amoeba Flashcards
extraintestinal amoeba
E. gingivalis
N. fowleri
Acanthamoeba spp.
assoc with PAM
N. fowleri
assoc with GAE and keratitis
Acanthamoeba spp
with active varying MULTIPLE pseudopods (long short blunt)
E. gingivalis
has sluglike motility
BLUNT pseudopods
N. fowleri
has sluggish motility
SPINELIKE PSEUDOPODS (ACANTHOPODIA) - project outward from the base
Acanthamoeba spp
no. of nuclei of trophozoites and cysts of extraintestinal amoeba
consistently 1!!!
only amoeba that ingests WBC
E. gingivalis
only amoeba with NO CYSTS form
E. gingivalis
only amoeba with 3 morph form (trophozoites, cysts, flagellate)
N. fowleri
extraintestinal amoeba that resembles E. histolytica
E. gingivalis
broad anterior end
tapered posterior end
N. fowleri
pear shaped form
flagellate form of
N. fowleri
has jerky spinning motility
N. fowleri flagellate form
has DOUBLE CELL WALL CYST that aids in its ID
Acanthamoeba spp
(smooth inner, jagged outer)
spx used to both ID E. gingivalis and E. histolytica
pulmonary abscess
sputum
method of choice for N. fowleri trophozoites
CSF micro exam
its trophozoites has TRAILING EFFECT on agar pkates inoculated with gram neg bacilli
N. fowleri
technique of choice to speciate Acanthamoeba
Indirect Immunofluorescent Ab stain
specimen of choice for Acanthamoeba troph and cyst
CSF
specimen of choice for Acanthamoeba eye inf
corneal scrapings
produce TRACKS as they feed on a non-nutrient agar plate seeded with E. coli
Acanthamoeba spp.
stains Acanthamoeba cysts in corneal scrapings
Calcofluor white (for histo exam)
first amoeba recovered from human spx
E. gingivalis
only form known to exist in humans
Trophozoites of N. fowleri
known to exist only in external environment
Cyst of N. fowleri
Naegleria spp that could infect humans in the future
Pathogenic in mice
Intranasal installation
N. australiensis