Altklausur Fragen Flashcards

1
Q

What is Evolution? What preconditions are essential for evolution to happen?

A

Descent with modification: All species have evolved from one single common ancestor and have then accumulated modifications through time which make all lineages different in the present.

“Evolution are heritable changes in populations over time”

Preconditions:
- Inherited similarity -> Evolution is a historical process
- Variation
- Selection

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2
Q

Which statement(s) on phylogeny is/are correct
1) The natural system is based on phylogenetic relationships
2) Any phylogenetic system must rely on symplesiomorphies
3) Apomorphies are used to identify monophyla
4) You cannot infer phylogenetic relationships from an unrooted
tree

A

Statement 1) and 3) are correct.

1) The natural system is based on phylogenetic relationships: This statement is correct. The natural system of classification seeks to reflect the evolutionary relationships among organisms, making it a phylogenetic classification.

2) Any phylogenetic system must rely on symplesiomorphies: This statement is incorrect. Phylogenetic systems rely on synapomorphies, which are shared derived characteristics among a group of organisms, rather than symplesiomorphies (shared ancestral characteristics).

3) Apomorphies are used to identify monophyla: This statement is correct. Apomorphies are derived characteristics that are unique to a particular clade or group of organisms, and they are often used to identify and define monophyletic groups.

4) You cannot infer phylogenetic relationships from an unrooted tree: This statement is incorrect. Phylogenetic relationships can be inferred from both rooted and unrooted trees. Rooting a tree helps to establish the direction of evolution, but unrooted trees can still provide information about the relationships among different taxa.

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3
Q

Mention the different geographic scenarios of speciation? What is the difference among them?

A

Allopatric Speciation:
occurs when populations of a species become geographically isolated from each other, often due to physical barriers such as mountains, rivers, or oceans.
Difference: the geographical separation, which leads to reproductive isolation and divergence between the separated populations.

Sympatric Speciation:
occurs when speciation happens within the same geographical area without any physical barriers. Reproductive isolation is often driven by factors other than geographical separation, such as ecological or behavioral differences.
Difference: populations are in close proximity, and the speciation process is not primarily driven by geographic isolation. Instead, it may be influenced by factors like niche differentiation or mate preferences.

Parapatric Speciation:
occurs when populations are distributed along a geographical gradient, and speciation occurs in areas of overlap. There is limited gene flow between adjacent populations.
Difference: there is a zone of overlap between populations, and speciation occurs along the edges of their ranges.

Peripatric Speciation:
is a special case of allopatric speciation where a small, isolated population on the periphery of a larger population undergoes speciation.
Difference: the isolated population is smaller and potentially faces different selective pressures, leading to divergence from the main population.

(Adaptive Radiation:
is a process in which a single ancestor species rapidly diversifies into a wide variety of forms to adapt to different environments or ecological niches.
Difference: While not strictly a geographic scenario, adaptive radiation often involves colonization of different geographical areas or ecological niches, leading to the evolution of various species.)

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4
Q

Which statement(s) on mutations/substitutions is/are correct?
1) Sexual reproduction leads to increases mutation rates
2) Natural selection acts on mutation rates
3) In exons, silent mutations are more common than expressed ones
4) In exons, silent substitutions are more common than expressed ones

A

1) Sexual reproduction leads to increases mutation rates: Incorrect! But: Sexual recombination helps a beneficial mutation shifting through a population (standing genetic
variation)
2) Natural selection acts on mutation rates: Incorrect! Natural selection primarily acts on the phenotypic effects of mutations rather than directly on mutation rates. But: There are “ideal” mutation rates -> too low: no evolution; too high: too many extinctions.
3) In exons, silent mutations are more common than expressed ones. Correct? In Exons, silent mutations are more common than non-silent ones.
4) In exons, silent substitutions are more common than expressed ones. Correct? In exons, silent substitutions are more common than expressed ones.

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5
Q

Which statement(s) on mutations/substitutions is/are correct?
1) The number of own offspring matters for individual fitness
2) High fitness means a large contribution to the gene pool of the
next generation
3) Reproductive fitness describes the body fitness of individuals, while reproducing
4) Without own offspring, the reproductive fitness is always zero

A

Statement 1), 2) are correct.

3) Incorrect! Reproductive fitness generally refers to an individual’s success in reproducing and contributing to the gene pool. It is not used to describe the physical fitness of an individual’s body.
4) Incorrect! Reproducive fitness alsoconsists of inclusive fitness.

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6
Q

In what groups of organisms may pesticide resistance evolve rapidly, and why?

A

Resistances evolve rapidly in short-lived organisms: insects, bacteria, annual plants!
-> Short generation times can undergo more reproductive cycles in a given time, allowing for the accumulation and spread of resistance alleles.
-> Large population sizes, high standing genetic variation
-> Mobility/ Dispersal of seeds and Diaspora

Evolution of resistance is an example of directional selection: occurs when individuals at one end of the phenotype range have higher fitness than individuals in the middle or the other end of the range.

