8.5 Gram-Positive and Gram-Negative Cocci Flashcards by Marcus Cooper

The test used most often to separate Staphylococcus and Micrococcus spp. from Streptococcus spp. is:
A. Bacitracin
B. Catalase
C. Hemolysis pattern
D. All of these options

B. Catalase

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Micrococcus and Staphylococcus spp. are differentiated by which test(s)?
A. Fermentation of glucose (OF tube)
B. Catalase test
C. Gram stain
D. All of these options

A. Fermentation of glucose (OF tube)

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Lysostaphin is used to differentiate Staphylococcus from which other gram-positive, catalase-positive organisms?
A. Streptococcus
B. Aerococcus
C. Micrococcus
D. Enterococcus

C. Micrococcus

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Which of the following tests is used routinely to identify S. aureus?
A. Slide coagulase test
B. Tube coagulase test
C. Latex agglutination
D. All of these options

D. All of these options

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Which of the following enzymes contribute to the virulence of S. aureus?
A. Urease and lecithinase
B. Hyaluronidase and β-lactamase
C. Lecithinase and catalase
D. Cytochrome oxidase

B. Hyaluronidase and β-lactamase

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Toxic shock syndrome is attributed to infection with:
A. Staphylococcus epidermidis
B. Staphylococcus hominis
C. Staphylococcus aureus
D. Staphylococcus saprophyticus

C. Staphylococcus aureus

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Which Staphylococcus species, in addition to S. aureus, also produces coagulase using the tube coagulase test?
A. Staphylococcus intermedius
B. Staphylococcus saprophyticus
C. Staphylococcus hominis
D. Staphylococcus haemolyticus

A. Staphylococcus intermedius

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S. epidermidis (coagulase negative) is recovered from which of the following sources?
A. Prosthetic heart valves
B. Intravenous catheters
C. Urinary tract
D. All of the above

D. All of the above

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Slime production is associated with which Staphylococcus species?
A. Staphylococcus aureus
B. Staphylococcus epidermidis
C. Staphylococcus intermedius
D. Staphylococcus saprophyticus

B. Staphylococcus epidermidis

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Strains of Staphylococcus species resistant to the β-lactam antibiotics by standardized disk diffusion and broth microdilution susceptibility methods are called:
A. Heteroresistant
B. Bacteriophage group 52A
C. Cross-resistant
D. Plasmid-altered

A. Heteroresistant

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S. saprophyticus is best differentiated from S. epidermidis by its resistance to:
A. 5 μg of lysostaphin
B. 5 μg of novobiocin
C. 10 units of penicillin
D. 0.04 unit of bacitracin

B. 5 μg of novobiocin

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The following results were observed by using a tube coagulase test:
Coagulase at 4 hours = + Coagulase at 18 hours = Neg
DNase = + Novobiocin = Sensitive (16-mm zone)
Hemolysis on blood agar = β Mannitol salt plate = + (acid production)
What is the most probable identification?
A. Staphylococcus saprophyticus
B. Staphylococcus epidermidis
C. Staphylococcus aureus
D. Staphylococcus hominis

C. Staphylococcus aureus

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S. aureus recovered from a wound culture gave the following antibiotic sensitivity pattern by the standardized Kirby-Bauer method (S = sensitive; R = resistant):
Penicillin = R
Cephalothin = R
Vancomycin = S
Ampicillin = S
Cefoxitin = R
Methicillin = R
Which is the drug of choice for treating this infection?
A. Penicillin
B. Ampicillin
C. Cephalothin
D. Vancomycin

D. Vancomycin

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Which of the following tests should be used to following a coagulase-positive tube test (free coagulase) to separate S. aureus from S. intermedius? The culture specimen was obtained from a patient who suffered a dog-bite wound.
A. Acetoin
B. Catalase
C. Slide coagulase
D. Urease

A. Acetoin

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A gram-positive coccus recovered from a wound ulcer from a 31-year-old patient with diabetes showed pale yellow, creamy, β-hemolytic colonies on blood agar. Given the following test results, what is the most likely identification?
Catalase = +
Glucose OF: Positive open tube, negative sealed tube
Mannitol salt = Neg
Slide coagulase = Neg
A. Staphylococcus aureus
B. Staphylococcus epidermidis
C. Micrococcus spp.
D. Streptococcus spp.

