Advances in EM

Question 1

Main issue and other issues of CryoEM

Answer 1

Poor signal to noise

Unstable sample stage, beam induced movement, electon beam damages crystal(gas formation)

Question 2

Biggest Advancemet

Answer 2

Direct Electon detectors-
Takes very fast snapshots
no coversion of electron to photons(no error)
programms developped for motion correction and movie processing

Question 3

Other main one

Answer 3

Phase plates- that improve contrast- can decrease resolution by inelastic scattering of electrons by phase plate material

Question 4

Smaller advancements that contributes

Answer 4

Energy filters- filters out inelastically scattered electron
More powerfull microscopes
More stable sample stages
Faster computing power for algorithms
Automation of sample prep- better reproducability

Question 5

How is CryoEM tomography helped

Answer 5

Direct detection and phase plate-reduces contrast- can identify orientations better and classify images better- increase small classes- so tey arent disregarded

Powerful classification algoritms are being produce to take advantage of the increase in computing power