Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Immuno lab > Agglutination > Flashcards

Agglutination Flashcards

1
Q

• It is actually a foreign substance

A

Antigen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

• Substance that is produced against the antigen

A

Antibody

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

• particulate antigens are linked together with its corresponding
antibodies

A

Agglutination

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

• Used as indicator cells

A

Red cell suspension

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

• Demonstrates Ag-Ab reactions

A

Red cell suspension

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

soluble which is difficult to see

A

Ag-Ab reaction

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Specific specimen of the RCS

A

Anti-coagulated blood
(purple=EDTA=anticoagulant)
Packed RBC(after centrifugation)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Preparation of Red Cell Suspension

A

Wash 3x for 5 mins 1500 rpm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What does washing remove

A

Cytokines
Unwanted antibodies that may interfere with antigen-antibody reaction.
Micro-aggregates,
Plasma

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What will the antibodies that are still present cause?

A

Premature reaction rxn

False negative rxn

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q
  • An isotonic solution
  • Preserves the morphology of RBCs
  • Preferred because of economical advantage
A

NSS (0.9% Normal Saline Solution)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Other suspending agents

A

PBS (Phosphate Buffer Saline)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Ideal RCS

A

• 2 to 5 % Red Cell Suspension

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Vital

A

Thin suspension

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

For microtiter plates

A

reduce the concentration of RCS

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

not a good indicator cell

A

Thick suspension

17
Q

First step:

A

Add 2 drops of whole blood to the wasserman

18
Q

Second step

A

Wash the Cells 3x with 0.85% NSS

19
Q

Third step

A

Add NSS until the suspension will

become CHERRY RED color

20
Q

RCS should always be made fresh (using freshly extracted blood)

A

RCS should always be made fresh (using freshly extracted blood)

21
Q

Shelf life of RCS

A

Used within 24 hours

22
Q

Preservatives to be used:

A

Glycerol

23
Q

Process of progressively reducing or lowering the concentration of
solute in a given solution

A

Serial Dilution

24
Q

Measures the antibody concentrations in test serum

A

Serial Dilution

25
Q

High titer

A

high infection/high immunity

26
Q

Varying antigen

Volume of antibody is constant

A

Alpha

procedure (Dean Webb procedure)

27
Q

Varying antibody

Volume of antigen is constant

A

Beta procedure (Ramon procedure)

28
Q

Excess of antibody/extremely high titer reacting to small amount of antigen
HIGH ANTIBODY

A

Prozone reaction

29
Q

Excess of antigen/low titer reacting to a very small amount of antibody
LOW antibody

A

Post zone reaction

30
Q

Primary Phenomenon

A

Sensitization

Binding of Ab to Ag

31
Q

Secondary Phenomenon

A

Lattice formation

2 Fab

32
Q

Tertiary Phenomenon

A

Detected by effect on tissues or cells

33
Q

DF

A

TV/SOLUTE

Immuno lab (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions