Altfragen Flashcards

1
Q

Zusammensetzung Luft

A

78 Stickstoffs
20 Sauerstoff
0,04 CO2

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2
Q

Floral dip

A

Agrbacteriem mit Plasmid in Lösung
Blüte rein
Mutagenese
Samen -> Seletivmedium

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3
Q

CRISPR

A

Clustered regularly interspaced short palindromic repeats
crRNA: spacer und repeat, bindet an DNA und Cas9
PAM Motiv: NGG in Zeil DNA notwendig
tracrRNA: paart mit crRNA

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4
Q

Nitratreduktase

A

Hauptregulationsschritt der Nitrat-Assimilation
Homodomer
schreittweise Elektronenübertragung: NADH > FAD > Cytochrom > Mo-Cofaktor

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5
Q

Nitritreduktase

A

Elektronen aus Ferredoxin > FeS-> Sirohäm Cofaktor
Monomer
lichtabhängig

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6
Q

Regulation Nitratreduktase

A

transkriptionell: langsam
posttranskriptionell: schnell
Regulation durch Rotlicht (Phytochrom) -> im Dunkeln kein reaktives Nitrit

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7
Q

Asssimilation

A

Einbau anorganischer Stoffe in orgenaische Verbindungen

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8
Q

Ferredoxin

vorhanden in

A

Glutamat Synthase
Nitritreduktase
zyklischer Elektronentransport
Thioredoxin-System
DAHP Synthase I in Shikimatweg
APS-Reduktase (Sulfat - Sulfit)

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