Absorption by Roots Flashcards
Define plant physiology. (2 points)
- Plant physiology is the branch of biology which deals with the life functions or metabolism of plants.
- It includes the functioning of cells, tissues, organs, organ systems and the organism as a whole.
Give three names for the functions of the roots. Elaborate.
- Fixation
- Absorption
- Conduction
Name the 5 purposes of water inside the plant body.
- Being a constituent of the protoplasm
- Photosynthesis
- Transpiration
- Transportation
- Mechanical Stiffness
How is water used up in photosynthesis? (1 point only)
Water is used up in the green leaves as a raw material in the synthesis of glucose.
How is water used up in transpiration?
1 point
A large quantity of water gets evaporated as water vapour during transpiration, for cooling in hot weather, for producing a suction force, etc.
How is water used up in transportation?
Transportation of substances in water solution from the roots upward into the shoot (mineral salts) or from leaves to other parts (sugar, etc).
How does water help in maintaining mechanical stiffness?
Water provides turgidity (fully distended condition), which is necessary for the stiffness of plant tissues.
Name the forms in which minerals nutrients are absorbed from the soil by the roots.
- As salts
2. Simply as ions
Name the salts absorbed by the roots from the soil.
Nitrates, Phosphates, Sulphates, etc.
Name the ions absorbed by the roots from the soil.
Potassium, calcium, magnesium - (chlorophyll is made up of magnesium), chlorine, etc.
Name the functions of these mineral nutrients in the plant.
- They are required as constituents of cell and cell organelles.
- They are required in the synthesis of a variety of compounds or enzymes within the cell (chlorophyll is made up of magnesium).
Name the three characteristics on which the ability of plants to draw water from roots depends.
- A huge surface area provided by rootless and root hairs.
- Root hairs containing the solution (cell sap) at a concentration higher than that of the surrounding soil water.
- Root hairs having thin walls.
Explain the role of surface area of roots in absorption of water.
The surface area is increased by the presence of a thick bunch of rootlets (branch roots) and hundreds of root hairs.
Example - The small garden plant balsam has a thick bunch of rootlets and hundreds of root hairs.
Name the botanist who worked out that that the roots of any plant provide a huge surface area to facilitate the absorption of water.
Botanist - H.J. Dittmer (1937) Plant used - a four month old rye plant Aggregate root length - 600 km No. of root hairs - more than 14 billion Estimated total length of root hairs - exceeding 10,000 km
What is sap?
Water along with mineral salts absorbed by the roots
Root hairs are the extensions of?
outer (epidermal) cells of the root.
Root hairs contain large _ filled with a solution called _?
vacuoles, cell sap
Why does the cell sap have a higher concentration than that of surrounding water?
Due to dissolved salts.
Which characteristic of the root hair is important to draw water from the outside?
Having cell sap which has a higher concentration than that of the surrounding soil water.
This ‘higher concentration’ characteristic promotes which process?
Osmosis
Name the two outer layers of root hair.
Cell wall and cell membrane.
Name the characteristics of cell wall & consequently, what it allows.
The cell wall is thin and freely permeable. It allows the movement of water molecules and dissolved substances freely in and out of the cell.
Name the characteristics of cell membrane & consequently, what it allows.
The cell membrane is very thin, non-cutinised and semi-permeable, which means it allows water and molecules to pass through, but not the larger molecules of the dissolved salts.
In which characteristic does the secret of absorption of water from soil by roots lie?
In the cell membrane’s semi-permeability - it allows water molecules to pass through, but not the larger molecules of the dissolved salts.
Name the fine main phenomena, the result of which is the entire mechanism of absorption & conduction.
- Imbibition
- Diffusion
- Osmosis
- Active Transport
- Turgidity and Flaccidity
Define imbibition.
Imbibition is a phenomenon in which the living or dead plant cells absorb water by surface attraction.
Which substances have a strong affinity for water? Name the state of having this affinity.
Substances which are made up of cellulose (in cell wall) or proteins (starch) are hydrophilic (strong affinity for water).
What do hydrophilic substances do when in contact with moisture?
The imbibe water or moisture and swell up, e.g., dry seeds, wooden doors. swell up on contact with water or exposure to moist air.
