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Phlroglucin + Nitric Acid
T. Nuclear staining is poor
Flemingβs fluid
S. Uses 1mL glacial acetic acid in its formula
Citric Acid Citrate
Y. Does NOT produce cell or tissue distortion
Chelating agents
P. Combined with metallic ions
5% Na Sulfate
V. Prevent yellow coloration from nitric acid
Formol Nitric Acid
X. 1-3 days decalcifying time
Hydrochloric acid
E. Inferior compound to Nitric Acid
Prenyiβs fluid
A.10% HNO3, 5% Chromic acid, Absolute ethyl R
Sulforons acid
W. weak decalcifying agent for minute pieces
Von ebner fluid
Z. Uses concentrated hydrochloric acid
Formic acid
U. Excellent nuclear and cytoplasmic staining
Trichloroacetic acid
H. 4-8 days decalcifying time
Formic Acid - Na citrate Solution
B. Recommended for autopsy and research speci
Ion-exhange resin
C. Remove calcium ions from formic acid containing dye
Hydrochloric Acid
E. Inferior compound to Nitric Acid
Calcium Oxalate
F. Used in measuring extent of Decalcification
4% aqueous phenol
O. Tissue softener
Post decalcification
D. Saturated Lithium Carbonate Solution
Electrophoresis
I. Uses 88% Formic Acid
Nitric Acid
J. Can be simple solution or combined with other reagents
It is very slow decalcifying agent
Neutral EDTA
The most common one and the fastest decalcifying agent so far
Nitric acid
This acid decalcifying agent use strong formaldehyde of 40%
Formol nitric acid
This decalcifying agent also softens tissues at the same time
Perenyiβs fluid
Von Ebnerβs fluid uses this weak acid?
Formic acid?
This moderate acting decalcifying agent produces better nuclear staining with less tissue distortion and safer to handle compared to HNO34 and HCl
Formic acid
This method is NOT recommended for fluids containing mineral acids such as HNO3 or HCl
Ion-exchange resin
Acid used in phloroglucine method
Nitric Acid
This decalcifying method uses 37-40% formaldehyde?
De Tomasi-Coleman Method
Environmental toxin
Chromic acid