Agilent HPLC Flashcards
What is the primary function of the Agilent 1200 series?
The primary function of the Agilent 1200 series is to perform high-performance liquid chromatography (HPLC).
True or False: The Agilent 1200 includes a built-in data analysis software.
True
What component of the Agilent 1200 is responsible for solvent delivery?
The pump is responsible for solvent delivery in the Agilent 1200.
Fill in the blank: The Agilent 1200 series is known for its __________ performance.
high-resolution
What type of detectors can be used with the Agilent 1200?
UV-Vis detectors and fluorescence detectors can be used with the Agilent 1200.
What is the role of the autosampler in the Agilent 1200?
The autosampler automatically injects samples into the HPLC system.
Multiple choice: Which of the following is a feature of the Agilent 1200 series? A) Manual sample injection B) Manual data recording C) Automated sample handling D) None of the above
C) Automated sample handling
What is the maximum pressure rating of the Agilent 1200 pumps?
The maximum pressure rating is typically up to 600 bar.
True or False: The Agilent 1200 series can be used for both analytical and preparative applications.
True
What type of chromatography can be performed with the Agilent 1200?
The Agilent 1200 can perform reversed-phase chromatography, among others.
Fill in the blank: The __________ is used to separate components in a mixture in the Agilent 1200.
column
What is the purpose of the online degasser in the Agilent 1200?
The online degasser removes dissolved gases from the solvents, improving the performance of the system.
Multiple choice: Which component is NOT part of the Agilent 1200 series? A) Pump B) Autosampler C) Gas Chromatograph D) Detector
C) Gas Chromatograph
What is the significance of the Agilent ChemStation software?
The Agilent ChemStation software is used for data acquisition and analysis in HPLC.
True or False: The Agilent 1200 can operate in both isocratic and gradient modes.
True
What is the main advantage of using the Agilent 1200 series in laboratories?
The main advantage is its high sensitivity and reproducibility in analytical results.
Fill in the blank: The __________ of the Agilent 1200 is crucial for maintaining consistent flow rates.
pump
What is the typical flow rate range for the Agilent 1200 series?
The typical flow rate range is from 0.1 to 5.0 mL/min.
Multiple choice: Which type of sample injection method is commonly used in the Agilent 1200? A) Manual B) Automated C) Both A and B D) None of the above
C) Both A and B
What is the purpose of the column thermostat in the Agilent 1200?
The column thermostat maintains a constant temperature for better separation performance.
True or False: The Agilent 1200 can only be used with Agilent brand columns.
False
What type of maintenance is recommended for the Agilent 1200 system?
Regular cleaning and inspection of the pump, detector, and autosampler are recommended.
Fill in the blank: The __________ in the Agilent 1200 is responsible for detecting the separated compounds.
detector
What is the benefit of using a fluorescence detector with the Agilent 1200?
Fluorescence detectors provide higher sensitivity for specific analytes.
