AMT MICRO Flashcards
1
Q
TSA w/sheep blood, diff w/hemolysis, most nonfastidious bacteria
A
SBA Sheep blood agar
2
Q
Isolation of Haemophilus and Neisseria, factors X &V, incubated at increased CO2
But best for…
Neisseria gonnerhea better on…
A
CHOC, chocolate agar
Better for Haemophilus
Best for gonnerhea and meningitis is modified thayer martin
3
Q
CHOC supplies which factors and is incubated with increased what?
A
X, V, increased CO2
4
Q
colistin-nalidixic acid agar, CNA
isolates…
suppresses…
A
isolates GP
suppresses GN
5
Q
PEA
isolates for … and …
inhibits
A
isolates GPC and anaerobic GNR
inhibits enteric GNR
6
Q
PEA
A
Phenyl ethyl alcohol agar
7
Q
CNA
A
colistin-nalidixic acid agar
8
Q
EMB
A
eosin methylene blue
9
Q
EMB,
isolates…
inhibits….
LF are … To …
E.coli what kind of color…
A
isolates enteric GNR
inhibits GP
LF are green-black to purple
E.coli have a green metallic sheen
10
Q
MAC
isolates…
inhibits…
LF color…
A
isolates enteric GNR
inhibits GP
11
Q
bile salts and crystal violet that inhibit most GP are in what medium
A
MAC
12
Q
SMAC
sorbitol MacConkey isolates…
…doesn’t ferment sorbitol and are colorless
A
E.coli 0157:H7
13
Q
4 medias for isolation of salmonella and shigella
A
SS, HE, XLD, GN
14
Q
Hektoen enteric (HE)
isolates these two
inhibits
A
Salmonella and shigella
inhibits normal GI flora
15
Q
XLD
A
xylose lysine deoxycholate
16
Q
Cold loving, optimum temp 15C
A
psychrophiles
17
Q
moderate temp, 37C most pathogenic organisms
A
mesophiles
18
Q
heat loving, 50-60C
A
thermophiles
19
Q
obligate anaerobes are harmed by
A
O2
20
Q
Facultative anaerobes can grow…
A
with or w/out O2
21
Q
microaerophiles prefer
A
lower O2
22
Q
capnophiles prefer
A
higher CO2
23
Q
Aerotolerant
A
do not need O2, and not hurt by it
24
Q
Typical incubation CO2%
temp/name of ideal temp
humidity
A
5-10% CO2
35-37C/98.6F/optiumum
50-70% humidity
25
Autoclave lbs, mins, temp
15lbs, 15min, 121C
26
QC for wet sterilization/autoclave
Bacillus
stearothermophilus
27
Two halogens that are disinfectants
idoine and chlorine
28
Process that inactivates or inhibits microbes (not spores)
and example
disinfection
Bleach (1:10)
29
Process that kills all microbes including spores
and examples
sterilization
autoclaves
30
Penicillin
beta lactam
inhibits cell wells
31
Gram neg anaerobe resistant to peniciilin
B.fragilis
32
Two antibiotics best indicators for poor disk storage
penicilin and methicillin
33
Vancomycin is drug of choice for these two microbes
C.difficile and MRSA
34
Vancomycin is a
glycopoeptide
35
Kirby-Bauer Method uses what agar
MH
Mueller-Hinton
36
McFarland is what concentration and how many organisms
0.5
10*8
37
MIC
minimum inhibitory concentration
lowest concentration that prevents growth
first dilution w/no growth
38
MBC
minimum bacteristatic concentration
lowest concentration results in >99.9% killing
39
E-test
MIC on a stick
40
Modified Thayer-Martin good for these two
N.gonorrhea and N.meningitis
41
Thayer Martin media is what kind of agar
enriched chocolate
42
Difference between TM and Modified and what does modified inhibit
MTM has trimethroprim lactate which inhibits swarming of Proteus
43
Middlebrook 7H10 and Lowenstein-Jenson good for
Which one best for observing microscopically
Mycobacterium
Middlebrook 7H11
44
Species for QC of dry heat oven
B.subtilis
45
BBE
good for
B.fragilis
Bacteroides Bile Esculin
46
CCFA
Cycloserine-Cefoxitin
for
C.difficile
47
Regan-Lowe and
Bordet-Gengou
for...
which one preferred...
B.pertussis
Regan-Lowe preferred
48
BCYE
buffered charcoal yeast extract for
Legionella
49
Cystine-Tellurite, Tinsdale and Loefflers Medium
C.diphtheriae
50
Never refridgerate these 3
CSF, anaerobic and GC
51
Blood culture preped with...
draw before...
dilution/ml
prep with alcohol, iodine
draw before fever spike
1:10 dilution, 10mL
52
2 Stains for acid fast/mycobacteria
best of the two...
Kinyoun and Ziehl-Nielson
Kinyoun
53
Calcofluor white stains...
KOH is used to...
stains yeast/fungi
KOH breaks down debris and mucous
54
Gram stain
primary stain, decolorizer, mordant and counterstain
Crystal violet
acohol/acetone
iodine
safranin
55
JEMBEC plates used to
transport and grow N.gonorrhoeae
56
Modified Thayer-Martin, Martin Lewis
NYC, GC-LECT,
JEMBEC isolate
N.gonorrhoeae and meningitidis
57
3 species of enterobacteriaceae that are nonmotile
shigella and klebsiella
Yesenia at 35C
58
Species of enterobacteriaceae that is both motile and nonmotile at diff temps
Yersinia motile at 25/RT, not at 35
59
Two genuses that form spore
Bacillus and clostridium
60
Disinfectant for walls/floors that doesn't kill spores/myco/noneveloped
quarternary ammoniums
61
Phenol
disinfectant, doesn't kill spores
62
2% aqueous glutaraldehyde
used when items can't be sterlized by heat
cold sterlization
63
Sodium polyanethod sulfonate (SPS)
used to grow bacteria by preventing clotting, phagocytosis and bactericial effects
64
Urethral contamination of urine is greather than Squamous epis/hpf
10
65
Resistance to penicillin should be tested for
beta lactamase production
66
beta-lactamase test that uses nitrocefin and there is hydrolysis of the beta lactim ring resulting in red color
Chromogenic cephalosporin metod
or Nitrocefin test
67
Tests beta lactamase w/penicillin-pheno red substrate leading to color change from red to yellow to penicilloic acid
Acidimetric method
68
Test for beta lactamase where betalactamse hydroyze penicillin G and reduce iodine, resulting in fading of blue to colorless
Iodometric method
69
Potassium tellurite used to id
Corynebacterium diphtheriae
70
Potassium tellurite inhibits...