Name two consequences of imbibitional pressure.
- Seed coat ruptures in case of germinating seeds.
2. It is an important force in the ascent of sap.
Define diffusion.
Diffusion is the free movement of molecules of a substance (solute or solvent, gas or liquid) from the region of their higher concentration to their lower concentration when the two are in direct contact.
Give an example of diffusion in plants.
Exchange of respiratory gases, transpiration
Give an example of diffusion in animals.
Exchange of respiratory gases in the alveoli
Name the requirements in the experiment demonstrating diffusion.
- Sugar cube/ crystal of potassium permanganate/ small tablet of a soluble dye
- Beaker
- Water
Give the steps of the experiment which demonstrates diffusion.
- Place a sugar cube or a small tablet of a soluble dry or a crystal of potassium permanganate in a beaker containing water, in one corner.
Describe what happens in the experiment demonstrating diffusion.
- The molecules of dye are more concentrated in and near the tablet of dye.
- Molecules move farther away to regions where they are less concentrated.
- This movement continues until molecules are uniformly distributed.
Name the step used to hasten the process of diffusion in the experiment.
- Stirring with a spoon or a glass rod hastens the process and you get a homogeneous solution much faster.
Define osmosis.
Osmosis is the movement of water molecules from their region of higher concentration (dilute solution or with a lower solute concentration) to their region of lower concentration (concentrated solution or with a higher solute concentration) through a semi-permeable membrane.
Define endosmosis
It is the inward diffusion of water through a semi-permeable membrane when the surrounding solution is less concentrated. This tends to swell up the cell.
What is the function of endosmosis?
It tends to swell up the cell.
Define exosmosis.
It is the outward diffusion of water through a semi-permeable membrane when the surrounding solution is more concentrated. Tends to cause shrinkage of cell.
What is the result of exosmosis?
Shrinkage of cell.
What are the requirements for the experiment demonstrating osmosis?
- Concentrated sugar solution
- Thistle funnel
- Cellophane paper
- Beaker containing water
State the steps in the experiment demonstrating osmosis with a thistle funnel.
- Take some concentrated sugar solution in a thistle funnel.
- Cover the mouth of the thistle funnel with a cellophane paper.
- Invert the thistle funnel in a beaker containing water and suspend it.
- Mark the level of the sugar solution on the stem of the thistle funnel.
What is the difference between the control set-up and the experimental setup in the experiment demonstrating osmosis using a thistle funnel?
The thistle funnel will have plain water instead of a concentrated sugar solution, as a control.
State the observations in the experiment demonstrating osmosis using a thistle funnel.
- After a few hours, the level of the sugar solution in the thistle funnel in the experimental setup will rise.
- Level of water in control will remain unchanged.
- In beaker in experimental setup will drop slightly while the beaker in control will remain unchanged.
- If water in beaker in experimental setup is tasted, it is not found sweet.
State the main takeaways from the thistle funnel experiment.
- In the experimental setup, some water of the beaker has passed through the cellophane paper to enter the thistle funnel containing sugar solution.
- Sugar from the thistle funnel has not passed the beaker.
- Here, the cellophane sheet acts as a selectively permeable membrane in that it allows onto water molecules from the beaker to pass into the thistle funnel and not the larger sugar molecules to paas into the beaker from the thistle funnel.
What would happen if in the experiment demonstrating osmosis, a more concentrated sugar solution would be taken in the thistle funnel and a very dilute sugar solution in the beaker?
The result would still be the same, the level of solution in the thistle funnel would rise.
Why - Some water from the dilute solution would move into the concentrated solution through the cellophane paper.
What would happen if we were to use a rubber sheet in the experiment demonstrating osmosis?
No change in the level of sugar solution would occur as the rubber sheet is impermeable and does not allow the water molecules from the beaker to cross over to the other side.
What would happen if we were to use a muslin cloth in the experiment demonstrating osmosis?
The meshes or pores of the muslim cloth are so large that they would not hold back even the sugar molecule, and the entire sugar solution would flow down to common level due to gravity.
Muslim cloth is therefore freely permeable for sugar solution.
