A&P Exam 1 Flashcards
Inside of every cell is ________ charge, while the outside of the cell is _______ charged.
negative, positive
What activates a ligand gated channel?
the specific chemical/substance for that channel
What activates voltage gated channels?
anything that alters electrical charge/membrane potential such as flow of ions to action potential
What are the two structures around the nucleus and what differentiates them?
smooth and rough endoplasmic reticulum
rough has ribosomes to air in protein synthesis
What role does the golgi apparatus play in protein synthesis?
“packaging plant”: puts substances in vessicles
How many cells are in the body?
37.5 trillion
What is the barrier that protects the cell from the environment?
Cell membrane
How does the cell membrane protect the cell?
by forming a phospholipid bilayer- lipid section prevents water-soluble substances from freely moving into cell
T/F ions/glucose can move freely in and out of the cell
False, they cannot due to the lipid membrane formed
glucose can to some degree
T/F Halothane moves freely into the cell:
True, no longer used due to being difficult to manage
T/F Anesthetic gases are lipid soluble:
True, quick onset/offset
What allows water to move in/out the cell?
Aquapores (tiny): needed to allow water to move across the nonpolar cell membrane
What determines if a substance can move across the cell membrane?
Permeability coefficient
Why do ions such as Cl-, K+, and Na+ have low permeability coefficients?
need precise concentrations to maintain resting membrane potential/action potentials
What are the major intracellular ions? (cations&anions)
C: potassium (K), A: phosphates (cholesterol, phospholipids,fats)
What are the major extracellular ions? (cations&anions)
C: Sodium (Na+), A: Phosphate (P)
What is the function of the molecular gradient of ions across the cell membrane?
they assist in maintaining the - inside cell /+ outside cell
What is the function of having large cholesterol, phospholipids, and fats inside the cell?
major role in maintaining negativity inside cell to compensate for high levels of potassium
What occurs to the charges inside/outside a cell when an action potential occurs?
charges flip/flop
inside: positive
outside: negative
What causes the alteration of charge inside/outside cell during AP?
major influx of sodium causes both - influx occurs due to diffusion (passive)
What is the role of calcium on an AP?
calcium enhances intracellular positivity and enters the cell AFTER it becomes positive (voltage-gated channel)
How does calcium enter the cell during an action potential?
electrical change during AP stimulates the voltage gated channels to open, allowing an influx of calcium into the cell
What is an action potential?
most commonly the stimulation from a cell to another cell/tissue to do something
What are transport channels made of?
protein
What is the function of an ion leak channel?
leak channels allow a slow amount of ions to move freely to maintain balance of ions
What is the function of membrane proteins?
provide specificity and function to a membrane
How does a carrier protein work?
substance binds to protein, protein then flips around and moves the substance into the cell
What are the two ways to transport across the cell membrane?
Diffusion: passive
Active Transport: requires energy
What drives diffusion to occur?
concentration gradient: always moves from high to low
What type of transport must be used when moving an ion against it’s concentration gradient?
active transport
What is required to fuel active transport?
ATP (energy source)
What structures allow return to baseline following an action potential?
aquaporins, aquaglyceroporins, ion channels
O2, N, CO2, alcohols, and phenols are examples of:
lipid soluble substances (moves freely across cell membrane)
What would occur when confining the space of fluid molecules?
increased energy, increased pressure
What substances can move via aquaglycerporins?
glycerol, urea, ammonia
What determines what substances pass through an ungated ion channel?
size, shape, and charge of channel/ion
What are the two ways gated channels are regulated?
voltage or chemical/ligand
How are potassium channels selective?
the channels are lined with carbonyl oxygens that strip the water off the potassium molecules, sodiums dont reach the carbonyl oxygens and end up being too large to pass through
How are sodium channels selective?
the channel is lined with negatively charged amino acids (glutamate) which attract na, k is too large
Which channel functions in an “all or none” manner?
voltage gated channels
T/F a lipid soluble molecule can facilitate transport of inorganic ions across the lipid bilayer?
true
Would you call ionophore transport of ions simple diffusion or facilitated diffusion?
facilitated diffusion carriers
What is the difference between simple and facilitated diffusion?
simple diffusion is driven solely on concentration gradient and there is no max/ceiling of movement that can occur at a time.
facilitated diffusion still relies on a concentration gradient but requires a protein channel for the substance to move across the membrane. so # of protein channels = limiting factor/max movement
What is the Vmax?
maximum concentration that can be moved across cell membrane
What is another term for facilitated diffusion?
carrier-mediated diffusion
Rate of diffusion is limited by:
number of carrier proteins and Vmax of the carrier protein (pmol/min/mg protein)
Diffusion is driven by:
concentration gradient
How should the concentration difference be calculated for diffusion?
concentration outside - concentration inside
What are the factors that affect net diffusion?
