AMT Parasitology Flashcards
Pathogenic, 4 nuceli, central karyosome, cigar shaped chromatid, progressive motility
E.histo
The only Ameba that ingests rbcs and is diagnositic for
E.histo
Infective, nonmotile, nonfeeding stage of E.histo
cyst
Identical to E.histo, non patho, doen’t ingest
E.dispar
Nonpatho, up to 8 irregular nuclei, eccentric karyosome, pointed chromatid, sluggish motility
E.coli
Similiar to E.histo, smaller and nonpatho
E.hartmanni
Nonpatho, glycogen vacuole, blot karyosome, no peripheral chromatin
Only ameba with one nucleus in cyst
Iodamoeba butshlii
nonpatho, smallest ameba, 4 nuclei, blot karyosome, no peripheral chromatin
E.nana
Two free living amebae
Naegleria fowleri
Acanthamoeba spp
Cause of PAM (meningoencephalitis), nasal passage
Naegleria fowleri
Diagnostic stage of N.fowleri
trophs in CSF
Ameba in immuncompromised, keratitis, contact lens
Acanthamoba spp
Vacuolar, granular forms
with 4 nuclei pushed to side,
large center
no cyst
Blastocystis
6 Flagellates
Dientamoeba fragilis (no flagella though)
Giarlia lamblia
Chilomastix mesnili
Trichomonas spp
trypanosome, leishmania
Two flagellates with no cyst stage
D.fragilis
T.hominis
Flagellate with no visible flagella, no cyst, 2 nuclei (each a mass of granules) and pathogenic
Dientamoeba fragilis
Cause of gasteroenteritis, beaver fever, cyst infective
axostyle, parabasal body, falling leaf motility, old man, sucker on underside, day care
Giardia lamblia
Nonpatho flagellate
lemon shaped cyst
Spiral grove/cork skrew motility, jaws
Chilomastix
mesnili
Flagellate, pathogenic, undulating membrane is 1/2 the length
sexually transmitted, urogenital
T.vaginalis
Flagellate nonpatho, feces, undulating membrane full length, no cyst
T.hominis
Only ciliate pathogenic to humans
Balantidium coli
pathogenic ciliate
farmers,pigs
cilia, 2 nuclei (one kidney) macro/micro
Balantidium coli
Infective stage of Plasmodiium and vector
sporozoites by female Anopheles mosquito
Stain for plasmodium
Giesma
Resistant to P.vivax
Duffy neg
These two things resistant to P.falciparum
G6PD def
HmgS
Exoerythrocytic phase of plasmodium in what organ
liver
Most infections of babesia by what species
microti
6 sporozoa
Cryptosporidium, Cyclospora, Isospora
Babesia, Plasmodium
Toxoplasma
Infective stage of Babesia and what vector
sporozoites
bite of deer tick
(Ixodes scapularis)
Ring forms in packets of four, maltese cross from what protozoa
Babesia
Diagnostic stage in Giesma for Babesia
Ring forms in packets of four, maltese cross
P.ovale and P. vivax have these 3 things in common
rbcs enlarged
young rbcs
schuffners dots
Maurers dots seen in which Plasmodium
P.Falciparum
Ziemans stippling seen in which Plasmodium
P.malariae
Crescent or banana shaped gametocytes seen in
P.falciparum
Name of hemoflagellates living in human blood and tissues
trypanosomes
Stage with no flagella, leishmanial form
amastigote
stage with flagella at poterior end
trypomastigote
Two species cause of African sleeping sicknesss
Trypanosoma
rhodesiense, gambiense
East sleeping sickness
rhodesiense
West sleeping sickness
gambiense
Infective stage and vector of trypanosoma
trypomastigote, TseTse fly
Diagnostic stage in Giesma of trypanosoma
trypomastigote
Trypanosoma cruzi
disease name, infective, and vector
Chagas
tyrpomastigote
feces of reduvid bug
(T.infestans)
Diagnostic stage of T.cruzi in Giesma
tryptomastigote
What two forms and where can be found to diagnosis T.cruzi
Amastigote in heart
Trypomastigote in blood
Trypanosome that can be spread thru transfusions and seen in heart muscle
T.cruzi
Obligate intracellular parasite, flagellate
kala azar
amastigote in WBCs, RE cells
Leishmania
Leishmania infective stage, vector and diagnostic
promatigotes
sandfly
diagnostic: amastigote in macrophage
Genus with amastigote in macrophage in Giesma from skin lesions
(LD bodies)
Leishmania
3 parasites spread thru transfusions
Trypanosoma (chagas)
Plasmodium
Babesia
Diagnostic and infective stage for intestinal cocidia/sporozoan
oocysts
Coccidia/Sporozoan transmitted via fecal, self limiting, AIDs, acid fast oocyts
Cryptosporidium parvum
Intestinal coccidia/sporozoan with infective oocysts, contaminated produce/summer
Cyclospora cayetanensis
Sporozoan where human is definitive host, anorexia, diarrhea
oocysts/sporoblasts in feces
Isospora belli
Obligate intracellular, opportunisitics/AIDS
ingest spores w/polar tubule
Microsporidia
Diagnosis of microsporidia
trichrome
Tissue cocidia cat feces/undercooked meat, death of fetus, AIDs
oocyts
Toxoplasma gondii
4 parasites spread thru blood
Plasmodium
Babesia
Leishmania (macrophages)
Trypanosome(plasma)
Cause of pneumocystis pneumonia (PCP) immunocompromised
Pneumocystis jiroveci, carinii
Stain for P.jiroveci/carinii
GM silver stain
Gomoris methenamine silver stain
Another name for Flukes
Trematodes
5 trematodes/flukes
CFF, SP
Clonorchis
Fasciola
Fasciolopsis
Schistosoma
Paragonimus
Giant intestinal fluke
Fasciolopsis buski
Chinese liver fluke
Clonorchis sinensis
Sheep liver fluke
Fasciola hepatica
1 Blood Fluke
3 species
Shistosoma
japonicum, mansoni
haematobium
1 Lung Fluke
Paragonimus westermani
Cestodes are
Tapeworms
3 intestinal tapeworms/cestodes
1 tissue
D.latum
Taenia
Hymenolepsis
E.granulosus
Larval form in tissue Cestode/tapeworm
Echinococcus granulosus
How many specimens recommended, frequency and over how many days for parasites
3 specimens every other day, over 10 days
How soon need to examine liquid specimens if not in preservative
30min of passage
How soon to examine soft specimens if not in preservative
1hr
How soon to examine formed stool if not in preservative or refridgerated
24hrs
Do not collect specimens that contain these 3 things and why
urine: destroy motile
water: free living
oil/barium enema:
protozoa may become undetectable
Collection of specimens in general
Sputum: early morning
CSF: don’t dilute
Urine: 1st void
Genital: saline wet swab
Tissue/skin: sterile
Blood: Thick concentration, thin for id
Most common stain and gold standard used for pernmanet smear
trichrome
Trichrome shows…and is what color
background
shows nuclear chromatin
is red-purple
background is blue green
2 stains that also can be used for blood smears
Wright, Giesma