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7
Q

A native and an invasive plant species cross with each other. Which two conditions are necessary for introgression and the formation of a hybrid swarm to occur?

A

1) Hybridization
2) Backcrossing and Gene Flow

Introgression only works in hybrids are fertile!

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8
Q

Explain mimicry with an example

A
  • phenomenon where one organism closely resembles another organism or object, usually for the purpose of gaining some advantage, such as protection from predators, improved access to resources, or increased chances of successful reproduction. Mimicry can take various forms, and it is often categorized into different types, including Batesian mimicry, Müllerian mimicry, and aggressive mimicry.

Example: Cuckook - agressive mimicry
1. Female does not boil own nest. It removes one host egg (host counts eggs) and lays egg in host nest (very fast egg deposition)
Egg looks like host egg & chick looks like host chick = Mimicry -> reducing the likelihood of the host birds recognizing and rejecting the cuckoo eggs.
2. Cuckoo chick hatches earlier than host chicks, removes other eggs/ chicks
3. Aggressive begging -> inner colour of beak is more vibrant (motivates “parent” to feed), shouts more often

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9
Q

Why is the concept of group selection – according to current knowledge – not accepted as explanation for cooperative behavior among conspecifics?

A

kin selection and individual selection are better explanations.

  • Benefit of other individuals only stable if individuals are long-lived(reciptocal altruism), or related (kin-recognition) or the species/ population has low dispersal potential (naked mole rats)
  • difficult to maintain altruism in population, because egoistic individuals would have the a fitness advantage -> evolution in egoistic direction
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10
Q

List one benefit and one disadvantages of EACH of these sequencing technologies: Illumina, Oxford Nanopore.

A

Illumina:
+ Illumina sequencing provides high accuracy in base calling. Low error rates
- the relatively short read lengths

Oxford Nanopore
+ produces long reads. -> advantageous for resolving complex genomic regions, repetitive sequences, and structural variations
- higher error rates compared to Illumina

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11
Q

What are the parts of the synthesis theory?
What are the requirements for evolution?

A

Evolutionary theory (Charles Darwin & Ernst Haeckel) meets genetics (Mendel, De Vries & Morgan).

„Mutations create the variation, which selection acts upon“ Dobzhansky and Huxley

Evolutionary theory: - Phenotypic variation
- Limited resources -> survival of the fittest (natural selection)
+
Mendelian: -new species through single mutations
- natural selection is old-fashioned speculation
- mutation and selection are mutually exclusive
- only discrete variation relevant

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12
Q

Explain the Red Queen hypothesis with an example

A

The idea is that in a changing environment, organisms must continuously evolve and improve to maintain their relative fitness and survive.

species must constantly adapt, evolve, and proliferate in order to survive in the face of ever-evolving and adapting competitors or predators.

Example: Host-Parasite Coevolution

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13
Q

What is mimicry? Give an example

A

phenomenon where one organism closely resembles another organism or object, usually for the purpose of gaining some advantage, such as protection from predators, improved access to resources, or increased chances of successful reproduction.

e.g. Cuckook, hoverfly

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14
Q

How does the Illumina NGS work?

A
  1. Sample prep
  2. Bind DNA to flowcell, generate clusters (bridge amplification)
  3. Sequencing by synthesis
  4. Data analysis
  • Ligation of adapters to each end of the DNA molecule
  • Single strands are coupled to glass slides, via adaptors
  • PCR done on slide to form “PCR colonies” or “polonies”
  • For subsequent sequencing, nucleotides are blocked, so no more than one can be incorporated per cycle
  • Four fluorescent dyes, detection of pictures
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15
Q

Name 2 factors that influence the maintenance of aDNA.

A

1) Degradation over Time:
Over time, DNA undergoes degradation due to various environmental factors such as exposure to moisture, heat, and UV radiation

2) Contamination from modern DNA sources (e.g., researchers, environmental sources) can compromise the integrity of aDNA.

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16
Q

Explain allopatric speciation with an example

A

Allopatric speciation is a process of speciation that occurs when a population of a species becomes geographically isolated from another population of the same species, leading to reproductive isolation and the evolution of distinct species over time.

Example: Allopatric Speciation in Island Birds

1) Initial Population:
Imagine a population of small songbirds living on a mainland.
2) Geographical Barrier Formation:
Over time, a geographical barrier, such as a wide river or mountain range, forms, dividing the bird population into two isolated groups.
3) Isolation:
The birds on one side of the barrier are separated from those on the other side. As a result, gene flow between the two groups is restricted.
4) Divergent Evolution:
In each isolated group, the bird populations face slightly different environmental conditions, food sources, and challenges. Natural selection and genetic drift start to act independently on the two groups.
5) Accumulation of Genetic Differences:
Over many generations, random mutations, genetic drift, and natural selection lead to the accumulation of genetic differences between the two populations.
6) Reproductive Isolation:
As genetic differences accumulate, the two populations may become reproductively isolated. This could be due to changes in mating behaviors, mating seasons, or physical traits.
7) “Formation of Two Species:”
Eventually, the two populations evolve to the point where they can no longer interbreed successfully, even if the geographical barrier is removed. They have become distinct species through the process of allopatric speciation.

