Amniotic Fluid Flashcards

1
Q

Amniotic fluid is Found around the developing fetus inside a membranous sac called

A

Amnion

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2
Q

Main function of amniotic fluid

A
  • cushion and protect the developing fetus
  • serves a key role in the exchange of water and molecules between the fetus and the maternal circulation
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3
Q

Amniotic fluid is formed from the

A

Placenta

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4
Q

Volume of amniotic fluid increases steadily throughout the pregnancy up to a maximum of

A

1100-1500 mL at 36 weeks of gestation.

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5
Q

When fetal urine production begins, the chemical composition of the amniotic fluid changes

A

Increased production creatinine

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6
Q

At commencement of fetal urine production, fetal swallowing of amniotic fluid begins and this regulates the formation of

A

fetal urine.

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7
Q

Decreased fetal swallowing resulting in increase amniotic fluid volume

A

HYDRAMNIOS:

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8
Q

Decreased amounts of amniotic fluid. Can occur with premature rupture of the membranes and Congenital malformations

A

OLIGOHYDRAMNIOS:

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9
Q

amniotic fluid volume greater than 1200 mL is

A

Polyhydramnios

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10
Q

excessive accumulation of amniotic fluid, amniotic fluid volume greater than 1200 mL is

A

Polyhydramnios

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11
Q

collection of amniotic fluid

A

AMNIOCENTESIS

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12
Q

Amniocentesis can be collected either by

A

Transabdominal or transvaginal

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13
Q

Needle aspiration is introduced into the amniotic sac, usually transabdominally with simultaneous use of

A

Ultrasound

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14
Q

The addition of ultrasound has helped to make the procedure safer especially if performed between

A

15 - 18 weeks of gestation for genetic studies

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15
Q

Amount of amniotic fluid collected is usually

A

10-20 mL (max of 30)

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16
Q

less desirable as cells have more tendency to adhere to its surface

A

Glass containers

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17
Q

Color of normal amniotic fluid

A

Colorless to pale yellow

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18
Q

Amniotic fluid is slightly turbid due to

A

Fetal cells, vernix, hair

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19
Q

(protective coating that forms on baby’s skin in utero)

A

Vernix

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20
Q

Specimen for Cell Culture and Chromosomal Studies

A
  • stored at Room temperature
  • to keep the fetal cells alive
21
Q

Specimen for phospholipid analysis

A

transported on ice and centrifuged at 500 g and the supernatant saved for testing.

22
Q

if blood is present in specimen for phospholipid analysis

A

specimen should be centrifuged to prevent hemolysis from altering the test results

23
Q

Specimen for chemical analysis

A

Stored at any length of time but must be centrifuged.

24
Q

If samples for chemical analysis need to be stored more
24 hours, they must be

A

Frozen

25
Q

Microscopic test to differentiate amniotic fluid from maternal urine.

A

Fern test

26
Q

In fern test - is spread out on a glass slide and allowed to dry at room temperature.

A

Vaginal fluid

27
Q

It is a test used to evaluate pre-mature rupture of the membranes.

A

Fern test

28
Q

Colorless amniotic fluid may indicate

A

Normal (MAY SHOW SLIGHT TO MODERATE TURBIDITY)

29
Q

BLOOD STREAKED

A

TRAUMATIC TAP, ABDOMINAL TRAUMA, INTRA AMNIOTIC HEMORRHAGE

30
Q

YELLOW

A

HEMOLYTIC DISEASE OF THE NEWBORN

31
Q

DARK GREEN

A

MECONIUM

32
Q

DARK RED BROWN

A

FETAL DEATH

33
Q

L/S RATIO (Lecithin/Sphingomyelin) principle

A

Thin Layer Chromatography

34
Q

Amniostat FLM principle

A

Agglutination immunoassay

35
Q

L/S RATIO (Lecithin/Sphingomyelin) details

A

Sphingomyelin is used as internal standard,

36
Q

Amniostat FLM details

A

Uses antisera specific to phosphatidylglycerol.

37
Q

greatly affected by blood and meconium contamination

A

L/S RATIO (Lecithin/Sphingomyelin)

38
Q

Not affected by blood and meconium contamination

A

Amniostat FLM

39
Q

L/S RATIO (Lecithin/Sphingomyelin) NORMAL VALUES:

A

> 2.0

40
Q

NORMAL VALUES: of amniostat FLM

A

Positive

41
Q

FOAM STABILITY INDEX principle

A

Modified Foam shake

42
Q

FOAM STABILITY INDEX details

A

95% ethanol used as anti-foaming agent

43
Q

Normal values of foam stability index

A

> 47

44
Q

MICROVISCOSITY principle

A

Fluorescence polarization

45
Q

MICROVISCOSITY normal values

A

> 55 mg/g

46
Q

MICROVISCOSITY details

A

Albumin used as internal standard

47
Q

LAMELLAR BODY COUNT principle

A

Resistance pulse counting

48
Q

LAMELLAR BODY COUNT details

A

Uses the platelet channel of hematology analyzers

49
Q

LAMELLAR BODY COUNT normal values

A

> 32,000/mL