color of C.diph
gp/gn nornal flora
gray black
71
Loeffler serum agar enhances these two things for C.diph
pleomorphism and metachromatic granules
72
Elek test detects toxin for
C.diph
73
TCBS
thiosulfate citrate bile salts
used for
Vibrio
74
2 kinds of alpha heme
s.pneumo
viridians
75
3 kinds of beta heme
Group A, B
L.mono
76
1 gamma heme
enterococci
77
GPC resistant to bacitracin
Staph
78
Cause of toxic shock and food poisoning
S.aureus
79
Staph that is nonheme and common cause of hospital aquired UTI
S.epi
80
staph that is mostly novobiocin sensitive
CNS
81
Antibiotic disk that diff between CNS and S.sapro
S.Sapro versus CNS susceptibility
Novobiocin
All CNS suspectible to Novobiocin
except S.sapro is resistant
82
Strain of CNS that is resistant to novobiocin
S.sapro
83
Pigmented, high domed large GPC
micrococcus
84
Test that diff staph versus strep
catalase
85
Mannitol salt agar, MSA selects for
S.aureus, pos
86
90% of strep, cause of rheumatic fever, scarlet fever
Group A Strep
S.pyogenes
87
Strep that is sensitive to Bacitracin
How many units on disks
Group A strep
S.pyogenes
0.02-0.04
88
Strep NF of female genital and cause of neonatal issues
Group B strep
S.agalactiaae
89
Diff Group A from B with what antibiotic...
which one is which
Bacitracin
Group A is sensitive
Group B is resistant
90
Strep that is hippurate poss and CAMP pos (shows arrowhead)
group B strep
S.agalactiae
91
beta Strep that is PYR pos
Grp A strep
92
Non heme strep that causes nosocomial UTI and hydrolyzes esculin
Group D Strep
nonenterococci
93
two examples of Group D strep
S.bovis, S.gallolyticus
94
Used to be called strep, alpha/non heme, hydrolyzes esculin and grows in NaCL
enteroccouccus
95
Two oval GPC organisms that can cause nosocomial UTI, is in GI, and hydrolyze esculin
Group D and enterocccocci
96
What diff Group D and enterococci
Enterococcus grows in NaCl
97
GPC lancet shaped, alpha heme, encapsulated normal in uppper respiratory
S.pneumo
98
Strep that is sensitive to Optochin
S.pneumo
99
P disk is called
Optochin
100
Strep that is sensitive to optochin and is bile soluble
S.pneumo
101
2 Tests that diff S.pneumo from viridians
Which one is pos for both
optochin and bile solubility
S.pneumo pos
viridians neg
102
Group F
microaerophillic
S.milleri
103
GPC that has butterscotch odor
microaerophillic
s.milleri
104
Large GPC that are nonpatho, in environment/skin, pigmented and microdase pos
micrococcus
105
Antibiotic that will kill MRSA
Vancomycin
106
Two genera with GN dipplococci and oxidase pos
Neisseria, Moraxella
107
GNDC coffee bean shaped, oxidase pos,
Neisseria
108
GNDC that ferments glucose only
N.gonn
109
GNDC that is uppe respir, ferments both glucose and maltose
N.meningitidis
110
GNDC of upper respiratory in kids, ferments all glucose, maltose and lactose
N.lactamica
111
Organism that causes upper respiratory, otitis media, hockey puck colonies
M.cattarhalis
112
GNDC that is neg for sugars, DNase pos, butyrate esterase pos
M.catarrhalis
113
2 oxidase pos GNDC genera
N.mening,gonn
M.catt
114
GNDC needs Co2, 48hrs to grow, ferments glucose only
N.gonn
115
N.mening transmitted by...
seen mostly in...
ferments these two sugars...
respiratory drops
kids
glucose, maltose
116
These two test diff M.cat from Neisseria
DNase pos
butyrate esterase pos
117
M.cat seen in...
resp infections
118
spore forming GP rods
bacillus
119
4 nonspore forming GP rods aerobic
CLEN
Corynebacterium
Listeria
Erysipelothrix
Nocardia
120
CLEN acronym for nonspore forming GP rods aerobic
Corynebacterium
Listeria
Erysipelothrix
Nocardia
121
Gram variable/pos with clue cells, bacterial vaginosis
Gardnerella vaginalis
122
GPR that appears as chinese letters and has metachromatic granules
Corynebacerium
diphtheriae
123
Which medium will help enhance metachromic granules in C.diphtheriae
Loefflers
124
Which GPR has metachromatic granules on Loefflers
C. diphtheriae
125
Elek test tests for...
in what organism
toxin production
C.diphtheriae
126
Ingrient in Tinsdale agar and color change due to its hydrolysis
organism diff on this
tellurite
black
C.diphtheriae
127
GPR with tumbling motility/umbrella motility at RT
L.monocytogenes
128
GPR with test tube brush growth in gelatin and cause of occupational infections for fisherman etc
Erysipelothrix
rhuiopathiae
129
GPR spores, ground glass
Bacillus
130
Bamboo shoots, medusa head
B.anthracis
131
Bacillus that causes food poisoning due to fried rice toxin
B.cereus
132
Most Bacillus are motile except for
B.anthracis
133
GPR on skin/mucous, club shaped
Corynebacterium
134
GPR grows on Loeffler, has metachromic granules, has TOX gene
Corynebacterium
diphtheriae
135
GPR that is hospital acquired, most commonly isolated diphtheroid
C.jeikeium
136
GPR that can cause issues in newborns and food poisoning
L.mono
137
GPR causing skin/lung infections and has sulfur granules
Nocardia
138
Nocardia that causes skin infectionsq
N.brasiliensis
139
Nocardia that causes lung infections
N.asteroides
140
What2 things diff L.mono fron diptheroids/corynebacterium
motility, salicin
141
Another name for metachromatic granules
Babes-Ernst granules
142
Potassium tellurite inhibits...
normal flora so that C..diph can grow
143
GP to Gvariable cocco bacillius that can be grown in cold enrichment
L.mono
144
Diptheroid that is NF in throat
C.pseudo-diphtheriticum
145
Two tests to diff L.mono from Corynebacterium
which one pos/neg
motility and salicin
L.mono: motile, salicin +
Cory: nonmotile, salicin -
146
What stain does mycobacteria use
acid fast, Kinyoun
147
NALC and NaOH do what for mycobacteria
NALC liquefy
NaOH digest/decontaminate
148
Mycobacteria need to be incubated with...at...
with 5% CO2, at 35C
149
Two mediums for mycobacteria, which one clear and can examine microscopically
Middlebrook 7H11: clear plate
Lowenstein-Jensen: green slant
150
Tween 80 test diff and is pos in
pos turns...
neg is...
