amoeba Flashcards
extraintestinal amoeba
E. gingivalis
N. fowleri
Acanthamoeba spp.
assoc with PAM
N. fowleri
assoc with GAE and keratitis
Acanthamoeba spp
with active varying MULTIPLE pseudopods (long short blunt)
E. gingivalis
has sluglike motility
BLUNT pseudopods
N. fowleri
has sluggish motility
SPINELIKE PSEUDOPODS (ACANTHOPODIA) - project outward from the base
Acanthamoeba spp
no. of nuclei of trophozoites and cysts of extraintestinal amoeba
consistently 1!!!
only amoeba that ingests WBC
E. gingivalis
only amoeba with NO CYSTS form
E. gingivalis
only amoeba with 3 morph form (trophozoites, cysts, flagellate)
N. fowleri
extraintestinal amoeba that resembles E. histolytica
E. gingivalis
broad anterior end
tapered posterior end
N. fowleri
pear shaped form
flagellate form of
N. fowleri
has jerky spinning motility
N. fowleri flagellate form
has DOUBLE CELL WALL CYST that aids in its ID
Acanthamoeba spp
(smooth inner, jagged outer)
spx used to both ID E. gingivalis and E. histolytica
pulmonary abscess
sputum
method of choice for N. fowleri trophozoites
CSF micro exam
its trophozoites has TRAILING EFFECT on agar pkates inoculated with gram neg bacilli
N. fowleri
technique of choice to speciate Acanthamoeba
Indirect Immunofluorescent Ab stain
specimen of choice for Acanthamoeba troph and cyst
CSF
specimen of choice for Acanthamoeba eye inf
corneal scrapings
produce TRACKS as they feed on a non-nutrient agar plate seeded with E. coli
Acanthamoeba spp.
stains Acanthamoeba cysts in corneal scrapings
Calcofluor white (for histo exam)
first amoeba recovered from human spx
E. gingivalis
only form known to exist in humans
Trophozoites of N. fowleri
known to exist only in external environment
Cyst of N. fowleri
Naegleria spp that could infect humans in the future
Pathogenic in mice
Intranasal installation
N. australiensis
aids in taxonomix classification of N. fowleri and Acanthamoeba
DNA RFLP tests
responsible for most CNS and Eye infection
Acanthamoeba castellanii
assoc with contact lens wearer
Acanthamoeba
shares many characteristics with gram neg PSEUDOMONAS AERUGINOSA
Acanthamoeba
intestinal amoeba
E. histolytica
E. hartmanni
E. coli
E. polecki
E. nana
I. butschlii
intestinal amoeba assoc with:
Intestinal amebiasis
Amebic colitis
Amebic dysentery
Extraintestinal amebiasis
E. histolytica
E. hartmanni
E. coli
rapid, UNIDIRECTIONAL, progressive
finger-like hyaline pseudopods
E. histolytica
Nonprogressive
Finger-like pseudopods
E. hartmanni
Sluggish, nonprogressive
BLUNT pseudopods
E. coli
intestinal amoeba trophozoites nucleus no.
1!!
their CYSTS consists of 1-4 nucleus
4 is usually MATURE INFECTIVE CYSTS AND SMALLER
E. histolytica
E. hartmanni
E. nana
intestinal amoeba with consistently having 1 nucleus in both troph and cysts stage
E. polecki
I. butschlii
cysts may have 1-8 nucleus or greater than 16
E. coli
has ground glass cytoplasm
E. histolytica
ingested RBCs
E. histolytica (diagnostic feature)
beaded appearance of peripheral chromatin
E. hartmanni
has HYALINE CYSTS WALL
E. histolytica
has THIN chromatoid bars with pointed to splintered ends
E. coli
young cysts have diffuse glycogen mass that may DISPLACE NUCLEI (often seen in cysts with 2 nuclei)
E. coli
special medium for E. histolytica
TYI-S-33
size ranges overlap; requires OCULAR MICROMETER
E. histolytica
E. hartmanni
prevalence is similar to that of E. histolytica
E. hartmanni
only known PATHOGENIC INTESTINAL amoeba
E. histolytica
vector borne intestinal amoeba
E. histolytica
hartmanni
coli
NONPATHOGENIC
morphologically identical to E. histolytica
E. dispar
small race E. histolytica
E. hartmanni
has SLUGGISH PROGRESSIVE motility in normal stools
has PROGRESSIVE, UNIDIRECTIONAL motility in diarrheal stools
E. polecki
nuclear structure resembles E. histolytica and E. coli
E. polecki
Key feature is ABSENT PERIPHERAL CHROMATIN
E. nana
also I. butschlii
has SLUGGISH NON PROGRESSIVE motility
BLUNT HYALINE PSEUDOPODS
E. nana
has SLUGGISH PROGRESSIVE motility
I. butschlii
large irregular blotlike karyosome
E. nana
has ABSENT chromatoid bar
E. nana
I. butschlii
feature seen in Entamoeba
has CHROMATOID BARS
has WELL DEFINED GLYCOGEN MASS with DEFINITE BORDERS
I. butschlii
glycogen mass typically picks up IODINE STAIN
I. butschlii
parasite of pigs and monkeys
E. polecki
only discomfort for symptomatic px is diarrhea
E. polecki
acquired from contaminated HOG feces
I. butschlii
nucleus does NOT undergo typical division (diff from other intestinal amoeba)
Iodameoba
cyst nucleus: BASKET OF FLOWERS
I. butschlii