8_8_16 Amino Acids and Protein Structure

Question 1

2 main classes of protein

Answer 1

structural and dynamic

Question 2

Protein structure (primary)

Answer 2

sequence of amino acids going from an amine terminus to a carboxy terminus

Question 3

Amino Acids (general)

Answer 3

Zwitterionic, pKa of N-term -9, C-term -2, all L configuration (CORN)

Question 4

potential a.a. modifications

Answer 4

phosphorylation: Ser, Thr, Tyr
O-linked glycosylation: Ser, Thr
N-linked glycosylation: Asn
Disulfide bond: Cys

Question 5

Henderson-Hasselbach equation

Answer 5

pH = pKa +log([A-]/[HA])

Question 6

Alpha helix

Answer 6

right handed, dipole, H-bonds (n to n+4), 3.6 residues/turn, 5/4Å/turn, 1.5Å/residue, side chains point out and down at 100˚ intervals

Question 7

beta-sheets

Answer 7

3 perpendicular dir., 3.5Å/residue, H-bonds between strands, side chains go up and down, antiparallel are non-polar to polar etc.
parallel is all nonpolar