Myco kansasii
pink/red
amber
151
What can distinguish M.tb from others
Nitrate and niacin
152
Niacin and nitrate can differentiate these two mycobacteria
M.tb
M.bovis
153
M.tb is nicacin/nitate...
M.bovis is niacin/nitrate...
tb is pos
bovis is neg
154
Arylsulfatase test diff what genus
Arylsulfatase is an enzyme that splits free phenolphthalein from the tripotassium salt of phenolphthalein disulfite. The test for the identification of .... species is performed in a tube containing a substrate of phenolphthalein.
mycobacterium
155
3 stains for acid fast
Kinyoun
Ziehl-Neelsen
Fluorochrome
156
Primary stain, decolorizer, and counterstain in Kinyoun and Ziehl-Neelsen stains
Primary: carbolfuchsin
Decolorizer: acid alcohol
Counter: Methylene blue
157
Primary stain, decolorizer, and counterstain in Fluorochrome stain for acid fast
Primary: Auramine-rhodamine
decolorizer: acid alcohol
Counter: potassium permanganate
158
Color of AFB on Kinyoun and Ziehl-Neelsen
red rods, blue backgroud
159
Which acid fast stain requires heat
Ziehl-Neelson
160
Which acid fast stain requires cold
Kinyoun
161
Diagnostic test and source for M.leprae
acid fast tissue
162
Hansens disease is also called
Leprosy
163
Macrophages w/acid fast bacilli
lepra cells
164
Lumpy jaw cause
A.israelii
165
Actinomyces israeli is ana or aero
anaerobic
166
GNR not NF, swimmers ear, Motile, grape like odor
Oxidase pos, cat pos
Pseudomonas aeruginosa
167
GNR bacterial diarrhea, seagulls, darting corkskrew motility
Campybacter
jejuni
168
GNR ulcers, urea breath test
Helicobacter pylori
169
GNR TCBS, rice water stools, motile, comma shaped
Vibrio cholerae
170
GNCB upper respiratory, CHOC, can have capsules
H.influenze
171
Haemophilus pink eye
aegyptius
172
Haemophilus STD,chancroid, bipolar, schools of fish
ducreyi
173
Haemophilus that require both X and V
influenze, aegyptius
174
Haemophilus that requires only X (hemin)
ducreyi
175
H.parahaemolyticus and parainfluenzae requires which factor
V
176
GNR cause of trench fever, cat scratch disease
Bartonella
177
GNCB obligate intracellular parasite
inhalation; with undulant fever, unpasterized milk, animals, bioterrism
Brucella
178
GNCB tularemia/rabbit fever, level 3 pathogen
Francisella
179
GNR from air conditioners, Pontiact fever, BCYE (needs cytsteine)
Legionella
180
GNCB Pasterurella that is caused by bite/scratch, musty odor
multocida
181
Legionella that is brown on Feeley-Gorman iron cysteine agar
L.pneumophila
182
String test, positive result looks like
If the result is positive, the bacterial cells will be lysed by the sodium deoxycholate or Sodium taurocholate, the suspension will lose turbidity, and DNA will be released from the lysed cells causing the mixture to become viscous. A mucoid “string” is formed when an inoculating loop is drawn slowly away from the suspension.
183
String test diff these three genuses and also diff these two species
Vibrio spp. from Aeromonas spp. and Plesiomonas shigelloides.
Vibrio cholerae (string test positive) from other Vibrio spp. (string test negative).
184
Vibrio that is yellow on TCBS, string test pos
Vibrio cholerae
185
Vibrios that are green on TCBS and cause bacteremia
parahemolyticus, vulnificus
186
The only Brucella that doesn't grow on basic fuchin, grows on thionin
suis (pigs)
187
The only Brucella that needs CO2, also thionin neg
abortus (cow)
188
The only Brucella that doesn't produce H2S
melentensis (goat)
189
Bordetella that is urease positive, oxidase positive
bronchiseptica
190
GN bipolar, plague, fleas/rats, safety pin
Yersenia pestis
191
HACEK
all HACEK members are fastidious Gram-negative bacteria associated with infective endocarditis.
Most HACEK are capnophilic, mouth flora, pathogenic in immunocompromised
Haemophilus
Actinobacillus/Aggregatibacter
Cardiobacterium hominis
Eikenella corodens
Kingella kingae
192
Campylobacter is best in what atmospheric condition
obligate microaerophillic
likes chickens, 42C
193
obligate microaerophillic
curved GN rod
gull winged
likes monstly N
Campy
194
Campy gas mixture %
O, CO2, N
85% N2
10% CO2
5% O2
195
curved GNR nonferment, microaerophillic, nitrate pos
C.jejuni
196
How to stain myco-plasma and urea-plasma
Dienes stain
Mycoplasma colonies can be identified by observing typical fried egg colonies on culture medium. Visualization is facilitated by application of Diene’s stain directly to the agar surface. Diene’s stain is a nonspecific stain that imparts a contrasting appearance to Mycoplasma colonies on agar. It allows easy visualization of colony morphology and characteristics.
197
Two genus of bacteria that are the smallest free living and have no cell wall
Myco-plasma and Urea-plasma
198
Cause of primary atypical pneumonia, walking pneumonia
M.pneumoniae
199
Which free living microbe has fried egg appearance
M.pneumoniea
200
How to stain and how to visiualize T.pallidum
silver
darkfield
201
Cause of syphilis
Treponoma pallidum
202
How is leptospira passed to humans
via animal urine
203
Borrelia that causes relapsing fever
B.reccurentis
204
Cause of Lyme disease
transmitted by/vector
B.burgdorferi
Ixodes ticks
205
Chlamydiaceae, Rickettsia, Coxiella, and Ehrlichia all are
obligate intracellular parasites
206
Chlamydiaceae have no...in their cell wall
and are stained with...
