Agappe Flashcards

1
Q

Secondary source of energy
Proteins from the major portion of dissolved substances

A

Total protein

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2
Q

Colorimetric deterioration of total protein based on the principle of the …

A

Biuret reaction

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3
Q

Protein in plasma/serum sample forms a what color?

A

Blue colored complex

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4
Q

Total protein reagent

A. Potassium iodide
B. Potassium sodium tartarate
C. Copper sulphate
D. Sodium Hydroxide

A

A. 6 mmol/L
B. 21 mmol/L
C. 6 mmol/L
D. 58 mmol/L

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5
Q

Open vial stability

A

20 days

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6
Q

On board calibration stability

A

7 days

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7
Q

Sample

A

Serum
Plasma (free of hemolysis)

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8
Q

Interferences

A. Ascorbic acid
B. Hemoglobin
C. Bilirubin

A

A. 500 mg/dL
B. 1,000 mg/dL
C. 20 mg/dL

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9
Q

Wavelength

A

546 nm

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10
Q

Temperature

A

37°C

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11
Q

Standard conc.

A

6 g/dL

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12
Q

Linearity

A

15 g/dL

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13
Q

Incubation

A

10min

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14
Q

A. Sample volume
B. Reagent volume

A

A. 20 uL
B. 1,000 uL

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15
Q

Reference range

Serum

A. Newborn
B. 1wk
C. 7mon-1yr
D. 1-2 yr
E. more than 2yr

A

A. 4.6-7 g/dL
B. 4.4-7.6 g/dL
C. 5.1-7.3 g/dL
D. 5.6-7.5 g/dL
E. 6-8 g/dL

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16
Q

Increased levels

A

Dehydration
Myeloma

17
Q

Decreased levels

A

Liver disorders
Nephrotic syndrome
Malnutrition
Haemorrhage