9: Regulation of Acid-Base Balance Flashcards

1
Q

Explain how/why the acid-base status affects proteins

A

Acid-base status is determined by the cc of H+ ions
Proteins contain titratable groups –> reversibly bind H+ ions –> deprotonate in response extracellular pH changes
–> changes the local charge density –> affecting proteins’ shape and biochemical behavior

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2
Q

Name 4 processes in the body that affect the plasma hydrogen levels

A

1) metabolism of ingested food
2) secretions of the GI tract
3) de novo generation of acids and bases from metabolism of stored fat and glycogen
4) changes in production and exhalation of CO2

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3
Q

What is a conjugate base?

A

the particular base formed when a given acid dissociates into a proton and a base

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4
Q

What is the isohydric principle?

A

acid and bases in solution are tied to one another beause they are exposed to and bind to the same hydrogen ion concentration

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5
Q

What are the most important buffer systems?

A
  • phosphate
  • proteins - albumin and hemoglobin
  • CO2-bicarbonate buffer system
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6
Q

what are “fixed acids”?

A

fixed acids are non-volatile (e.g., acetic acid, lactic acid)
CO2 is a volatile acid - can evaporate

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7
Q

What is the conjugate base of CO2?

A

bicarbonate

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8
Q

Explain how these two equations differ

A

The first equation shows the classic Henderson-Hasselbalch equation without carbonic anhydrase but with the step of carbonic acid

the left part the equation would be very slow –> most tissues have carbonic anhydrase –> speeds it up and skips step of carbonic acid

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9
Q

Where are the chemoreceptors located that detect CO2 concentration?

A
  • brain stem - central
  • arterial - peripheral
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10
Q

How do the pancreatic and liver secretions affect the duodenal pH?

A

secrete large amounts of bicarbonate –> alkalinize

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11
Q

Compare the effects of metabolism of proteins, carbohydrates, and fats on the acid/base status

A

Proteins/Aminoacids
* depending on the protein/AA they can have alkalinizing or acidifcying effects
* e.g. sulfur-containing AA and ones with cationic side chains –> metabolized to CO2, H2O, urea –> end-result fixed acid, i.e., sulfuric acid –> quickly buffered to sulfate
* e.g., phosphorylated proteins –> phosphoric acid buffered to HPO42-
* e.g., amino acids with anionic side chains —> oxidative metabolism –> add base –> consumes H+

Carbohydrates and fat –> acid-base neutral under normal conditions

  • anaerobic metabolism of carbohydrates –> lactic acid (fixed acid)
  • metabolism of triglycerides to beta-hydroxybutyrate and acetoacetate –> ketone bodies (fixed aids
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12
Q

Explain why the lactate in LRS does not cause a lactic acidosis

A

LRS - contains the conjugated base of lactic acid -> will be oxidised to CO2 + H2O while taking up hydrogen ions + helps produce bicarbonate –> alkalizing/buffering

during lactic acidosis (e.g., circulatory shock), the body produces equal amounts lactate and hydrogen ions –> acidosis

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13
Q

where is most bicarbonate absorbed (name percentage as well)

A

proximal tubule - 80-90%

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14
Q

Describe the process of proximal tubular bicarbonate reabsorption

A
  • basolater Na/K/ATPase pump –> decrease IC Na cc
  • luminal Na/H - antiporter –> Na absorption IC and H+ excretion to lumen
  • hydrogen ion reacts with bicarbonate (exallerated by carbonic anhydrase on the luminal cell surface) –> CO2 and H2O
  • diffuses into cell via aquaporin 1 (AQP1) and through lipid bilayer
  • CO2 in cell bind with water –> CA –> bicarbonate and H+
  • H+ exits cell again via H+/ATPase and NaH-antiporter
  • formed HCO3- –> leaves cell via Na-3HCO3 symporter
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15
Q

How much and by what mechanism is bicarbonate excreted in the loop of henle?

A

~ 10%
NHE3 antiporter

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16
Q

What are the two mechanisms by which bicarbonate can exit the tubular cells on the basolateral side?

A

depending on the location within the tubule:
* Cl-HCO3 antiporter
* Na-3HCO3 symporter

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17
Q

What tubular segment is responsible for responding to acid or base loads?

A

distal segments, specifically the collecting ducts

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18
Q

What distal collecting duct cells are responsible for increased bicarbonate excretion and for increased acid secretion?

A

type A intercalated cells –> acid secretion
type B intercalated cells –> base secretion

19
Q

Explain how type B intercalated cells secrete bicarbonate

A
  • Cl-HCO3- antiporter is located on the luminal instead of basolateral membrane
  • H2O + CO2 in cell –> HCO3- + H+
  • HCO3- secreted on liminal membrane
  • H+ reabsorbed on basolateral membre via H+/ATPase
  • Cl channel on basolateral membrane facilitating Cl movement
20
Q

Explain how type A intercalated cells respond to an acid load

A

don’t just reabsorb/generate new HCO3-, but also secrete H+ that then binds to secreted bases to form undissociated acids to be urinated out

  • IC CA forms H+ and HCO3-
  • HCO3- moves out of cell on basolateral side via Cl-HCO3- antiporter (also called AE1)
  • H+ moves out of cell on luminal side via (1) H-ATPase and (2) H-K-ATPases
21
Q

What is the lowest urine pH that can be achieved with free H+ excretion?