peptidoglycan
Giesma
207
Chlamydia species STD
trachomatis
208
Chlamydia with parrot fever
psittaci
209
Chlamydia ass. w/Guillian Barre and grown on HELA cells
pneumoniae
210
Test that uses Proteus OX antigens to detech rickettsial antibody
Weil Felix
211
5 Rickettsiae conditions
Rickettsial pox
Rocky Mt. Spotted Fever
Q and murine fever
Typhus fever
212
Q Fever is actually caused by this rickettsiael organism
Coxiella burnetti
213
PRAS
pre reduced anaerobically sterilized
media
214
Classic principle of anaerobic culture
Jar tech, palladium pellets
H2/CO2 w/water
indicators
215
GPR with double zone of beta, box car, gas gangrene, food
Clostridium perfringens
216
Clostridium that doesn't usually have spores
C.perfringens
217
Naegler test on egg yolk agar used for
C.perfringens
pos/has lecithinase activity
218
Molar tooth, lumpy jaw GPR anarobe
Actinomyces israelii
219
Acceptable Anaerobic specimens
BF, Blood, Bile, BM
asipirate, wound
Biopsy, tissue
220
Unacceptable anaerobic specimens
swabs
sputum, washings, gastric
voided, catheterized urine
feces
221
Anaerobe that forms black, dark halos on BBE due to esculin hydrolysis
Bacteroides fragilis
222
Ideal atmosphere for anaerobes
80-90% N
10% CO2
5%H
223
QC for anaerobes
O2 is color, white colorless no O2
224
1 GPC anaerobe
1GNC anaerobe
GPC: Pep-
GNC: Veillonella
225
Finegoldia magna, Peptostreptococcus, Peptoniphilus all are what kind of microbes
anaerobic GPC
226
4 GPR anaerobic
Clostridium
Lactobacillus
Actinomyces
Propionibacterium
227
Anaerobic GPR with molar tooth, affects brain/facw
Actinomyces
228
2 clostridium that have oval subterminal spores, 1 that may have
botulinum
difficile
(perfringens may hay subterminal)
229
2 Clostridium with round terminal spore
tetani, ramosum
230
Anaerobic GPR antibiotic ass. diarrhea
C.difficile
231
Anaerobic GPR NF skin, acne
Propioni-bacterium
232
Anaerobic GPR NF mouth/female genital, lactic acid/pH
Lactobacillus
233
Anaerobic GNC
NF upper, red flouresnce
Veillonella
234
Anaerobic GNR lung infection, tapered, fluoresence chartreuse/green yellow
Fusobacterium
235
Anaerobic GNR head,neck flurousence brick red
Prevotella
236
4 nonmotile
Shigella, Klebsiella (Enterobacteria)
B.antracis (rest bacillus are motile)
Yesenia nonmotile at 35
237
Tuft of flagela at polar end
Lophitrichous
238
Uniform flagella over body
Petitrichous
239
Amphitrichous
Tuft of flagella at both poles
240
5 Have capsules:
Most are respiratory
Crypto neo
Kleb pneumo
S.pneumo
H.influ
N.memin
241
3 stains for metachromatic granules, best one
MAN
Methylene blue best
Albert
Neisser
242
Forms of sterilization
Autoclave 121, 15, 15
Filtration
Boiling
Fractionalization
Dry heat
Ethylene oxide gas
243
Gram stain ingredients
Crystal violet
Iodine
Ethyl alcohol
Safranin
244
Ziel/kinyoun stain ingredients
Carbol fushin/phenol
HCl/alcohol
Methylene blue
245
Naso swabs used for these two
H.influ
N.mening
246
Urine culture
Process time...
Loop size, multiply by...
Sig CFU...
1hr, 24hr fridge
0.001ml, x1000
>100,000 sig
247
Sputum stain, best sample req
Gram
<10 epi, >25 PMN/lpf
248
Chromagar
Oxacillin, Cefoxitin used for
MRSA
249
Diff microccous from Strep
Catalase
Micrococcus cat pos
250
Staph epi from s.sapro
Novobiocin susceptible
251
Bacitracin susceptible
PYR pos
GAS
252
Oxiase positive PVNM-CH
Pseudomonas
Vibrio ch
Neisseria
M.catt
Campy
Heli,Haemo
253
Genital culture
Transport with these 2, At
Culture w/in, on
Transport modified Stuart's or Amies charcoal at RT
Culture with in 12hrs on JEMBEC
254
Agar melts at, solidify at
Melts 100
Solidify 45-50
255
Four ingredients in modified TM
VCN, TL
Vancomycin
Colistin
Nystatin
Trimethoprim Lactate
256
Nitrocefin is a...
Used to detect...
Turns..
Chromogenic cephalosporin
Detect cleavage of beta lactam ring
Red
257
X factor
V factor
X Hemin
V NAD
258
Needs X,V
satelitte phenom
kids
meningitis,epiglotitis
H.influe
259
Diff L.mono from GrpB strep
L.mono is motile and catalase pos
260
Two microbes that are CAMP and hippyrate pos
GrpB
L.mono
261
Salicin used to diff these two.. which one pos
L.mono from Corynebacterium
L.mono salicin pos
262
GPR partially acid fast, beaded, branching, filamentous. Urease positive
Nocardia
263
Curved Gram negative rod, sea-gull winged
Microaerophillic
Needs increased Nitrogen
42C
Campylobacter
264
Campy req
Atmosphere, temp
Microaerophillic
85 N
10 Co2
5 O
42C
265
Aspects of enteriobacteriace
GNR
Oxidase negative
Catalase pos
Nitrate to nitrite
Motile except for kleb,shig,yer
Ferments glucose, acid
266
Selective media for S&S with bromthymol blue and acid fuchsin as indicators
HE
267
GPR, spores, nonmotile
String of pearls w/penicillin, Medusa
B.anthracis
268
Urease +
PUNCH
Proteus
Urea-plasma
Nocardia
Crytoneo,Corynebac
Helipylori
269
Media where Mercury droplets can be seen in B.pertussis
Bordet gengou
270
E.Coli IMViC
++--
271
Kleb IMViC
--++
272
Most common cause of UTI females
Ecoli
273
Selects with Crystal violet, bile salts....diff with lactose
MAC
274
Selects with eosin Y, methylene blue...diff w/sucrose and lactose
EMB
275
Selects with bile salts, diff lactose/sucrose/salicin and has H2S indicator
HE
276
What are clear and colorless on HE? What is clear with black dot?
Shigella clear
salmonella clear w/black dot
277
What color are NF from the intestine on HE
orange/pink
278
What is the H2S indicator in He, SS, and XLD
Sodium thiosulfate
not the same one in TSI agar (ferric ammonium sulfate/citrate)
279
selects with brilliant green, biles salts and diff with lactose and H2S
SS
280
Selects witih bile salts, diff with lactose/surcrose/xylose and H2S
XLD
281
Two enterobact that produce H2S
Salmonella and proteus
282
Enterobactero DNase pos and red
Serratia
283
Deaminate...
Enzymes that catalyse this reaction are called...
deaminases
Deamination is the removal of an amino group from a molecule.
