Adiponectin paper (Boursereau et al. 2017) Flashcards
What were the authors investigating in this paper?
How do miRNAs relate to adiponectin’s anti-inflammatory action on skeletal muscle
What were the author’s objectives?
- Identify any miRNAs that are regulated by adiponectin in skeletal muscle
- Identify the functions of any identified mRNAs
- Determine if the same miRNAs found in mice are also regulated by adiponectin in humans
How did the authors address which miRNAs were regulated by adiponectin in skeletal muscle?
miRNA arrays to identify which ones were different between muscles that were producing adiponectin vs muscles that weren’t. Then they did RT-qPCR to address how much expression was changing
Why did they choose to focus on miR-711?
It was substantially upregulated in muscle cells with adiponectin
What experimental evidence allowed the authors to conclude that miR-711 was a major effector of the anti-inflammatory effects of adiponectin in skeletal muscle?
- miR-711 was substantially upregulated when adiponectin was being expressed, compared to the controls where the hormone wasn’t expressed
- miR-711 is also overexpressed when adiponectin is
- Blocking miR-711 negates any effects of adiponectin
- Expressing only miR-711, without any adiponectin, shows the same decrease on expression the TLR4 pathway components as what it seen with adiponectin treatment alone
How did the authors identify the function of miR-711?
In silico functional analysis. They looked for sequences in the genome that were complementary to miR-711’s sequence to determine which genes may be its targets
Why did the authors focus on components of the TLR4 pathway when determining if miR-711 was the effector?
These are involved in inflammatory signalling pathways, which was the author’s topic
How did the authors determine if miR-711 in mice also functions as adiponectin’s effector in human skeletal muscle?
RT-qPCR to determine expression levels in human myotube cells that were treated in vitro with adiponectin or nothing
