Agappe Flashcards
A. Major carbohydrate present in the blood
Serves as primary source of energy
B. Obtained from ingested?
A. Glucose
B. Starch and Sugar
Glucose reagent R1
Tris buffer (pH 7.40)
92 mmol/L
Glucose reagent R1
Phenol
-0.3 mmol/L
Glucose reagent R1
Glucose oxidase
-1,500 U/L
Glucose reagent R1
4-Aminophenazone
2.6 mmol/L
Glucose standard
Concentration:
-100 mg/dL
Glucose
A. Reagent stability
B. Reagent stored
A. up to expiry date
B. 2-8 °C
Open vial stability
4wks @ 2-8°C
On-board calibration stability
20 days
Reagent Deterioration
Turbidity/precipitation
Sample
Serum
Plasma (free of hemolysis)
CSF
Interferences:
A. Bilirubin
B. Hemoglobin
A. 20 mg/dL
B. 1,000 mg/dL
A. Mode of reaction
B. Slope of reaction
A. Endpoint
B. Increasing
Wavelength
505 nm
Temperature
37°C
Standard concentration
100 mg/dL
Linearity
600 mg/dL
Incubation time
10mins
A. sample volume
B. reagent volume
A. 10 uL
B. 1,000 uL
Reference range
Serum/Plasma
Newborn
A. 1 day
B. more than 1 day
A. 40-60 mg/dL
B. 50-80 mg/dL
Serum/Plasma
Child
60-100 mg/dL
Serum/Plasma
A. Adult
B. 60yrs
C. 90yrs
A. 74-100 mg/dL
B. 82-115 mg/dL
C. 75-121 mg/dL
WB
Adult
65-95 mg/dL
CSF
A. Infant
B. Adult
A. 60-80 mg/dL
B. 40-70 mg/dL
Urine
1-15 mg/dL
U(24hrs)
<0.5 gram/day
Elevated blood glucose levels
DM
Hyperthyroidism
Hyperadrenalism
Decreased levels
Insulinoma
Hypothyroidism
Hypopituitarism
Excessive glucose stored as? Mainly?
Inactive glycogen; liver and little in muscles
Glucose conc. normally maintained at what level?
Constant level