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Preanalytical Procedures > Agars > Flashcards

Agars Flashcards

1
Q

Bile Esculin Agar (BEA)

A

Used to ID group D streptococci and enterococci. Hydrolysis of esculin by group D strep leaves a brown color to the medium.

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2
Q

Bile esculin azide agar with vancomycin

A

For cultivation of vancomycin-resistant enterococci. Azide inhibits GN bacteria and vancomycin will inhibit susceptible GP bacteria

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3
Q

Blood Agar (BA)

A

Used to grow nonfastidious microorganisms and determine hemolytic reactions

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4
Q

Bordet-Gengou Agar

A

For isolation of Bordetella pertussis and Bordetella parapertussis. Methicillin is used to inhibit growth of other organisms

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5
Q

Brain-heart infusion agar or broth

A

Used to grow fastidious organisms

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6
Q

Buffered charcoal-yeast extract agar (BCYE)

A

Enrichment for Legionella spp. and supports growth of Francisella and Nocardia spp

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7
Q

Buffered charcoal-yeast extract agar with antibiotics

A

Selects for Legionella spp

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8
Q

Burkholderia cepacia selective agar

A

For recovery of B. cepacia from cystic fibrosis patients

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9
Q

Campy-blood agar

A

Selective for Campylobacter spp. Contains vancomycin, trimethoprim, polymyxin, amphotericin B, and cephalothin

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10
Q

Campylobacter thioglycollate broth

A

Selective for recovery of Campylobacter spp. incubated at 4C for cold-enrichment

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11
Q

CDC anaerobe 5% sheep blood agar

A

Improved growth of obligate, slow-growing anaerobes

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12
Q

Cefoperazone, vancomycin, amphotericin (CVA) agar

A

Selective medium for isolation of Campylobacter spp.

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13
Q

Cefsulodin-irgasan-novobiocin (CIN) agar

A

Selective for Yersinia spp; can isolate Aeromonas spp

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14
Q

Chocolate Agar

A

Cultivation of fastidious microorganisms such as Haemophilus spp., Brucella spp., and pathogenic Neisseria spp

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15
Q

Chromogenic media

A

Designed to optimize growth and differentiate a specific type of organism; Routinely used for MRSA

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16
Q

Columbia Colistin-nalidixic acid (CNA) agar

A

Selective isolation of GPC

17
Q

Cystine-tellurite blood agar

A

Isolation of Corynebacterium diphtheriae (black colonies)

18
Q

Eosin methylene blue (EMB) agar

A

Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli; eosin and methylene blue are inhibitors/ pH indicators

19
Q

Gram-negative broth

A

Selective liquid medium for enteric pathogens

20
Q

Hektoen enteric (HE) agar

A

Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp., from other enteric GNRs; contains bile as an inhibitor and bromthymol blue as a pH indicator

21
Q

Loeffler medium

A

Isolation and growth of Corynebacterium spp.

22
Q

MacConkey agar

A

Isolation and differentiation of lactose-fermenting and non-lactose-fermenting enteric bacilli; contains bile and crystal violet as an inhibitor and used neutral red as a pH indicator

23
Q

MacConkey sorbitol agar

A

For selection and differentiation of E. coli O157:H7 in stool specimen; contains sorbitol (O157 cannot ferment this sugar while other E. coli strains can)

24
Q

Mannitol salt agar

A

Selective differentiation of staphylococci

25
Q

New York City (NYC) agar

A

Selective for Neisseria gonorrhoeae; also supports growth of Ureaplasma urealyticum and some Mycoplasma spp.

26
Q

Phenylethyl alcohol (PEA) agar

A

Selective isolation of aerobic GPC and GPR and anaerobic GPC and GNR

27
Q

Reagan Lowe

A

Enrichment and selective medium for isolation of Bordetella pertussis

28
Q

Salmonella-Shigella (SS) agar

A

Selective for Salmonella and some Shigella spp.; contains bile, sodium citrate, and brilliant green as inhibitors and neutral red as a pH indicator

29
Q

Schaedler agar

A

Nonselective medium for the recovery of anaerobes and aerobes; Selective for Campylobacter spp. and Helicobacter spp.

30
Q

Selenite broth

A

Enrichment of isolation of Salmonella spp.

31
Q

Skirrow agar

A

Selective for Campylobacter spp

32
Q

Streptococcal Selective agar (SSA)

A

Selective for Streptococcis pyogenes and Streptococcus agalactiae

33
Q

Tetrathionate broth

A

Selective for Salmonella and Shigella spp., except Salmonella enterica Typhi

34
Q

Thayer-Martin agar (TM)/ Modified Thayer Martin (MTM)

A

Selective for N. gonorrhoeae and N. meningitidis; Supports the growth of Francisella and Brucella spp

35
Q

Thioglycollate broth

A

Supports growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms

36
Q

Thiosulfate citrate-bile salts (TCBS) agar

A

Selective and differential for Vibrio spp

37
Q

Todd-Hewitt broth supplemented with antibiotics (LIM)

A

Selection and enrichment for Streptococcus agalactiae in female genital specimens

38
Q

Trypticase soy broth (TSB)

A

Enrichment broth used for subculturing various bacteria from primary agar plates

39
Q

Xylose lysine deoxycholate (XLD) agar

A

Isolation and differentiation of Salmonella and Shigella spp. from other enteric GNRs; contains bile as an inhibitor and used phenol red as a pH indicator

Preanalytical Procedures (4 decks)

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