Advanced Evidence Types Flashcards

1
Q

What are the 7 kinds of forensic geology?

What can be said about 3/7 of these?

A

rocks
sediments
soils
dusts
minerals
fossils
anthropogenic e.g. concrete, bricks

sediments soils and dust are mixtures of organic/inorganic particles where
- organic fractions (hydrocarbon based, plant/animal based) derive from: biological processes, e.g. shells and forams (calcite, aragonite), phytoliths (silica), diatoms, or CHO based products (e.g. coal)
- inorganic fractions include rock fragments, mineral grains and amorphous matter, e.g. volcanic glass

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2
Q

When are rocks encountered in forensic casework?

A
  • weights to sink bodies
  • in bags containing illicit substances/firearms
  • as concealment material in smuggling cases
  • as projectiles (thrown or catapulted
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3
Q

What are three main classifications of rocks?

Which one is most interested in as forensic perspective?

A
  • igneous - rocks formed from liquid magma cooled deep in the earth or extruded onto surface by volcanos
  • metamorphic - rocks formed by varying degrees of heat and pressure on any type of existing rock
  • sedimentary - rocks formed by accumulation of sediment in water and air by processes of erosion, precipitation, evaporation and compaction
  • sedimentary - around 70 % of earths surface
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4
Q

What is diatomaceous earth?

A
  • predominantly made of 80-90 % diatoms (silica) aluminium oxide if forms of clay minerals, iron oxide
  • diatoms are useful form of TE
  • if diatomaceous earth in rocks, got something useful to look at
  • it is in food, medicines, cosmetics, absorbents, insulating materials, pet litter, even as a stabilising component in dynamite – chances of finding it are pretty high
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5
Q

What are sediments and what makes them different to rocks?

What are four main categories of sediment?

A
  • particle mixtures from a variety of sources, transported and deposited by air, water or ice
  • this makes sediments distinct from rock
  • gravel
  • sand
  • silt
  • clay
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6
Q

Define gravel
- when encountered
- size discrimination
- rounded gravel

A
  • encountered in casework in tyre treads, shoe soles, in drowning victim’s mouths/respiratory tract/clothing (in pockets/hoods/shoes), or on associated adhesive tapes (gagged or bound)
  • size discrimination:
  • gravel = 2 mm – 64 mm
  • cobbles = 64 mm – 256 mm
  • boulders = >256 mm
  • shingle = rounded gravel
  • found in driveways, flowerbeds, decorative reasons
  • also found in marine and lake environments because water is constantly eroding surface
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7
Q

Define sand
- when encountered
- size discrimination
- for a sediment to be classed as sand…
- what is it made up of

A
  • encountered in casework involving beaches, sand dunes, and construction sites;
  • found on/in footwear, clothing, vehicles, bodies, washing machine filters and U-bends of sinks and toilets (because it sediments)
  • when flush toilet or wash garments with sand – particles will be trapped
  • size discrimination:
  • sand grains = 0.063 mm – 2 mm
  • for a sediment to be classed as sand it must contain >50% sand grain sized particles otherwise gravel or something else
  • made up of many different minerals – rock fragments (lithic grains) – predominantly quartz
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8
Q

Define mud
- smallest two constituents
- size discrimination
- definition
- encountered when
- what else do samples often contain

A
  • encompasses silt and clay (smallest sediments) with some sand and gravel
  • mud particles typically = <0.063 mm
  • mud is any sediment that has a sticky character when wet so good form of TE
  • clings to stuff – big tick for transfer thinking about background transfer, persistence
  • chances of finding in UK is high
  • encountered frequently in casework on skin, under fingernails, on clothing/footwear, in/on vehicles; from coastal/river floodplains, fields, and woodlands
  • many samples also contain organic matter (grass, weeds, pollen)
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9
Q

define soil
- definition
- homogeneous/heterogeneous
- how is it found on earths crust
- how are they dictated
- what is it made up of

A
  • soil is unconsolidated stable material on the Earth’s surface (it is loose (isn’t a lump) and it is on the surface rather than inside the earth); plant growing material (it is biologically active – good medium)
  • extremely heterogeneous so good form of TE as is highly variable
  • but need to take care when using microscopic techniques to understand chemical variety in sample (cannot test one area and assume representative of whole sample)
  • soil is present on earth’s crust in distinct layers - horizons
  • soils are dictated by how they are formed or where they are found:
  • climate (arid, hot cold, wet, or dry)
  • parent material (what type of rock)
  • age (older soil = more fertile and denser, because longer it has had to form)
  • biological activity
  • topography determine soil type formation
  • soil is made up of:
  • non-living: inorganic minerals, salt crystals, decaying plant/animal matter (humus), shells, insect exoskeletons, fossils, bones, and teeth
  • living: bacteria, algae, fungi, plant roots, invertebrates, small mammals
  • unusual: cement, plaster, metallic fragments, glass, paint flakes, paper, fibres, plastic fragments
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10
Q

define dirt and grime

A

dirt - combination of dust and soil
grime - wet dirt

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11
Q

Why is soil a good form of TE?

