Actin Lab

Question 1

Immunoflorescence

Answer 1

Using colored proteins that bind to antibodies to create a certain color in a microscopic picture

Question 2

Actin Polymerization

Answer 2

G actin- globular (individual) binds with ATP and turns into –> dimer (two actin)
–> trimer (three) –> F actin (filament)

Actin filament binds from negative end to positive end and the ATP (phosphorus) glue falls out starting at the negative end

Question 3

Myosin and Cell Motility

Answer 3

• *Actin and Myosin complex moves organelles through the cells**
  1. organelle attaches to myosin which is attached to actin filament that acts as an internal highway for transportation throughout the cell

Question 4

Cytochalasin

Answer 4

Capping protein that inhibits filament production