Abbrevs

Question 1

TAP

Answer 1

tandem affinity purification- uses fused proteins to make cleavage sites and c-term and n-term affinity tags, 2 wash cycles using beads (IgG, CBP)

Question 2

SSLP

Answer 2

single sequence length polymorphisms

Question 3

Three ways to do interacting protein purification

Answer 3

affinity purification, immunoprecipitation, TAP

Question 4

way to quantitatively measure a trait

Answer 4

heritability: genetic variation/ phenotypic variation ALSO norm of reaction: finds phenotypic difference of same genotype under simple environmental changes

Question 5

LOD

Answer 5

statistical score showing how likely that one marker corresponds to the phenotype

Question 6

three methods for QTL

Answer 6

backcrossing, recombinant inbred lines, collaborative cross scheme

Question 7

“bait” of Y2H

Answer 7

DBD fused to protein ORF

Question 8

“prey” of Y2H

Answer 8

TAD fused to protein ORF (library)

Question 9

Interactome detection

Answer 9

Y2H, TAP, biomolecular fluorescence complementation (split-GFP), enzymatic complementation

Question 10

Proteome detection

Answer 10

differential (2D) gel electrophoresis

Question 11

tTA

Answer 11

Tet Transcriptional Activator- Tet Off System

Question 12

rtTA

Answer 12

Reverse Tet Transcriptional Activator- Tet On System

Question 13

tetO2

Answer 13

UAS of the TetR System

Question 14

viral TAF domains

Answer 14

p65, VP64

Question 15

3 nt DBD

Answer 15

zinc finger

Question 16

1 nt DBD

Answer 16

TALE nuclease/activators

Question 17

advantages of microarray

Answer 17

cheaper, faster than RNAseq

Question 18

3 disadvantages of microarray

Answer 18

have to know splice variants before, must do biological and technical (all transcriptome), sorting to the data (clustering) to draw meaning

Question 19

variations of microarray

Answer 19

cDNA, oligonucleotide