer 38

Transcellular fluid

Question 39

Also known as natrium

Answer 39

Sodium

Question 40

Hypernatremia

Answer 40

Serum Na is >150 mEq/L

Question 41

Hyponatremia

Answer 41

Serum Na is <125 mEq/L

Question 42

Major intracellular cation

Answer 42

Potassium

Question 43

Major extracellular anion

Answer 43

Chlorode

Question 44

Normal reference range of sodium

Answer 44

135-145 mEq/L