Alp Flashcards
substrate of bessey lowry and brock
P-nitrophenyl phosphate
Substrate for king-armstrong
Phenyl phosphate
Substrate for sinowara-jones-reinhart
Beta-glycerophosphate
Susbtrate for huggins and talalay
Phenolphthalein diphosphate
Substrate for moss
Alpha Naphthol phosphate
Susbtrate for klein, bobson and read
Buffered phenolphthalein
Product for bessey lowry and brock
P-nitrophenol
Yellow compound
P-nitrophenol
Product of huggins and talalay
Phenolphthalein
Red compound
Phenolphthalein
Require longer incubation
Sinowara-jones-reinhart
Require protein removal
King-armstrong
Cause false increase in alp det
Lipemic
Icteric
Hemolysis
Hemoglobin (>100mg/dL)
Bilirubin (>20mg/dL)
Clot exposure (20-30%)
Cause false decrease in alp det
EDTA
Citrate
Oxalate
Absorbance reading
Increasing or decreasing?
How many reading?
Multiply by??
Increasing
2 reading
2187
Reference value of Alp
35-123 iu/L
ph of the reaction
pH 10.3
Wavelength of reaction
405 nm