Acid base practical

Question 1

What is total protein?

Answer 1

Substances containing teo -CO-NH groups (i.e., peptide bonds) joined together directly or via a single carbon/nitrogen atom

Give a purple colour sith alkaline cooler solutions

Question 2

What is albumin?

Answer 2

At pH 4.2 it acts as a cation to bind anionic dyed

Question 3

Expand C¹V¹ = C²V²

Answer 3

C¹ = concentration of initial stock
V¹ = normally what we need, the amount of stock to be added to form a desired concentration
C² = final concentration
V² = final volume

Question 4

What is acidosis?

Answer 4

Low blood pH

Addition of H+ to bodily fluids

Question 5

What is alkalosis?

Answer 5

A rise in blood pH

Loss of H+ from bodily fluids

Question 6

What does a high pCO² and low pH indicate?

Answer 6

Respiratory acidosis

Question 7

What does a low pCO² and a high pH indicate?

Answer 7

Respiratory akalosis

Question 8

What does a low HCO³ and a low pH indicate?

Answer 8

Metabolic acidosis

Question 9

What does a high HCO³ and a high pH indicate?

Answer 9

Metabolic alkalosis

Question 10

How to correct absorbance for the dilution factor?

Answer 10

Add 1/

E.g., 1/0.02 dilution factor = 50

So times absorbance by 50

(If a fraction, flip the fraction)

Question 11

What does looking at HCO³ levels indicate?

Answer 11

Metabolic

Question 12

What does looking at levels of pCO² indicate?

Answer 12

Respiratory

Question 13

Which homeostatic mechanisms keep plasma pH in the normal range?

Answer 13

Renal system
Respiratory system