- concentration difference
- electrical potential
- hydrostatic pressure difference
T/F energy of molecules can cause them to move AGAINST concentration gradient
true, but not super frequently
Net diffusion messures:
how many molecules were moved from high to low concentration
Other than diffusion, what else moves ions?
electrical charge: will draw opposite charge (like charges repell)
What is Nernst Potential/Equation?
theoretical intracellular electrical potential that is = in magnitude but opposite in direction to the concentration (chem) force
basically equal charge/concentration equilibrium (resting membrane)
When will the net inward current of positively charged molecules become zero?
when the electrochemical equilibrium has been achieved
What are the two things that can move a cell from the point of equilibrium?
- change in ion concentration
- change in electrical charge
What occurs when hydrostatic pressure increases?
increased pressure causes an increase in energy which is then used for movement from high to low concentration to occur
What are the two types of active transport?
Primary and Secondary
What is active transport?
movement of an ion against concentration gradient (requires energy source such as ATP)
What is used as the energy source for Primary active transport?
ATP is directly used as energy for work
How does Secondary active transport occur?
it is driven by energy stored in the electrochemical gradient of another molecule (usually Na+)
indirect used of energy
What Primary active transporter is found in every cell of every tissue of the body?
Na-K-ATPase pump
What is the movement of Na and K via Na-K-ATPase pump?
3 Na’s out, 2 K’s in
What is the deeper reason for the Na-K-ATPase pump?
It works to restore cellular resting membrane potential. (negative charge)
What occurs to K when Na rushes into the cell during an action potential?
It leaks out of the cell via leak channels… so they can be used in the Na K ATP pump
How does Digoxin work?
inhibits na k pump which results in ^ intracellular Na+ (decreased gradient)
decreased Na/Ca antiporter
intracellular Ca+2 increases
increases contractility
Muscle AP is driven by ______ not sodium.
Calcium
Apart from restoring ion concentration/gradient in cell, what else does NA K pump manage?
Osmotic Balance: moving na out decreases intracellular volume and thus, is activated by increased intracellular volume
How much energy does NA K pump require?
1/5 normal cells energy, 2/3 nervous cells energy
What are the two other types of primary active transporters?
Ca2+ATPase, H+ ATPase
What are the two active transporters present in the cell membrane of muscle fibers?
- Na K pump
- Ca2+ pump (also in the sarcoplasmic reticulum)
How does the Ca2+ ATP pump contribute to a muscle fiber?
maintains low cytosolic Ca2+ concentration
Where are the 2 locations H+ ATPase pumps found?
- Parietal gastric gland cells (HCl secretion)
- intercalated cells of the renal tubule (controls pH)
In the setting of metabolic acidosis, what occurs with the H+ ATPase pump in the kidneys?
dumps out Hydrogen ions to assist in restoring normal pH
How does the H+ ATPase pump work?
concentrated H+ ions up to 1 million-fold.
free hydrogens = acid
carbonic acid-bicarbonate buffer system uses H and HCO3 to manage/balance pH at a cellular level
What is the most important chemical process that occurs in the body?
carbonic acid-bicarbonate buffer system
H2O + CO2 <> H2CO3(carbonic acid) > H+ + HCO3
drives respiratory rate and tissue perfusion (O2/CO2)
How is most of the CO2 produced in the body returned to the lungs?
by combining with H2O to form carbonic acid then transformed into H+ + HCO3, then chemical process is reversed and CO2 and H2O is exhaled
What is required for secondary active transporters to work?
an electrochemical gradient (and stored energy)
Secondary active transporters use protein cotransporters called _______ and ________.
Antiporters & symporters
What is the difference between antiporters and symporters used in secondary active transport?
Antiporters move in opposite directions while symporters move in the same direction
How do symporters work?