Deamination occurs in the presence of the deaminase enzyme that catalyzes the breaking of the bond that binds the amino group to the rest of the amino acid.
284
Deaminase/Rapid urease pos: acronym
Deaminase/Urease: PPM
Proteus
Providencia
Morganella
285
PPM are all pos/have these two enzymes
Proteus
Providencia
Moroganella
deaminase, urease
286
Typhoid fever caused by
Salmonella typhi
287
Positive nitrate test is...
confirm with...
Pink
zinc powder
if the medium turns red after the addition of the nitrate reagents, it is considered a positive result for nitrate reduction because it was reduced to nitrite.
If it's clear either nitrate is present or it reduced past nitrite.
Confirm with zinc.
After zinc if it turns red, it's a true negative, nitrate didn't reduce.
If it's clear after zinc, then nitrite was reduced further and is positive.
288
TSI yellow butt
glucose
289
Yellow slant
lactose or sucrose
290
A/A which sugars
slant: lactose and/or sucrose
butt: glucose
291
K/A
butt: glucose only
292
K/K
none
293
black butt
H2S
294
Which test needs trytophan and is called Kovacs reagent
Indole
295
Citrate tests for microbes that
use citrate for their sole source of carbon
296
Kleb is citrate...
pos
IMViC
--++
297
E.coli is citrate
neg
IMViC
++--
298
Indicator that tests the ability of microbes to make glucose and drop the pH
methyl red
299
EMB selects and diff these group of microbes
enterobactericae
300
EMB has lactose to diff, what color is E.coli
color of NLF
green, LF
clear
301
Test that tests microbes ability to make acetone from glucose
Voges Proskauer
302
Voges Proskauer
2 pos: K/E
2 Neg: E/Y
Pos: Kleb, Enterobac
Neg: E.coli, Yer
303
In PPA, phenylalanine is deaminated to...
three PPA pos...PPM
phenylpyruvid acid
Proteus
Providencia
Morganella
304
Enzyme that breaks down aminos to amines (alkaline) w/limited glucose
first ph turns yellow
when decarboxylized alkaline amine turns purple
Decarboxylase
Decarboxylation occurs in the presence of a decarboxylase enzyme that catalyzes the breaking of the bond that binds the carboxylic group to the rest of the amino acid.
305
Aminos used in decarboxylase test
Lysine
Arginine
Orthinine
306
Positive nitrate reduction test changes what color and inidicates...
positive changes red, indicates has only nitrate reductase
307
Initial no color in negative nitrate reduction test indicates
No color may mean microbe has both or neither enzymes nitrate/nitrite reductase
308
How to confirm no color reaction in nitrate reduction test....
red..
no color..
add powered zinc, will catalyze nitrate to nitrite
red...has neither enzyme
clear...has both nitrite/nitrate
309
To ferment lactose need these two enzymes
permease:
brings into cell
B-galactosidase:
breaks down lactose
310
What are microbes called that lack permease but have B-galactosidase
slow lactose fermenters
311
Which test detects B-galactosidase
What does pos look like
ONPG
it resembles lactose
pos=yellow
312
Sodium deoxycholate is used in
String test for vibrio choloera
313
Test/medium that uses low peptone to allow visible acid production by oxidizers
OF
oxidition fermentation medium
314
With OF medium, what color is fermenters...
where is the color for oxidizers...
acid, yellow
oxidziers only on top due to needing oxygen
315
What is the indicator in OF media
bromthymol blue
316
Clostridium spores:
oval/sub:
round:
Clostridium spores:
oval/subterminal: bot,diff, sometimes perf
round terminal: tetani, ram
317
Obligate intracellular parasite w/anthropod vector
GNCB that resemble bacteria
Weil-Felix (proteus ag)
PCR lab titer
Rickettsia
318
Coxiella Burretii
obligate intracellular parasite
Q fever
aerosol from birth of infected
bioterrism
319
Two genus that have intracellular morulae in cells/WBC and transmitted by ticks
Ehrlichia chaffeensis:
mono/neu
Anaplasma phagocytophilum:
grans
320
Lyme disease, bulls eye rash
Ixodes dammini
serological
Borelia burgedorferi
321
obligate intra parasite
STD:
parrots:
PCR
Chlamydia
trachomatis
psittaci
322
D test
double diffusion
D appearance due to flattened zone of inhibition, resistant to clindamycin
323
Disk diffusion req
agar 4.0mm
MH 150mm
no more than 12 disks
no CO2
pH 7.2-7.4
Ca/Mg
324
Ingredient in McFarland
Barium Chloride
325
K/Vi antigen
Capsular antigen
Vi is K, made by S.typhi
Polysaccharide, heat labile, prevents phago
326
H antigen
Flagellar
Serotype Salmonella
Protein, heat labile
327
O antigen
sOmatic
Salmonella and Shigella
Lipopolysaccharide
Heat stable
328
Opportunistic enterobacteriacea in reptiles, fresh water fish, H2S, indoor, motile
++--
Edwardsiella tarda
329
3 enterobac with ++--
P.vulgaris
E.coli
Edwardsiella
330
3 enterobac with --++
Kleb pneu
Enterobacter
Serratia marcesens
331
Enterobacteriaciae that swarms
Proteus
332
SPEC is H2S
Salmonella
Proteus
Edwardsiella
Citro
333
3 Nonmotile entero at 35C
Shigella
Kleb
Yersenia
334
Pseudomonas aeruginosa, Acinetobacter,
Stenotrophomonas maltophilia
are all
Nonfermenting gram negative rods
335
Which one is NF
Pseudomonas aeruginosa, Acinetobacter,
Stenotrophomonas maltophilia
Acinetobacter of skin, pharynx
336
Two GNR, cause gastroenteritis, doesn't grow on TCBS, oxidase pos, motile
Aeromonas
Plesiomonas shigelloides
337
Ornithine pos selects for
Enterobacter
338
TSI:
SEEK A/A
Serratia
Eschericha
Enterobacter
Kleb
339
K/K
Pseudomonas
340
Fastidious, Capnophlic, GNCB
Oral flora
HACEK
341
HAECK, GNCB, pits agar, bleach odor
Eikinella corrodens
342
3 GNR Enterobacteriaceae with IMViC --++
Kleb pneumo
Enterobacter
Serratia
343
3 GNR Enterobacteriaceae with IMViC ++--
Proteus
E.coli
Edwardsella
344
Kleb versus Serratia
Motility, lactose
Kleb nonmotile, LF
Serratia motile, NLF
-most entero are motile
345
Of these 3 no fermenting GNR, which one is oxidase pos
Pseudo aero
Acinebacter
Stenotro
Pseudo
346
2 Enterobacteriaceae that produce H2S
Salmonella
Proteus
347
3 Enterobacteriaceae that has deaminase/PPA pos and urease pos
Proteus
Providencia
Morganella
348
Enterobacteriaceae that is DNase pos
Serratia
349
3 Enterobacteriaceae that are
- -++/VP pos
Kleb
Enterobacter
Serratia
350
Test that diff GPC from GNDC, reactions
Oxidase
GPC(staph, strep): oxidase neg
GNDC(neisseria): oxidase pos
351
Of the species of mycobacterium which two aren't rapid growers
gordonae, tb
352
Lactose fermenting enterobacteraciea, acrynom/organisms
CEEK (seek w/a C)
Citrobacter
Enterobacter
E.coli
Kleb
353
M.gordonae is slow
M.gordonae is slow
354
4 GPR anaerobic acrynom, names
Clostridium
Lactobacillus
Actinomyces
Propionibacterium
355
Only GN cocci anaerobic
Veillonella
356
4 GPR nonspore aerobic
1 GPR spore aerobic
Corneybacterium
Listeria mono
Erysipelothrix
Nocardia
spore...