A
  • good form of TE as can work out where it is from using UK soil observatory database by British geological survey
  • database that lets you search almost any part of UK by any criteria you want
  • can put in anything found from analysing trace of soil and will tell where likely find that exact makeup/composition of soil
  • unusual components of soil (cement, plaster, metallic fragments, glass, paint flakes, paper, fibres, plastic fragments) are useful in TE too
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12
Q

Define dust (size, size repercussion, chemical compositions)

A
  • particles generally <100 μm
  • if <10 μm, can be transported thousands of miles
  • this size has big repercussion when trying to determine background, persistence, activity level
  • need to bear in mind that size of dust particle may mean it didn’t originate there – might have been carried in wind
  • chemical composition varies considerably and is indicative of geographical area or type of place it came from
  • natural, industrial (will be different might have soot from chimneys), household (predominantly skin cells), street (particles from brake dust/exhaust fumes), water sources, etc.
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13
Q

define minerals
- definition
- most abundant category
- phyllosilicate class
- also known as
- define clay

A
  • another geological form of TE
  • silicate minerals
  • phyllosilicate class most interesting
  • comprised of sheets of repeating SiO4 tetrahedra - gives minerals from this class a crepe-y appearance and allows them to be fractured easily
  • also known as clay minerals
  • clay - geological sediment (< 2 um)
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14
Q

Where do we find different types of minerals
- vermiculite
- muscovite (natural mica)
- kaolinite (china clay)
- magnesium silicate (talc)
- bentonite
- hectorite
- Illite (French green clay)

A
  • vermiculite – grey mineral
  • bit like diatomaceous earth – it is everywhere
  • come in different sizes – have different properties
  • found anywhere you want insulating or fire retardant properties
  • used as the insulating material in fire-resistant safes, chimneys, brake-linings, floor screed, concrete, fire-proof walls
  • highly likely to be encountered as a form of trace especially if someone has tried to break into a safe and burgle it – may end up with traces of vermiculite on clothing, tools, shoes and under fingernails – might think it is just dues
  • muscovite (natural mica)
  • has repeating tetrahedral sheets – good mineral to use in makeup as soft and shiny
  • kaolinite (china clay)
  • in clay face masks
  • magnesium silicate (talc)
  • talcum powder
  • bentonite
  • face masks and mascara
  • hectorite
  • face masks and mascara
  • Illite (French green clay)
  • face masks
  • good for angry and red skin – soothing and calming properties
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15
Q

What are gemstones?

Where are these likely to be found?

A
  • another form of minerals
  • these are minerals that have been cut and polished to be used in jewellery or as ornaments
  • not likely that you are going to find gemstones as a trace material for example in tread of someone’s boots but might find in forensic gemology
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16
Q

What does forensic gemology consist of?

How can forensic gemmologist tell difference between various gemstones?

A
  • counterfeits and simulant gemstones
  • diamonds cost a lot, they can be simulated by a lot of other minerals but it takes an expert to differentiate them
  • ways in which forensic gemmologist can tell differences between various gemstones and analyse them to give their expert opinion to say if something is genuine of fake
  • luminescence, fluorescence (when irradiated with UV light, they emit visible light
  • phosphorescent - if the luminescence continues after the exposure to UV radiation. this happens due to impurities and defect in the crystal lattice
  • birefringence (most significant optical property used for the identification of gemstones)
  • refraction, dispersion, pleochroism
  • hardness, specific gravity, magnetism, piezoelectricity (electrical activity in mineral when apply pressure), pyroelectricity (electrical activity in mineral when apply heat), thermal conductivity (does it conduct heat as mineral)
17
Q

What does XRD allow for in forensic gemology?

A
  • able to identify impurities and defects in the crystal lattice of gemstones
18
Q

What is included in the optical analysis of isotropic substances?