They use energy from a “driver” ion such as sodium to move both na and another substance in the same direction
What transporters works by transporting substances in opposite directions, using “driver” ions like sodium?
Antiporters (one from intracellular, one from extracellular)
Where does transcellular transport of glucose and amino acids frequently occur?
Epithelial cells of GI tract
How does transcellular transport work?
Cell absorbs substance from the lumen then transports substance on the other side into a vessel or interstitial tissue/space
Osmosis is the net diffusion of:
Water
What drives the movement of water during the process of osmosis?
moves down concentration gradient (usually +2 elemenets)
Osmotic pressure can be defined as:
The pressure required to counter osmosis
(usually attributed to the osmolarity of a solution)
What stops osmotic pressure from drawing water into a solution?
it stops when a balanced state is achieved
What is tonicity?
the impact on osmolality in relation to a cell
What is an Osmole of a substance?
the molecular weight of a substance, same as a mole technically.
an osmole is the “drawing” ability of a solution
What are examples of isotonic solutions and why are they used first line?
0.9% NS and Lactated Ringer’s
there is equal tonicity between the fluids and the cells, thus will not cause an alteration in the cellular fluid/volume status which could cause harm
Which two types (tonicity) of fluids have a cellular impact?
Hypertonic (shrink cells)
Hypotonic (swell cells)
due to osmotic pressure moving water into the cell
Causes of hyper/hypo natremia:
hyponatremia: ^ h2o ingestion, SIADH
hypernatremia: excess h2o loss/sweat, central/nephrogenic DI
If you have a higher osmolality inside the cell than outside, which way will h2o move and why?
water will move into the cell in attempt to achieve an equilibrium state.
What is the difference between diffusion and osmosis?
Diffusion: can only move particles to achieve equal state
osmosis: can only move water to achieve an equal state
What are the two processes that assist the cell in returning to resting membrane potential?
Na K pump and Leak channels
In the Na and K leak channels, what prevents the ions from diffusing to the point of equilibrium?
Charge on the inside of the cell becomes “negative enough” to attract potassium and prevent it from diffusing out regardless of concentration gradient (Nernst equation)
The electrical potential that counters net diffusion of K+ is called:
K+ equilibrium potential (this refers to one element alone)
What are the Electrical potentials for both K and Na?
K: -94
Na: +61
at resting membrane state
What is Vm and why is it so close to Ek?
resting potential- because the membrane is far more permeable to k than na (100x more permeable) also 100x more leak channels
What is the difference in transporters for sodium and potassium?
Potassium transporters are lined with carbonyl oxygens while sodium transporters are lined with negatively charged amino acids (glutamates)
What is the major point regarding the Goldman-Hodgkin-Katz equation?
Resting membrane potential will always be closest to the equilibrium potential for the ion with the highest permeability. (totally independent of concentrations)
What is the resting membrane potential for skeletal muscle fibers?
-85 to 95mV
What is the resting membrane potential for smooth muscle fibers?
-50 to -60mV
What is the resting membrane potential for astrocytes?
-80 to -90mV
What is the resting membrane potential for Neurons?
-60 to -70mV
What is the resting membrane potential for Erythrocytes?
-8 to -12mV
What is the resting membrane potential for Photoreceptor cells?
-40mV (dark) to -70mV (light)
Why is the resting membrane potential for Erythrocytes so close to 0mV?
Because they are so permeable to water (and thus sodium)
What is the net drawing force of an ion?
the difference in millivolts between the membrane potential and the equilibrium potential for that ion
What is the rationale for having different net driving forces for different ions?
This allows them to move in and out of a cell at different rates due to this force which allows the cell to reach specific electrical membrane “goals”
What would occur if Na had the net driving force of K?
It wouldn’t rush into the cell once membrane potential reaches threshold as it does.
What is an overshoot of an action potential?
Means to become “too positive” above 0mV, occurs because Na gates dont always close soon enough
What occurs when we reach “threshold” in a cell?
The inside becomes more positive
What is another term for being at resting potential?
Polarized
What is the term used for the phase that occurs between resting (polarized) state to threshold?
Depolarization (move more positive)
What is the ion movement that causes depolarization?
Na movement into the cell
Why can slight stimulus occur without yielding an action potential?
They do not reach “threshold” state (not depolarized enough to cause an action potential)
What is threshold typically in relation to resting?