Bacillus
357
Campy
Heli
Vibrio are all
GNR
358
Hemophilus is what shape/color
GNCB
359
Bartonella and Legionella gram stain
GNR
360
Brucella, Franciella, and Pasterurella gram stain
GNCB
361
Clostridium, Actinomyces,
Propionibacterium,
Lactobacillus all are ...., gram stain
GPR anaaerobic
362
Two organisms that are most common causes pelvic inflammatory diseases
N.gonn
Chlamydia trachomatis
363
Small, nonmotile
Coccobacillus
Grows in egg yolk sac
Rickettsia
364
2 organism have Bacterial type cell wall
Chlamydia: no peptidoglycan
Rickettsia
365
Naglar agar selects for
Clostridium
366
GNR, fastidious, Air conditioner
Best medium for legionella..
L.pneumo forms brown pigment on...
BCYE: buffered charcoal yeast extract has cysteine
L.pneumo: feeley gorman iron cysteine
367
Bordetella that is urease, oxidase pos
Bronchiseptica
368
4 ways to id mycobacteria and optimal atmosphere/% and temp
Growth rate
Pigment
Biochem
Temp
CO2, 5%
35C
369
Rhodococcus equi is GPC and takes...to go from coccoid to rod
24hrs
370
Blood cysteine glucose agar used for
Fran. tularensis
371
β-Lactamases are enzymes that degrade the β-lactam ring. They can be encoded chromosomally or on extrachromosomal elements.
This process of testing for enzyme by subculturing is called...
Plasmid coding
372
Name of test where DNA is hydrolyzed and what positive looks like
DNase
Medium is colorless around organism
373
In chromogenic cephalosporin test for betalactamase, the intact beta lactam ring is...
If organism cleaves ring it turns ..
Clear/yellow=intact
Red=cleaved
374
Sign of Lyme disease at site of tick bite called
ECM
Erythema chronicum migrans
375
Disease that causes raised, red, itchy rash (larva currens), often between your butt cheeks, on your thighs or on your wrists.
strongyloidiasis
376
larva currens is also called
strongyloidiasis
377
A red rash that usually begins on the face or neck, later spreading to the chest, trunk, arms and legs is called and caused by
Scarlet fever
GrpA step
378
Bulls eye is also called...
Seen in what disease...
Erythema chronicum migrans
Lyme
379
Mycosel is...with..and...
It is a highly selective media for the isolation of pathogenic fungi from materials having a large flora of other fungi and bacteria. It is not general purpose media for the isolation of all fungi (including molds and saprophytic yeasts).
Sabouraud Agar with Chloramphenicol and Cycloheximide
380
Trichophyton that is urease-pos and penetrates hair shaft
Mentagrophytes
381
Tuberculate macroconidia seen in what phase..
Of what organism...
Mycelial phase
H.capsulatum
382
Broad based budding yeast seen in... Phase of ..
Tissue phase
Blasto dermatitis
383
Alternating barrel shaped arthroconidia seen in this phase... Of...
Mycelial
C.immitis
384
Round, thick walled spherule with endospores seen is what phase and organism
Yeast
C.immitis
385
Mariners wheel buds seen in what phase and organism
Yeast
Para brasiliensis
386
Cutaneous larva migrans, creeping eruption caused by
Ancyclostoma braziliense
387
Creeping eruption called... By....
Cutaneous larva migrans
Ancyclostoma braziliense
388
Capillariasis
The nematode (roundworm) Capillaria (=Paracapillaria) philippinensis causes human intestinal capillariasis.
Unlike C. hepatica, humans are most likely the main definitive host. Transmission occurs primarily through eating undercooked fish.
389
eosinophilic meningitis, eosinophilic meningoencephalitis, or EM.
A. cantonensis:
People can get infected by eating raw or undercooked snails or slugs or contaminated produce.
B. procyonis: People get infected by accidentally ingesting infectious parasite eggs. These eggs can be found in raccoon feces and environments (such as dirt) contaminated with raccoon feces.
G. spinigerum: People can get infected by eating raw or undercooked freshwater fish or eels, frogs, poultry, or snakes
390
In hookworm and strongly they infect by ... ... With what form
Skin penetration
Filariform
391
best way to id mycoplasma
inhibition of growth by specific antisera
The growth inhibition test stems from the fact that high titre antiserum added into mycoplasma growth medium will inhibit the growth of the homologous mycoplasma species against which the antiserum was produced.
A method of mycoplasma species identification is described, based upon inhibition of the PPLO growth on solid media around antiserum-impregnated filter paper disks.
392
Name for bacteria that ferment lactose into acid, turn media pink
GN bacteria are defined as rod-shaped gram-negative nonendospore forming bacteria that ferment lactose with the production of acid and gas at 35–37°C.
Coliform
393
CLEN versus CLAP
Each letter...
CLEN: GPR aerobic
Corney, Listo, Eryt, Nocardia
CLAP: GPR anaerobic
Clostridium, Lacto, Acinto, Propio
394
phase where growth ceases due to nutrients exhausting and toxic metabolites build up
stationary
395
phase with litte/no multiplication but active enzymes, still maturing
lag
396
phase where theres a logarithmic, doubling of growth/binary fision
exponential
397
phase where waste builds up, nutrients depleted and cells stop metabolic functions and dye
death, declining
398
Most effective form of sterilization, high pressure, high temp
autoclave
15lbs, 15min, 121C
399
sterlization that uses moist heat-fractional alternate heating,
heat, sit, heat, sit
fractionalization
399
sterlization for proteineous things that can't be heated
filtration
399
sterlization at 100C, 15-30min, doesn't kill all spores viruses
boiling
400
Dry heat/flame and ethylene oxide gas are two forms of
sterlization
401
process that removes, kills, deactivates all life
sterilization
402
Dry versus moist QC species,
Both are genus...
