99m-Tc RP QC P III

Question 1

Solid-Phase extraction

Answer 1

• RCP test
• pre-packaged cartridges
• Disposible
• may be reused
• Use solvents of different polarities to separate different chemical species

Question 2

Sep-pak QC procedure

Answer 2

1. Sample Preparation (0.1 ml of RP in tuberculin syringe)
2. Condition Sep-Pak ® (Wet/Activate)
   ▪ 1-2 mL Ethanol, Methanol, HCL, Acetonitrile, Saline or Sterile Water
   (solvent)
3. Add 0.1 ml of RP sample to the long end (inlet) of the Sep-Pak ®
4. Rinse Sep-Pak ®
   ▪ Ethanol , Methanol, HCL, saline (depending on RP)
   ▪ Collect each in a separate falcon tube (Fractions) – Bound, Free, HR
   ▪ Push 10 ml of air through the Sep-Pak ® after final rinse
5. Assay components (Falcon tubes & Sep-Pak ® individually)
6. Calculate % Radiochemical Purity according to Package Insert

Question 3

Considerations for sep-pak QC

Answer 3

• accuracy vaires with flow rate and sample load
• Flow rate: 15-20ml/min
• FR too high- no interaction
• Backsplash- contaminations and falsely low RCP
• RP loaded in wrong end
• wrong sep-pak used

Question 4

RCP limits for radiopharmaceuticals

Answer 4

• HMPAO: >/= 80%
• DMSA: >/=85%
• Sulfur colloid: >/= 92%
• others >/= 90%