15-20mV less or more positive
What occurs to the na gates at threshold?
All voltage gated Na channels are instantly wide open, allowing Na to rush in and depolarize the cell
What causes Na channels to close after an AP occurs?
0mV charge closes the voltage gated channels
At 0mV, when Na gates close, what else occurs?
K gates open, potassium flows out, causing cell to become more negative which leads to repolarization
What 2 things cause repolarization after an action potential occurs?
- K gates open, potassium moves out
- Na K pumps- 3 Na out, 2 K in
Hyperpolarization is the opposite of:
Overshoot
What causes hyperpolarization?
resting membrane becomes too negative due to voltage gates closing slightly after the trigger at -90mV
What fixes hyperpolarization and restores resting membrane potential?
K leak channels (more of these- greater impact)
Na K Pump (removes Na from inside/restores balance)
Repolarization brings cell membrane:
back to resting potential
What must be reached for an action potential to occur?
MUST REACH THRESHOLD
What are the effects of depolarization on a cell?
Excitation
What are the effects of hyperkalemia on threshold potential and cell excitability?
hyperkalemia increases positive charge inside and outside the cell this brings the cell’s resting membrane much closer to threshold which causes cells to be much more excitable (smaller stimulus needed)
What is the effect of low plasma calcium on the threshold potential?
threshold becomes more negative and thus closer to Vm. This increases the excitability of the cell.
What role does calcium play in resting membrane potential?
Calcium impacts na pump function (less positive charges outside cell) more negative outside which is closer to normal membrane potential
What two electrolyte alterations can cause cells to be hypersensitive to action potentials?
Hyperkalemia (increased membrane potential)
Hypocalcemia (decreased threshold)
Why do you give calcium in the setting of hyperkalemia?
to drive potassium back into the cell and increase threshold to prevent hyperexcitability
^ K takes Vm to -80mV, -75mV is threshold = arrhythmias. giving calcium increases threshold from -75mV to -65mV to widen that gap between resting and threshold to prevent ectopy
How does low sodium in blood cause muscle tetany?
low calcium increases excitability of nerve axons by causing sodium channels to open following very small increases in Vm
The regenerating depolarization of membrane potential that propagates along an excitable membrane is defined as:
an action potential
An action potential cannot occur unless:
Membrane potential reaches threshold (depolarizes- all or nothing)
Calcium affects ______, while sodium and potassium affect _______ ________.
Threshold, membrane potential (ca also affects how sodium exits cell)
How long does it typically take for an AP to occur?
1 millisecond
What are the 5 basics of action potentials?
- all-or-nothing (to reach threshold)
- constant amplitude (do not summate- frequency not amplitude)
- initiation by depolarization
- involve changes of permeability
- rely on volt. gated channels (na in skeletal, ca in cardiac)
________ potential is closer to the _______ potential that is more _________ in the cell membrane.
resting | equilibrium | permeable
What can stop an action potential from occurring once it reaches threshold?
absolutely nothing
How is the process of a cardiac action potential different than skeletal muscle?
It is primarily driven by calcium rather than sodium, calcium channels close much slower which causes a “plateau” phase (refractory)
Is conduction velocity of an action potential constant or varying?
Conduction is constant, this is true for any given fiber
What factors alter conduction velocity of an action potential?
myelination/demyelination
diameter of fibers/type of fibers (sensory quality)
What type of fibers are better conductors?
myelinated fibers
What type of fibers conduct pain sensations?
unmyelinated C Fibers
_____ permeability increases during the upstroke of an action potential.
Sodium, this assists in allowing a depolarization to occur by attracting sodium when all the gates open- thus resulting in a greater impact
What initiates the increase of sodium permeability during the upstroke of an AP?
stimulus such as a voltage change or impulse
During the upstroke of an AP is the membrane potential closer to Ek or Ena?
Ena due to the increased permeability to sodium during that phase.
What occurs to membrane permeability during the downstroke of an action potential?
na permeability decreases, k permeability increases due to opening of k channels
During the downstroke of an AP is the membrane potential closer to Ek or Ena?
Ek due to increased k permeability
What is the difference between Absolute refractory period and Relative refractory period?
impossible for AP to occur during absolute due to voltage inactivation of Na channels, can occur during relative but must have a lot of stimulation
What is the purpose of having a refractory period?
refractory periods limit the maximum frequency of APs