Acetamide Utilization

Question 1

What is the function (purpose) of acetamide utilization?

Answer 1

Differentiate microorganisms based on the ability to use acetamide as the sole source of carbon.

Question 2

What’s is the incubation period for the acetamide utilization?

Answer 2

Incubate aerobically at 35_37 c for up 4 days.
If slant may be reincubated for 2 additional days.

Question 3

Principle of acetamide utilization is ?

Answer 3

Bacteria capable of growth on this medium produce the enzyme acylamidase, Which deaminates acetamide to release ammonia.
The production of ammonia results in an alkaline PH , causing the medium to change color from green to royal blue.

Question 4

What is acetamide media made of?

Answer 4

Nacl(5g)
NH4H2po4(1g)
K2Hpo4 (1g)
Agar (15g)
Bromthymol blue indicator ( 0.8g)
Per 1000ml,acetamide(10g).
PH (6.8)

Question 5

Method of acetamide utilization:

Answer 5

1- inoculate acetamide slant with a needle using growth from an 18-24 hour cultur.
Don’t inoculate from a broth culture, Because the growth will be too heavy.

2- incubate aerobically at 35-37 c for up 4 days. If equivocal, the slant may be reincubated for 2 additional days.

Question 6

Expected results of acetamide are:

Answer 6

Positive: deamination of acetamide resulting in blue color

Negative: no color change

Question 7

Limitation of acetamide is:

Answer 7

Growth without a color change may indicate a positive test result. If further incubation results in no color change, repeat test with less inoculum .

Question 8

Quality control for acetamide:

Answer 8

Positive: pseudomonas aeruginosa -growth: blue color
Negative: Escherichia coli-
No growth : green color