Species...
Bacillus
Dry: subtilis
Moist: stearo-thermophilus
longer word, longer species
403
Process that inactivates bacteria on nonliving surfaces
disinfectant
404
Method using chemicals/antiseptics to destroy germs that cause infections on living tissues
antisepsis
405
In acid fast, what is red, what is blue
red=acid fast bacteria
blue=nonacid fast, background
406
Part of gram stain that makes dye less soluble to help dye adhere to cell wall
iodine
407
sodium polyanethol sulfonate
SPS used to...
grow bacteria
408
Pathogenic alpha strep in upper respiratory
Strep pneumo
408
SPS grows bacteria by preventing these three
prevents clotting, phagocytosis and bacterialcidal affect of human serum
409
Most abundant NF in throat
alpha heme strep (virridans)
410
Group A beta strep best collected with
throat swab
410
H.influen, N.mening, B.pertusus best collect with
nasopharyngeal swabs
upper respiratory
411
Most common pathogen in throat
beta strep
S.pyogenes
412
Positive coagulase test is
clotting of plasma
coagulase reacts with plasma/fibrinogen
412
Sensitive and faster way to test CSF for bacterial antigen
latex agg
413
2 GPC that are catalase positive
staph, micrococcus
414
2 GPC that are catalases neg
Strep, entero
415
Hippurate hydrolysis differentiates...
Hippurate hydrolysis postive in these two...
Diff: Group B from Group A
Pos: Group B and L.mono
416
GPC
Nonhemolytic
Catalase pos, coag neg
susceptible to novobiocin
Staph epi
417
GPC
beta hemolytic
Bacitracin sensitive
PYR +
necrotizing fascitis
Grp A
Strep pyogenes
418
GPC
beta hemolysis
Hippurate+/CAMP+
Neonatal meningitis/sepsis
GrpB
Strep agalactiae
419
GPC
CNS that is resistant to novobiocin...
CNS that is sensitive to novobiocin...
resist: sapro
sensitive: epi
420
GPC
CNS
catalase +
Novobiocin resistant
UTI young woman
Staph sapro
421
GPC and GNDC can be separated by this test
oxidase
422
Neissera/Moraxella: GNDC, oxidase...
Staph/Strep: GPC, oxidase...
Neissera/Moraxella: oxidase pos
Staph/Strep: oxidase neg
423
GPC, lancet,capsule
alpha heme
sensitive to optochin, bile soluble
Strep pneumo
424
3 Group D streps
Strep bovis, gallo
Enteroccocus
425
of the 3 strep, which one likes NaCL
All are....pos
enterococcus loves salt
bile esculin pos
426
Neissera morph...
Haemophilis morph...
Neisseria is GN cocci
Haemophilis is GN rod
427
GNDC with adjacent flat sides what genus
Neisseria
428
Both Thaymer martin and modified TM have these 4 things
Colistin, nystatin, vanco
in chocolate agar
429
What does Modified TM have that TM doesn't
trimethoprim lactate
inhibits swarming of proteus
430
Neisseria species all degrade...
But can differentiate speicies with these two sugars...
all degrade glucose
differentiate with maltose and lactorse
431
N.gonn degrades...
N.men degrades...
N.lac degrades...
gonn: glucose
men: glucose, maltose
lac: glucose, maltose, lactose
432
GNDC
oxidase positive
DNase,Butyrate +
middle ear
Morexalla catarrhalis
433
Corneybacterium diphtheriae morph, motility, salicin,
GPR
non motile
Salicin neg
434
GPR
nonmotile
BabesErnst
gray black on tellurite
grows on Loeffler
Corney diph
435
Tellurite in K-tellurite inhibits...
selects...
inhibits NF, gp/gn
selects C.diph
436
Medium that enhances pleomorphism, granules for Corney diph...
Medium where corney diph is gray-black...
Loeffler serum again
K-tellurite
437
E-test...
Elek test..
E-test: antimicrobial test
Elek: detect Corn diph toxin
438
Gram positive aerobic, rest aerobic are GN
-Cocci
-Rod
Cocci:
-staph, strep, micrococcus
-entero, group D(bovis,gallo)
Rod: BCLEN
-Bacillus
-Corny, Listeria, Erysi, Nocardia
439
Gram positive anaerobic, rest are GN
-Cocci:
-Rods:
cocci: peptococcus
Rods: CLAP
-Clostri,Lacto, Acinto,Propi
440
Most common GNR anaerobic
Bacter fragilis
441
Diphtheroids are corneybacterium that are NF in throat, which species is NF in throat
C.pseudo-diphtheriticum
442
GPCB
coccocus to rod in 24hrs
R.equi
equivalent with coccus and rod
443
GP to gram variable CB
cold/icecream, motile at 25
catase +,CAMP+,hipp+
Listo. mono
444
Two genus diff by motilty and salicin
Listeria and Corney
445
motile and salicin pos...
nonmotile and salicin neg...
pos: L.mono
neg: Corney
446
Strict anaerobes that are acid fast and need 5% CO2
myco-bacterium
not myco-plasma (no cell wall)
447
Middlebrook 7H11 used for...
color...
Lowenstein-Jensen used for..
color...
Middle: mycobacteria, clear
LJ: mycobacteria, green
448
Tween 80 diff...
pos..
neg...
diff Mycobacterium kansasii
pos: pink/red
neg: amber
449
Niacin/nitrate diff these two mycobacterium
M.tb from M.bovis
450
Mycobaterium tb naicin/nitrate...
M.bovis niacin/nitrate...
M.tb pos
Mbovis neg
451
Anaerobic organism that causes lumpy jaw
A.israelii
452
Urease +
PUNCH has urease in it
Proteus
Urea-plasma, duh!
Nocardia
Crypto neo/Corny
Heli
453
GPR
part of BCLEN(gram positive aerobic rod)
will not grow with antibiotics
partially acid fast
beaded, branching, filamentous
urease, catalase pos
Nocardia
454
GNR from nasopharengeal swab
-fastidious, small
-filamentious
H.influe
455
Haemophilus genus needs...
-influ needs...
-para needs...
