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CCP4.1: Pharmaceutical Biochemistry, Lab > Amino Acids > Flashcards

Amino Acids Flashcards

1
Q

building blocks of proteins

A

Amino acids

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2
Q

organic molecule containing both carboxyl acid and amino functional groups

A

Amino acids

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3
Q

can be grouped as nonpolar,polar,acidic,basic

A

Amino acids

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4
Q

capable of being converted to glucose/sugar

A

Glucogenic Amino acid

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5
Q

capable of being converted to ketone

A

Ketogenic Amino acid

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6
Q

a process of changing/deviating from the native conformation

A

Denaturation

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7
Q

Denaturing agents:

A
  1. Heat
  2. Extreme pH
  3. Mercaptoethanol
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8
Q

constitute of 70% of the organic matter of a cell

A

Proteins

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9
Q

are polymers of amino acids linked by peptide bonds

A

Proteins

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10
Q

globular:

A

globule (ex.albumin)

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11
Q

fibrous:

A

large

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12
Q

structural protein;made up of 2AA

A

Collagen

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13
Q

blood vessels

A

Elastin

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14
Q

storage proteins, muscles

A

Myoglobin

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15
Q

transport proteins, carries O2 blood

A

Hemoglobin

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16
Q

hormones;controlling blood sugar

A

Insulin & Glucagon

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17
Q

antibodies

A

Immunoglobulins

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18
Q

Isolation/Sequencing of Proteins

A
  1. By polarity: salting out, precipitation
  2. By charge
  3. By size
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19
Q

technique of separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream

A

Chromatography

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20
Q

_______phase may be either a liquid or a gas

A

Mobile phase

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21
Q

________phase is either a solid or a liquid

A

Contiguous stationary phase

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22
Q

Mobile phase: Non-polar
Stationary phase: Polar

A

Normal

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23
Q

Mobile phase: Polar
Stationary phase: Non-polar

A

Reverse

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24
Q

Xanthoproteic Test: Reagent

A

HNO3

25
Q

Xanthoproteic Test: Positive

A

Yellow or Orange ppt

26
Q

Xanthoproteic Test:Presence

A

Phenylalanine

27
Q

Fohl’s Test: Reagent

A

PbCH3COO

28
Q

Fohl’s Test:Positive

A

Black ppt (PbS)

29
Q

Fohl’s Test:Presence

A

Methionine

30
Q

Hopkin-Cole Test:Reagent

A

Glyoxylic acid

31
Q

Hopkin-Cole Test:Positive

A

Violet ring

32
Q

Hopkin-Cole Test:Presence

A

Tryptophan

33
Q

Ninhydrin Test:Reagent

A

Ninhydrin rgt

34
Q

Ninhydrin Test:Positive

A

Primary: Violet
Secondary: Yellow

35
Q

Ninhydrin Test:Presence

A

General test for free amino acids

36
Q

Millon-Nase:Reagent

A

Hg

37
Q

Millon-Nase:Positive

A

Pink/old Rose

38
Q

Millon-Nasse:Presence

A

Phenolic rings, Tyrosine

39
Q

Sakaguchi Test:Reagent

A

a-naphthol

40
Q

Sakaguchi Test:Positive

A

Red/wine color

41
Q

Sakaguchi Test:Presence

A

Arginine

42
Q

protein made by the liver

A

Albumin

43
Q

it keeps fluid from leaking out of blood vessels, nourishes tissues and transports hormones, vitamin, drug and other substances throughout the body

A

Albumin

44
Q

Disease related to albumin deficiency

A
  1. Severe liver disease
  2. Kidney disease
45
Q

family of proteins found in grains like wheat, rye, spelt and barley

A

Gluten

46
Q

two main proteins found in gluten

A

Glutenin and Gliadin

47
Q

most severe form of gluten intolerance

A

Celiac disease

48
Q

autoimmune disease that involves the body from treating gluten as an invader

A

Celiac disease

49
Q

Common sources of gluten:

A
  1. Wheat
  2. Spelt
  3. Rye
  4. Barley
  5. Bread
  6. Pasta
  7. Cereals
  8. Beer
  9. Pastries
50
Q

When wheat flour is washed this process removes the starch that when tested with I2 solution _____results give ______ solution

A

Positive results give violet or purple solution

51
Q

The ________ material left is the gluten

A

Insoluble material

52
Q

vital to oxygen transport and storage in vertebrates

A

Myoglobin and Hemoglobin

53
Q

dominant in muscles

A

Myoglobin

54
Q

dominant in blood

A

Hemoglobin

55
Q

myoglobin can be isolated by using _________precipitation

A

ammonium sulfate precipitation

56
Q

a common technique used to identify compounds, determine purities

A

Thin layer chromatography

57
Q

also used to separate or differentiate substances according to their polarity

A

Thin layer chromatography

58
Q

semi-quantitative method

A

Thin layer chromatography

59
Q

special finely ground matrix (silica gel, alumina) is coated on a glass plate or a metal or plastic film as a thin layer

A

Stationary phase: Thin layer chromatography

CCP4.1: Pharmaceutical Biochemistry, Lab (1 decks)

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