AML Introduction Flashcards
1
Q
What cytogenetic abnormalities are sufficient to
diagnose AML with MDS-related changes ?
A
- complex karyotype (3 or more chromosomal abnormalities)
- Unbalanced changes
- Balanced changes
Note: presence of NPM1 or biallelic mutations of CEBPA excludes AML from this category.
see pg. 378 flow book for unbalanced and balanced changes
2
Q
What are the precursor blastic
markers or precursor stage markers ?
A
- CD34
- CD38
- CD117
- CD133
- HLA-DR
- TdT
3
Q
What are considered to be myeloid
markers ?
A
- cytoplasmic MPO
- CD13
- CD33
4
Q
When diagnosing an MPAL
(mixed phenotype acute leukemia), what
myeloid lineage markers are needed?
A
- MPO
- OR evidence of monocytic differentiation, with expression of at least 2 monocytic markers:
- NSE
- CD11c
- CD14
- CD64
- Lysozyme
5
Q
When diagnosing an MPAL
(mixed phenotype acute leukemia), what
B-lineage markers are needed?
A
- CD19 (strong)
- with at least one of the following:
- CD79a
- CD22
- CD10
- CD19 (weak) with at least two of the following:
- CD79a
- CD22
- CD10
6
Q
When diagnosing an MPAL
(mixed phenotype acute leukemia), what
T- lineage markers are needed?
A
- CD3 (surface or cytoplasmic)