(Example: Amphipods in New Zealand- analyzed at allozyme loci
Allozyme = alternative form of an enzyme that is coded by different alleles at the same locus)

17
Q

What fitness can hybrids have?

A

Hybrid phenotypes may
* have a reduced fitness
* have enhanced fitness (hybrid vigour = heterosis)
* enlarge the ecological niche of parental species, if
these differ in their niches

Heterosis: Heterozygotous genotypes perform better than parental homozygotous genotypes

18
Q

MC about fitness:
1) affects natural selection more on small populations or on large population?
2) individual fitness gets higher through jointly breeding with unrelated parents
3) Natural selection depends on the genes in the gene pool of the previous generation
4) Natural selection depends not only on environmental conditions but also on the genotype
5) the closer the offspring is related to you, the more of your fitness is in the next generation

A

1) Yes, more on small because of genetiv drift and less variability in small pop.
2) incorrect helping in offspring breeding only increases fitness if related
3) incorrect?
4) Natural selection acts on the variation in genotypes that affect an individual’s fitness in a given environment. The interaction between genotype and environment determines an organism’s ability to survive and reproduce.
5) Kin selection theory suggests that individuals can enhance their own fitness by helping close relatives who share a significant proportion of their genes. The closer the genetic relationship, the more shared genes, and therefore, aiding close relatives can be a strategy to increase one’s inclusive fitness.

Nochmal besprechen

19
Q

Herbicide resistant weed is found on field. Describe 2 possibilities what farmer could do to eliminate it

A
  • Change of active substances, or using a combination of different substances (rotation, but risk of cross-resistance)
  • Alternative methods for the control of pest organisms (e.g. biological control, „biocontrol“)
  • Promote natural enemies of pest organisms („Nützlinge“, birds, parasitic wasps, predatory insects)
20
Q

Invasive species affect ecosystems in an adverse way. Mention 4 mechanisms how that could happen.

A
  • Species declines
  • Degradation
  • Changes of ecosystem functions (nutrient cycles,
    microclimate)
  • Changes of soil properties
21
Q

3 differences between Illumina and high throughput sequencing techniques

A

1) Reading length
Illumina shorter reading length,
Oxford Nanopore longer reads

2) Quality
Illumina: Quality much higher,
PacBio and Oxford Nanopore have higher error rates

3) Principles
Illumina: sequencing-by-synthesis, involving detection of fluorescently labeled nucleotides as they are incorporated into the growing DNA strand.

Oxford Nanopore: Passes DNA through a protein (“nanopore”) -> during process electric
current is measured

4) Application
Illumina: applications requiring high accuracy and moderate read lengths

Oxford Nanopore: ability to produce long reads, making them suitable for applications such as de novo genome assembly and structural variant detection.

22
Q

Give an example for sexual selection and mention which selection type you described.

A

Intersexual selection- Epigamic selection
Traits increasig attractiveness:
- ornaments
- colours
e.g. Peacock tail feathers

Behaviour:
- display behaviour (e.g. song, dance, lekking)
- tenure

Intrasexual selection
Traits increasing competitive strength:
-Body mass
-Weapons
e.g. Antlers of a stag

Behaviour:
-fighting,
-rutting,
-Lekking (Arenabalz),
-mate guarding

23
Q

You want to sequence a skull. Which parts of it are suited best for sequencing?
Mention 2 factors that possibly can damage DNA and which of them plays a big role in South Africa.

A

Ancient DNA studies often target the petrous bone for genetic analysis, as it may contain better-preserved DNA due to its protective characteristics/ high density.

1) Strand breaks due to degradation by microorganisms (postmortem processes)
2) Oxidative lesions
3) Contamination with researchers DNA.

Factor in South Africa: The composition of the soil can influence DNA preservation. Acidic soils tend to degrade DNA more rapidly, while alkaline or neutral soils may provide better preservation conditions. In South Africa, the soil composition can vary, and regions with more acidic soils may pose challenges for DNA preservation. ?

24
Q

Which statement about Evolutionary Developmental Biology is/are correct?
a) Evolutionary reversal of complex characters is not possible
b) Hox genes are involved in body patterning are present in all metazoa
c) Changes in gene expression result only from mutations in the promotor or the enhancer of the same gene
d) Ubx genes can drastically change adult morphology in a single mutational step

A

a) Incorrect. Evolutionary reversal of complex characters is indeed possible. While it may be challenging and might not occur frequently, certain traits can be lost and then re-evolve in different lineages.

b) Correct. Hox genes play a crucial role in body patterning during development, and they are found in the genomes of a wide range of metazoan organisms.

c) Incorrect. Changes in gene expression can result from various mechanisms, including mutations in promoters, enhancers, transcription factors, chromatin structure, and post-transcriptional regulation.

d) correct. Ultrabithorax (Ubx) genes are known to play a role in the development of segment identity in insects. While a single mutational step might have significant effects, the extent of the morphological change would depend on the specific mutation and its impact on the developmental pathways.