C. Micrococcus spp.

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Urine cultured from the catheter of an 18-year-old female patient produced more than 100,000 col/mL on a CNA plate. Colonies were catalase positive, coagulase negative by the latex agglutination slide method as well as the tube coagulase test. The best single test for identification is:
A. Lactose fermentation
B. Urease
C. Catalase
D. Novobiocin susceptibility

D. Novobiocin susceptibility

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A Staphylococcus spp. recovered from a wound (cellulitis) was negative for the slide coagulase test (clumping factor) and negative for novobiocin resistance. The next test(s) needed for identification is (are):
A. Tube coagulase test
B. β-Hemolysis on blood agar
C. Mannitol salt agar plate
D. All of these options

D. All of these options

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Furazolidone (Furoxone) susceptibility is a test used to differentiate:
A. Staphylococcus spp. from Micrococcus spp.
B. Streptococcus spp. from Staphylococcus spp.
C. Staphylococcus spp. from Pseudomonas spp.
D. Streptococcus spp. from Micrococcus spp.

A. Staphylococcus spp. from Micrococcus spp.

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Bacitracin resistance (0.04 unit) is used to differentiate:
A. Micrococcus spp. from Staphylococcus spp.
B. Staphylococcus spp. from Neisseria spp.
C. Planococcus spp. from Micrococcus spp.
D. Staphylococcus spp. from Streptococcus spp.

A. Micrococcus spp. from Staphylococcus spp.

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Which of the following tests will rapidly differentiate micrococci from staphylococci?
A. Catalase
B. Coagulase
C. Modified oxidase
D. Novobiocin susceptibility

C. Modified oxidase

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Streptococcus species exhibit which of the following properties?
A. Aerobic, oxidase positive, and catalase positive
B. Facultative anaerobic, oxidase negative, catalase negative
C. Facultative anaerobic, β-hemolytic, catalase positive
D. May be α-, β-, or γ-hemolytic, catalase positive

B. Facultative anaerobic, oxidase negative, catalase negative

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Which group of β-hemolytic streptococci is associated with erythrogenic toxin production?
A. Group A
B. Group B
C. Group C
D. Group G

A. Group A

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A fourfold rise in titer of which antibody is the best indicator of a recent infection with group A β-hemolytic streptococci?
A. Anti-streptolysin O
B. Anti-streptolysin S
C. Anti-A
D. Anti-B

A. Anti-streptolysin O

The L-pyrrolidonyl-β-napthylamide (PYR), hippurate, and CAMP tests can be used for the presumptive identification of which group of β-hemolytic streptococci?
A. Groups A and B
B. Groups C and D
C. Group C
D. Group F

A. Groups A and B

To diagnose streptococcal pharyngitis, primary plates of blood agar and blood agar with sulfamethoxazole-trimethoprim (SXT) are used to differentiate which streptococci? A. α-Hemolytic streptococci B. β-Hemolytic streptococci C. Streptococcus pneumoniae D. Enterococcus faecalis

B. β-Hemolytic streptococci

β-Hemolytic streptococci, not of group A or B, usually exhibit which of the following reactions? Bacitracin Sulfamethoxazole-Trimethoprim A. Susceptible Resistant B. Resistant Resistant C. Resistant Susceptible D. Susceptible or resistant Susceptible

D. Susceptible or resistant Susceptible

A false-positive CAMP test result for the presumptive identification of group B streptococci may occur if the plate is incubated in a(n): A. Candle jar or CO2 incubator B. Ambient air incubator C. 35°C incubator D. 37°C incubator

A. Candle jar or CO2 incubator

Which test is used to differentiate α-hemolytic streptococci from the Enterococcus spp. And S. bovis growing on blood agar? A. Bacitracin disk test B. CAMP test C. Hippurate hydrolysis test D. Bile esculin test

D. Bile esculin test

The bile solubility test causes the lysis of: A. Streptococcus bovis colonies on a blood agar plate B. Streptococcus pneumoniae colonies on a blood agar plate C. Group A streptococci in broth culture D. Group B streptococci in broth culture

B. Streptococcus pneumoniae colonies on a blood agar plate

S. pneumoniae and the α-hemolytic viridans streptococci can be differentiated by which test? A. Optochin P disk test, 5 μg/mL or less B. Bacitracin A disk test, 0.04 unit C. CAMP test D. Bile esculin test