-ducreyi needs...
enriched choc agar
influ: X,V
para: V
ducreyi:X
456
Haemophilus will satellite on BAP with Staph streak because
Staph supplies the V and then hemolysis will suppply the X
457
GNR
fastidious
animal bite
P.multo
458
Cause of:
-whopping cough...
-meningitis, flu, epiglottis..
-Pontiac...
-whopping cough: B.pertusis
-meningitis, flu, epiglottis: H,influ
-Pontiac: Legionella pneumo
459
Bordetella that is urease/oxidase pos
bronchiseptica
460
Brucella that doesn't make H2S
melitensis doesn't make H2S
461
Brucella that is inhibited by thionin
arbortus inhibited by thionin
462
GNR
fastidious
brown on feely-gorman iron cysteine
Legionella pneumo
needs cysteine
463
BCYE used for
Legionella
464
GNR fastidious
rabbits, bites, bioterrism
Franciella
tularensis
465
GNR fastidious in ACs
Legionella pneumo
466
Campylobacter is...
Needs...%....%....%
micro-aerophillic
85% N
10% CO2
5% O (least amt)
467
GNR curved
gull winged appearance, motile
microaerophillic, needs increased N at 42C
also nitrate pos and nonfermentative but who the hell is going to remember that
Campy jejuni
468
Campy jejuni uses what medium if enteritis
campy blood agar duh!
469
Ass with peptic ulcer
urea pos
Helicobacter pylori
470
Epi cell with gram variable bacteria
clue cell
471
Pleomorphilic, gram variable
NF vagina, clue cells, sniff test
Gardenella vaginalis
472
SS 4 media
XLD
SS
HE
GN broth
473
SS media ingredients
XLD:
SS:
HE:
GN broth:
XLD: xylose, lysine, deoxycholate
SS: brilliant green, lactose
HE: bromtyhmol blue, acid fuchsin
-lactose/sucrose
GN broth: deoxcholate, citrate
474
Color of S/S on HE
clear, clear w/dot
475
Two H2S indicators in triple sugar agar...
ferric ammonium citrate and sulfate
476
IMViC
Indole, methyl red, VP, citrate
477
IMViC:
E.coli...
Kleb...
Ecoli: ++--
Kleb:--++
478
IMViC
3 that are ++--
3 that are --++
++-- PEE: proteus, E.coli, Ed
--++ KES: Kleb, Enter, Ser
479
In PPA, phenalalaine is ....to phenylpyruvid acid
deaminated
480
acrynon: Organisms that are PPA pos, deaminate phenylalanine
PPM
P:proteus
P:providen
M:morganella
481
3 aminos used in decarboxylase test that breaks down aminos to amines
LAO
lysine, arginine, ornithine
482
positive DNase is...
two DNase pos genera...
clearing around colony
M.catt, Serr
483
Nitrate reduction:
-change color immediately=
-no changed color imm, and clear w/zinc...
-clear imm, color after zinc...
-change color imm=pos
-clear, clear w/zinc=pos for both
-clear imm, color w/zinc=neg
484
Lactose fermenters needs these two enzymes to ferment lactose
Beta-galactosidase
Permease
485
Test that detects slow LF that lack permease but have Betagalastosidase
ONPG
486
Oxidation fermentation media has ... ... to allow visibility of acid production by oxidizers
low peptone
487
tubes used in OF...
One...w/...
one...
2
one with oil
one open
OF need oxygen and will grow on top/yellow
488
Most isolated nonfermenter
oxidase pos
green, grape smell
swimmers ear
Pseudomonas aeruginosa
489
GN nonfermentative NF of skin
acinetobacter calcoa
490
Nagler agar selects for
Clostridium perfringens
491
Bacteroides fragilis is resistant to
penicillin
492
Acronymn for anaerobic GPR/bacilli
CLAP
C=clostridium
L=Lacto
A=Acinto
P=Propioni
493
The only GPC anaerobe...
The only GNC anaerobe..
GPC: Peptococcus
GNC: Veillonella
494
Anaerobic GPR/bacilli that makes double zone of hemolysis
C.perfringens
495
Two Clostridium with round terminal spores
ramosum
tetani (tennis racket)
496
Two clostridium with oval subterminal spores; the other two that are important
botulinum
difficile
497
GNC
anaerobic
red flourescence
Veillonella
498
GPC
anaerobic
Peptococcus
499
Green zone, partial lysis
Strep pneumo, virridians
Alpha heme
500
Clear zone, complete lysis
GrpA ,GrB
Beta
501
Staph aureus resistant to...
Staph sapro resistant to...
Strep B resistant to....
GrpD virridians resistant to...
Staph aureus resistant to: penicillin
Staph sapro resistant to: novobiocin
-diff CNS from Sapro
Strep B resistant to: bacitracin
-diff A from B
Virridians resistant to: optochin
-diff pneumo from virridians
502
Acceptable anaerobic culture
B's: Blood, BM, BF, Bile
-not bronch washings
Aspirates
Tissue, wound
503
Oral flora, can cause endocarditis
Fastidious GNCB
Capnophillic
Acronym...
Haemophilis
Actino/Aggre
Cardio
Eikinella
Kingella
504
Most common anaerobic
GPR...
GNR..
GPR: Propioni-bacterium
-acne
GNR: Bacteroides fragilis
-BBE brown
505
Most common sexually transmitted BACTERIAL infection
Chlamydia trachomatis
506
Smallest free living cells with no cell wall
Myco-plasma and Urea-plasma
507
2 obligate intracellular parasits that have a bacterial type cell wall
chlamydia and rickettsia
508
GNCB that can grow in egg yolk sacs
Rickettsisa likes to sleep in yolk
509
Rickettsia transmitted by
arthropod vector, tick/louse
510
2 organisms that cause pelvic inflammatory disease
N.gonn
Chlamydia trach
gonnorhea and chlamydia
511
Two tick borne organisms with morulae in WBC
Ehrlichia and Anaplasma
512
bulls eye rash called...
seen in lyme disease at site of tick bite
ECM
erhythema chronicum
migrans
513
KB what is used to adjust turbidity of inoculum...
what is concentration...
made of...
McFarland std
0.5
barium chloride
514
After inoculating MH plates, dry for at least...to no more than...mins
3?5?, 30mins
515
In D test, double diffusion, there is a flattended "D" due to resistance to...
clindamycin
516
Lowenstein-Jenson good for...
Regan Lowe good for...
Loefflers Medium...
Lowenstein-Jenson good for... mycobacteria
Regan Lowe good for...
Bordetella pertussis
Loefflers Medium...
Cory diph
517
The growth inhibition test,
inhibition of growth by specific antisera
Used for...
Mycoplasma