What is an isotropic substance and give two examples

How does this work with Snell’s law?

A
  • measure the RI
  • if an isotropic substance is illuminated with normal incidence to the crystal face, all light passes through without deviation
  • diamond which have a cubic structure
  • opals (hydrated silica) which are amorphous
  • Snell’s law still applies as sin0 = 0
  • velocity changes but not the direction of light
19
Q

What is a uniaxial substance and give two examples

What happens when light hits a uniaxial substance

What is included in the optical analysis of uniaxial substances?

A
  • tetragonal or hexagonal crystals
  • light incident on the cleavage face of the crystal will break into two separate rays as the light travels through the crystal
  • the waves of the two rays vibrate in planes perpendicular to each other and travel through the crystal at different velocities
  • the faster ray emerges from the crystal slightly ahead and in a different location to the slower ray
  • the two rays are known as the ordinary ray (O) and the extraordinary ray (E)
  • the two rays vibrate in different planes through the material and have different RIs
  • birefringence is the difference between these two refractive indices
  • as the polarisation of light changes through these materials, this can be measured
20
Q

What does anthropogenic mean?

Give examples of anthropogenic materials?

A
  • means manmade
  • some are not fully manmade because they started life as natural minerals but we have done something to them to make manmade
  • concrete - mixture of aggregate and cement
  • cement is derived from natural minerals but we have processed them
  • aggregate is just gravel stones
  • bricks, roof tiles, pipes, pottery
  • all derived from natural clays but fired in a kiln - been changed
  • glazed ceramics
  • can date these as they are made in factory where records are kept and sometimes they have manufacturer stamp on bottom
  • plastic fragments
  • fibres
  • paint flakes
  • glass fragments
  • metal fragments
21
Q

What are recovery considerations for soils? (suspected contact points, footwear marks, packaging, sketch/photograph)

A
  • take control sample from suspected contact points
  • using a clean trowel sample surface to which contact has been made (normally scraping the surface is sufficient as surface soil is to a depth no deeper than 16 cm)
  • if contact is suspected below 16 cm then contact lab
  • where footwear mark is also recovered, leave any samples adhering to cast in place but also take samples from surrounding areas
  • package each sample separately in properly sealed, heavy duty paper bag
  • consider placing samples in a tamper evident bag and freezing to reduce the likelihood of yeast of fungal growth
  • include a sketch/photograph showing areas sampled
22
Q

What is analytical workflow for soil/geology analysis?

A
  • gross examination, recovery, and collection
  • preliminary evaluation of physical characteristics

(physical fit assessment – most probative value but unlikely to get this – not going to get a piece of rock and say it fits exactly into other bit of rock. this is even less likely with soil as it is unconsolidated – it doesn’t have form)

  • all microscopic techniques – PLM
  • microspectrophotometry – colour determination
  • measurement of pH and electrical conductivity (soil pH – indicates where it is from as per geological survey maps, if soil is acid or alkaline)
  • infrared spectroscopy – organic content
  • raman spectroscopy – inorganic and carbon content
  • SEM-EDX – SE mode for surface topology & BSE mode for homogeneity
  • XRD – crystal structure, polymorphs
  • AAS, XRF, NAA, ICP-AES – elemental composition
  • isotopic analysis (isotope ratio)
  • MVA – most widely used = HCA and PCA (hierarchical and principal component analysis)
  • get lots of data from analysing soil residue so MVA good for this
23
Q

What physical characteristics should be observed in soil/geology analysis?

A
  • weight and volume
  • colour and texture
  • Munsell soil/rock colour chart
  • for individual particles:
  • dimensions, surface area, perimeter, and shape (form, sphericity, angularity (how angled or sharp it is), irregularity (how non-shape like it is, is there any protrusions, is it branched)
24
Q

How can metals be used as a form of TE?

A
  • rust and metal shavings transferred to a persons clothing
  • metal filings on a pipe wrench after a twist attack on a doorknob
  • metal weapon used in an assault leaving trace in the wound
  • metal from a crowbar used to force open a window or door
  • gunshot residue
  • explosives residue
  • fireworks and sparklers
  • lightbulb filaments
25
Q

What are primary techniques used in the analysis of metal trace evidence?

A
  • SEM-EDX
  • XRF
  • XRD
  • HPLC and GC-MS
  • AAS
  • LA-ICP-MS
  • colour spot and presumptive tests also regularly used (particularly for GSR)
26
Q

What is difference between two types of GSR?