A. Optochin P disk test, 5 μg/mL or less

The salt tolerance test (6.5% salt broth) is used to presumptively identify: A. Streptococcus pneumoniae B. Streptococcus bovis C. Viridans streptococci D. Enterococcus faecalis

D. Enterococcus faecalis

In addition to E. faecalis, which other streptococci will grow in 6.5% salt broth? A. Group A streptococci B. Group B streptococci C. Streptococcus pneumoniae D. Streptococcus bovis

B. Group B streptococci

A spinal fluid specimen and blood cultures were obtained from a 1-year-old infant with a respiratory infection. Growth on blood agar showed nonhemolytic, streptococci. The Quellung test is used to identify which of the following Streptococcus species? A. Streptococcus pyogenes B. Streptococcus agalactiae C. Viridans streptococci D. Streptococcus pneumoniae

D. Streptococcus pneumoniae

The PYR hydrolysis test is a presumptive test for which group of β-hemolytic streptococci? A. Group A and Enterococcus B. Group A and B β-hemolytic streptococci C. Non–group A or non–group B β-hemolytic streptococci D. Streptococcus pneumoniae and Streptococcus bovis

A. Group A and Enterococcus

A pure culture of β-hemolytic streptococci recovered from a leg wound ulcer gave the following reactions: CAMP test = Neg Bile esculin = Neg Hippurate hydrolysis = Neg 6.5% salt = Neg PYR = Neg Optochin = Resistant Bacitracin = Resistant SXT = Sensitive The most likely identification is: A. Group A streptococci B. Group B streptococci C. Enterococcus spp. D. Non–group A, non–group B streptococci

D. Non–group A, non–group B streptococci

β-Hemolytic streptococci, greater than 50,000 col/mL, were isolated from a urinary tract catheter urine specimen. Given the following reactions, what is the most likely identification? CAMP test = Neg Bile solubility = Neg PYR = + SXT = Resistant Optochin = Resistant Hippurate hydrolysis = ± 6.5% salt = + Bile esculin = + Bacitracin = Resistant Vancomycin = Resistant A. Group A streptococci B. Group B streptococci C. Enterococcus faecalis, Enterococcus faecium D. Non–group A, non–group B streptococci

C. Enterococcus faecalis, Enterococcus faecium

Abiotrophia spp. or nutritionally variant streptococci (NVS) require specific thiol compounds, cysteine, or the active form (pyridoxal) of vitamin B6. Which of the following tests supplies these requirements for growth? A. CAMP test B. Bacitracin susceptibility test C. Bile solubility test D. Staphylococcal cross-streak test

D. Staphylococcal cross-streak test

Many β-hemolytic streptococci recovered from a wound were found to be penicillin resistant. Given the following results, what is the most likely identification? Bile esculin = + PYR = + 6.5% salt = + Hippurate hydrolysis = + SXT = Resistant Bile solubility = Neg A. Enterococcus faecalis B. Streptococcus pneumoniae C. Streptococcus bovis D. Group B streptococci

A. Enterococcus faecalis

Which two tests best differentiate α-hemolytic S. bovis from Streptococcus salivarius which is also α-hemolytic? A. Bile esculin and 6.5% salt broth B. Starch hydrolysis and acid production from mannitol C. Bacitracin and PYR D. SXT susceptibility and PYR

B. Starch hydrolysis and acid production from mannitol

Two blood cultures obtained from a newborn grew β-hemolytic streptococci with the following reactions: CAMP test = + Bile solubility = Neg Bacitracin = Resistant Hippurate hydrolysis = + 6.5% salt = + Bile esculin = Neg PYR = Neg SXT = Resistant Which is the most likely identification? A. Group A streptococci B. Group B streptococci C. Enterococcus faecalis D. Non–group A, non–group B streptococci

B. Group B streptococci

MTM medium is used primarily for the selective recovery of which pathogenic Neisseria spp. organism from genital specimens? A. Neisseria gonorrhoeae B. Neisseria lactamica C. Neisseria sicca D. Neisseria flavescens

A. Neisseria gonorrhoeae

Variation in colony types seen with fresh isolates of N. gonorrhoeae and sometimes with N. meningitidis are the result of: A. Multiple nutritional requirements B. Pili on the cell surface C. Use of a transparent medium D. All of these options