Give alternative requirements to perform the same osmosis experiment.
- Visking bag (semi-permeable membrane)
2. Long glass capillary tube
What are the steps taken in the experiment with the visking bag?
- Place sugar solution in a knotted visking bag and insert a long glass capillary tube till some of the sugar solution rises into the capillary tube.
- Tie the mouth of the bag firmly round the capillary tube and support it on a clamp stand.
- Immerse the visking bag in a beaker with water.
- After about an hour, the level of sugar solution in the capillary tube rises.
- This rise is due to water molecules diffusing through the visking bag.
What are the key points in the above osmosis experiments?
- There are two liquids of different concentrations.
(i) Two regions of different conc. of water molecules
(ii) Two regions of diff. conc. of sugar molecules. - The two liquids are separated by the cellophane sheet which acts as a semi-permeable membrane - allows the passage of molecules selectively.
How long can osmosis continue?
Theoretically, till the concentration of water molecules is equal on both sides of the membrane.
What prevents further osmosis despite unequal concentration?
When water molecules from one medium try to force through the membrane, but the weight or pressure of the other medium holds them downwards.
Why does osmosis not continue in the thistle funnel experiment?
Because with the influx of water from the beaker, the height and weight of the column of sugar solution increase. In this state of unequal equilibrium, the water molecules from the beaker tend to force upwards through the membrane, but the weight of pressure from above holds them downwards.
What would happen if an airtight piston would be used in the thistle funnel experiment?
The level of solution would not rise at all, showing thereby that there was no entry of water.
Define osmotic pressure.
Osmotic pressure is the minimum pressure that must be exerted to prevent the passage of the pure solvent into the solution when the two are separated by a semi-permeable membrane.
Define osmotic pressure simply (1 line)
Osmotic pressure is a measure of the solution’s tendency to take in water by osmosis.
What is osmotic pressure equal to?
To the weight or pressure required to nullify osmosis.
Define tonicity.
Relative concentration of the solutions that determine the direction and extent of diffusion is called tonicity.
Name the three types of solutions based on tonicity.
- Isotonic
- Hypotonic
- Hypertonic
Define isotonic solution.
In an isotonic solution, the relative concentration of water molecules and the solute on either side of the cell membrane is the same. In such a solution, there is no net movement of water molecules across the cell membrane. No osmosis occurs.
Define hypotonic solution
In a hypotonic solution, the solution outside the cell has a lower solute concentration than the fluids inside the cell. As a result, water molecules from outside will move into the cell. (endosmosis)
Define hypertonic solution.
In a hypertonic solution, the solution outside the cell has a higher solute concentration than the fluids inside the cell. Consequently, the water molecules from the interior of the cell will move out. (exosmosis)
What happens to a cell when placed in an isotonic solution?
Cell size and shape remain unchanged
What happens to a cell when placed in a hypotonic solution?
Cell slightly enlarges or even bursts.
What happens to a cell when placed in a hypertonic solution?
Cell shrinks in size and loses its shape.
What is a turgid cell?
A cell is called turgid when it is fully distended, i.e. when it cannot withstand any further inflow of water molecules.
In the case of plant cells, which extra feature determines the behaviour of the cell when subjected to varying external fluid environments?
This feature is the rigidity of the cell wall which resists bulging and protects the delicate cellular parts inside.
What are the phenomena related to the rigidity of the cell wall?
Turgidity, plasmolysis, and flaccidity
Conversely, what does the rigidity of the cell wall determine?
It determines the behavior of the cell when subjected to varying external fluid environments.
What does a semi-permeable membrane allow but prevents?
It allows a solvent to pass through it freely but prevents the passage of the solute.
Define active transport.
Active transport is the passage of a substance (salt or ion) from its lower concentration to its higher concentration using energy from the cell through a living cell membrane.
How are the directions of active transport and diffusion different?
Active transport is in a direction opposite to that of diffusion.
Which nutrients cannot pass through the cell membrane of root cells easily?
Ions of nitrates, sulphates, potassium, zinc, manganese, etc.
What do the ions of nitrates, sulphates, potassium, zinc, manganese have in common?
They cannot pass through the cell membrane of root cells easily.