A

inorganic:
- mainly from primer mixture, some from cartridge case
- historically heavy metal components, although toxic metal free primer more common now

organic:
- mainly from smokeless powder, composed of primary explosives, stabilisers, plasticisers, sensitisers, and flash inhibitors
- combustion products also found on spent cartridge and firearm muzzle

27
Q

What are four categories of GSR detection?

A

1 - optical methods:
- organic compounds identified due to strong IR-luminescence
- methods non-destructive, rapid but low specificity and high LoD

2 - Chemographic testing:
- invasive but can be performed at crime scene
- most target inorganic elements with presumptive test
- high selectivity but only qualitative with risk of false positives

3 - spectrometry:
- primarily identify inorganic components
- SEM-EDX most common as it is quantitative, gives highest compositional and morphological detail, but takes time

4 - separation methods:
- particularly coupled with mass spectrometry e.g. LC/GC-MS/MS
- invasive method but reliable, specific and has a low LoD

28
Q

How can metals be detected in latent fingerprints?

A
  • of interest to be able to link activity with specific metal objects e.g. guns or explosives
  • XRF microscopy could enable this
  • needs large synchrotron to access this data with a high enough spacial and spectrographic resolution
  • able to identify differences between handling gun barrel and ammunition cartridge background activity
29
Q

How do we detect explosive trace evidence?

What is good about this technique?

What other techniques are available?

A
  • currently use derivatives of ion mobility spectrometry (separates and identifies ionised molecules present in the gas phase based on their mobility in a carrier buffer gas)
  • builds on the concepts of mass spectrometry - controlled ionisation and separation based on an ions interaction with an electric field to enable detection and identification
  • measurements can occur in milliseconds, which makes it great for usage in situations where fast, reliable results are required e.g. airports
  • techniques including GC/LC, SEM, FTIR, Raman & other spectrometric methods used to elsewhere to analyse the organic and inorganic components
30
Q

Why are fireworks a great form of TE?

A
  • fireworks are a great combination of multiple TE types: paper, plastic, explosives, metals
  • all previous discussions of analytical approaches to these individual elements are of interest here
31
Q

How are sparklers used?

What can explosive analysis techniques do for sparklers?

What about with chemometrics?

A
  • sparklers are particularly interesting as commonly used to initiate inorganic homemade explosives
  • explosives analysis techniques used to great effect to detect and identify a sparkler, they aren’t enough to distinguish between brands of sparkler
  • combining ICP-MS to detect elemental profiles of 7 metal components (V, Co, Ni, Sr, Sn, Sb & W) with chemometric approaches to analyse the profiles has shown a discrimination with 100% match to sparkler manufacturer
32
Q

How can a lightbulb filament be used as trace evidence?

A
  • likely the lightbulb from a headlamp is going to be involved in analysis to answer:
  • were the headlights illuminated?
  • turning without indication?
  • brake lights not working?
  • as well as potentially linking at a hit-and-run or ram-raid
33
Q

What are three types of modern automotive lightbulbs?

A

1 - halogen – most popular
- tungsten filament in glass tube with N/Ar
2 - LED – future challenge: range of semiconductors – small, metal doped
3 - xenon/high intensity discharge

34
Q

How can lightbulb tungsten filament analysis tell us if lamp was illuminated at time of impact?

A
  • tungsten is rigid and brittle at ambient temperatures and ductile at incandescent operating temperatures
  • if filament was off - no pronounced distortion
  • if filament was on - inertial force distortion on hot filament
  • can use a multimeter to check if it still works
35
Q

What is tattooing?

Why is the study of tattoos of interest to forensic science?

What can be subjective about tattoo analysis?

A
  • the practise of puncturing the skin and introducing pigment which is retained by macrophages in the dermis of the skin
  • the study of tattoos is of interest to forensic science for two main reasons:
    1 - identification
    2 - tracking movement of people and animals
  • the description of tattoos is open to interpretation
  • chemical analysis removes interpretation issues
36
Q

What are modern pigments made of?

Develop an analytical workflow?

A
  • organic pigments (Azos) or mineral pigments (TiO2, iron oxide)
  • all microscopic techniques – understand penetration depth and pigment distribution (just like with hair)
  • infrared spectroscopy – organic binders and pigments
  • raman spectroscopy – inorganic pigments
  • SEM-EDX and XRF – elemental composition of layers
  • pyrolysis-GC/MS and microchemical testing