Alternate and emerging technologies

Question 1

For Gel Technology: Microtubes for ABO reverse typing use _____

Answer 1

buffered gel

Question 2

what are the advantages of Gel technology?

Answer 2

• No wash step
• No antiglobulin control cells
• decreased sample volume
• More reproducible
• Less variability
• More sensitive and Specific

Question 3

what is the gel in the gel test made of?

Answer 3

dextran-acrylamide containing predespensed reagents

Question 4

agglutinated cells form a cell layer at the top of the gel media is a _____

Answer 4

4+

Question 5

all cells pass through the gel and settle on the bottom of the micro tube

Answer 5

negative

Question 6

agglutinated cells dorm a layer at the top of gel media and unagglutinated cells form a layer at the bottom of the microtube

Answer 6

mixed field

Question 7

what are the major disadvantages of gel technology?

Answer 7

• sample restrictions

- special equipment

Question 8

what are the sample restrictions of gel technology?

Answer 8

• No hemolyzed, icteric or lipemic samples

- No rouleaux

Question 9

what are the 3 solid-phase technologies?

Answer 9

• SPRCA
• Solid-Phase Protein A
• Solid-Phase enzyme- linked Immunosorbent assay (ELISA)

Question 10

what is the major difference between first gen and second gen SPRCA tests?

Answer 10

first gen: target antigen is added to microplate by user

second gen: target antigen is added by manufacturer

Question 11

what does a positive SPRCA look like?

Answer 11

adherence of indicator RBC’s to part or all of well bottom depending on rxn strength.

Question 12

is a component of the cell wall of Staphylococcus aureus that has a very high affinity for the ___ portion of most immunoglobulin classes

Answer 12

• protein A

- Fc portion

Question 13

assay to test for antibodies to platelet glycoproteins

Answer 13

ELISA

Question 14

what is the solid-phase ELISA used primarily for?

Answer 14

compatibility testing