A

pH 4.4

22
Q
A
23
Q

Name the most important filtered and synthesized bases

A

phosphate: filtered
ammonia: synthesized

24
Q

what are the acid and base forms of phosphate

A

monovalent dihydrogen phosphate - weak acid = H2PO4-
divalent monohydrgen phosphate - conjugate base = HPO42-

25
Q

what are the proportions of monovalent and divalent phosphate in the blood under normal pH conditions?

A

monovalent - 20% (acid)
divalent - 80% (base)

26
Q

What is the fraction of phosphate reabsorbed in the kidneys?

A

75-90%

27
Q

How is P transported in the blood?

A

90% free
10% losely bound to plasma proteins

28
Q

How does phosphate facilitate renal acid secretion?

A

filtered divalent monohydrogen phosphate (HPO42-) –> binds free H+ in distal nephron –> enables excretion of H+ via monovalent dihydrogen phosphate

29
Q

What mechanism of H+ excretion is quantitatively more important, ammoniagenesis or phosphate?

A

ammoniagenesis

30
Q

Explain the steps of the metabolism of protein to urea

A

protein –> broken down into AA –>
* AA carboxyl group –> bicarbonate
* AA amino group –> ammonium (NH4+), i.e., protonated form of ammonia
* ammonium + bicarbonate –> urea or glutamine

i.e., the produced bicarbonate is used up immediately for the next step

31
Q

What is the difference between ammonium and ammonia and at physiologic pH, what are their proportions?

A

ammonium - protonated, weak acid (NH4+) = 98%
ammonia - conjugate base (NH3) = 2%

32
Q

How do the kidneys handle glutamine?

A
  • freely filtred
  • taken up by the proximal tubules from (1) lumen and (2) from the interstitium via Na-glutamine symporters
  • glutamine is then IC –> cells convert glutamine back to bicarbonate and NH4+
  • NH4+ secreted into the lumen
  • bicarbonate exits into the interstitium –> blood
33
Q

Explain how the kidneys limit luminal ammonia/ammonium cc in the cortex?

A

The ammonium secreted into the proximal tubular lumen –> 80% is reabsorbed in the thick ascending loop of Henle via the Na-K-2Cl multiporter (Ammonium substituting for K) and then exiting into tinerstitium via Na-ammonium antiporter
–> net result = accumulation of ammonium in the medullary interstitium

this interstitial ammonium is again secreted in the medullary collecting ducts
* ammonium taken up from interstitium via Rh glycoprotein uniporters + some via Na-K-ATPase (ammonium substitiuting for K)
* ammonia secreted into the lumen via apical Rh glycoprotein
* combines with H+ in lumen (H+ was secreted via H-ATPase) –> ammonium

34
Q

How is Ammonium (NH4+) secreted in the proximal tubules?

A

NHE3 antiporter - ammonium is substituting for H+ and exchanging for Na

35
Q

How do you quantify the excretion of acid/base in the urine?

A

(1) amount of titratable acidity
(2) amount of ammonium
(3) amount of bicarbonate

net acid excretion = titratable acid excreted + NH4+ excreted - HCO3- excreted

36
Q

What are the different names for type A and type B intercalated cell Cl-HCO3- antiporters?

A

type A: AE1
type B: pendrin

37
Q

How do the kidneys respond to an acid load?

A

increases the number of apical H-ATPases and basolateral Cl-HCO3- (AE1)
increases ammonia production

38
Q

How do the kidneys respond to a base load?

A
  • shifts Cl-HCO3- antiporters (pendrin) to the apical membrane (to excrete more bicarbonate)
  • shifts H-ATPase to the basolateral membrane
  • decreases ammonia production
39
Q

Does hyperaldosteronism cause acidosis or alkalosis?

A

alkalosis

40
Q

How does acidosis affect the body’s glutamine handling?

A

acidosis –> liver will use more ammonium to form glutamine instead of urea –> more glutamine available for the kidneys
–> more glutamine uptake by the proximal tubular cells for oxidation and bicarbonate production

process takes a few days

41
Q

What are the 4 types of renal tubular acidosis?

A

Type 1: classical distal RTA
Type 2: proximal RTA
Type 3: combination of types 1 and 2 (rare)
Type 4: hyperkalemic RTA

42
Q

What is the mechanism behind proximal renal tubular acidosis?

A

generalized proximal tubular transport failure, i.e., Fanconi syndrome
* transport of almost all substances in the proximal tubules are impaired
* excretion of large bicarbonate amounts
* urine is acidic due to the distal hydrogen ion secretion
* plasma bicarbonate stabilizes typically around 15 mEq/L
* typically associated with hypokalemia because the large amounts of unreabsorbed solutes in the distal nephron stimulates K secretion

43
Q

What is the mechanism behind classical distal renal tubular acidosis?

A
  • defect in acid secretion by the type A intercalated cells –> defects in either the basolateral H-Cl antiporter AE1 or the apical H-ATPase
  • usually associated with hypokalemia
44
Q

What is the mechanism leading to hyperkalemic RTA?

A

hypoaldosteronism –> decreases potassium secretion and leads to hyperkalemia
hyperkalemia –> reduces ability to take up glutamine or synthesize ammonium