Acid-Fast Stain

Question 1

What color will appear after staining

Answer 1

Hot pink

Question 2

What is an Acid-stain

Answer 2

differentiate cells based on presence or absence of MYCOLIC ACID

Question 3

What is MYCOLIC Acid

Answer 3

MYCOLIC acid are long fatty acid found in the cell wall with peptidoglycogen.
Waxy lipid.
Hidrophobic
Will not decolorize

Question 4

MYCOLIC acid 2 genera

Answer 4

Essential for the grow
1-mycobacterium Tuberculosi2
2- Nocardia most common manifestation Pneumonia

Question 5

Acid-fast Stain use

Answer 5

is not use as frequent as Gram stain because few stain content Mucous Acid

Question 6

Acid-fast stain procedure

Answer 6

x Heat fix bacteria Sample
x primary stain/mordan…. KYNIAN’S CARBOL FUCHSIN
x Decolorizer….acid alcohol
x Counterstain..Methylene Blue

Question 7

Endoespore Stain

Answer 7

Differential stain
Differentiate e.s. forming bacteria and non-e.s. for min bacteria
x Detects presence and location of E.S.

Question 8

Endospores characteristics

Answer 8

E. S. are a protective metabolically inactive structure
Does not play a role in reproduction
x are produce via sporulation.
x A vegetative cell (SPORANGIUM) produces the ES within itself.
x outer protein coat makes them resistant to heat radiation, chemical desinfection and desiccation.
xsurvival in hash conditions.
x no water the presence of DIPICOLONIC acid makes them resistant.

Question 9

Genera of ES

Answer 9

BACILLUS most are soil, freshwater, and marine saprophytes.
exp. anthrax
CLOSTRIDIUM most human intestine
tetanus
botulism
gas gangrene
p.coli