D. All of these options

Gram-negative diplococci recovered from an MTM plate and giving a positive oxidase test result can be presumptively identified as: A. Neisseria gonorrhoeae B. Neisseria meningitidis C. Neisseria lactamica D. All of these options

D. All of these options

Colonies growing on MTM and showing a 4+ reaction with the Superoxol test may be considered as a rapid presumptive test for: A. Neisseria gonorrhoeae B. Neisseria meningitidis C. Neisseria lactamica D. Moraxella (Branhamella) catarrhalis

A. Neisseria gonorrhoeae

Nonpathogenic Moraxella spp. capable of growing on selective media for Neisseria can best be differentiated from pathogenic Neisseria spp. by which test? A. Catalase test B. 10-unit penicillin disk on blood agar C. Oxidase test D. Superoxol test

B. 10-unit penicillin disk on blood agar

A Gram stain of a urethral discharge from a man showing extracellular and intracellular gram-negative diplococci within segmented neutrophils is a presumptive identification for: A. Neisseria gonorrhoeae B. Neisseria meningitidis C. Moraxella (Branhamella) catarrhalis D. Neisseria lactamica

A. Neisseria gonorrhoeae

The β-galactosidase test aids in the identification of which Neisseria species? A. Neisseria lactamica B. Neisseria meningitidis C. Neisseria gonorrhoeae D. Neisseria flavescens

A. Neisseria lactamica

Cystine tryptic digest (CTA) media used for identification of Neisseria spp. should be inoculated and cultured in: A. A CO2 incubator at 35°C for 24 hours B. A CO2 incubator at 42°C for up to 72 hours C. A non-CO2 incubator at 35°C for up to 72 hours D. An anaerobic incubator at 35°C for up to 72 hours

C. A non-CO2 incubator at 35°C for up to 72 hours

Culture on MTM media of a vaginal swab produced several colonies of gram-negative diplococci that were catalase and oxidase positive and Superoxol negative. Given the following carbohydrate reactions, select the most likely identification. Glucose = + Sucrose = Neg Lactose = + Maltose = + Fructose = Neg A. Neisseria gonorrhoeae B. Neisseria sicca C. Neisseria flavescens D. Neisseria lactamica

D. Neisseria lactamica

Sputum from a patient with pneumonia produced many colonies of gram-negative diplococci on a chocolate plate that were also present in fewer numbers on MTM after 48 hours. Given the following results, what is the most likely identification? Catalase = + DNase = + Glucose = Neg Lactose = Neg Fructose = Neg Oxidase = + Tributyrin hydrolysis = + Sucrose = Neg Maltose = Neg A. Moraxella catarrhalis B. Neisseria flavescens C. Neisseria sicca D. Neisseria elongata

A. Moraxella catarrhalis

Showing resistance to which drug categorizes a strain of S. aureus as MRSA? A. Oxacillin B. Colistin C. Sulfamethoxazole-Trimethoprim D. Tetracycline

A. Oxacillin

An oxacillin-disk screen test is used to detect S. pneumoniae resistance to penicillin. Using Mueller-Hinton agar with 5% sheep blood and a 1-μg oxacillin disk, what is the recommended inhibition zone size for penicillin susceptibility? A. 5 mm or greater B. 10 mm or greater C. 15 mm or greater D. 20 mm or greater

D. 20 mm or greater

Which one of the following organisms is a known producer of β-lactamase–producing strains, and should be tested (screened) by a commercial β-lactamase assay prior to susceptibility testing? A. Streptococcus pneumoniae B. Group B streptococci C. Enterococcus spp. D. Planococcus spp.

C. Enterococcus spp.

Which test is used for the determination of inducible clindamycin resistance in staphylococci and streptococci? A. E-test B. D-zone test C. A-test D. CAMP test

B. D-zone test

A wound specimen was obtained from an older patient with diabetes and identified as being positive for vancomycin-resistant S. aureus (VRSA). The method used to determine VRSA was the standard microdilution technique. To be considered VSRA positive, which MIC must have been reported? A. MIC 16μg/mL or greater B. MIC of 8 to 16 μg/mL C. MIC of 4 to 8 μg/mL D. MIC 8 μg/mL or greater

A. MIC 16μg/mL or greater

“Scalded skin syndrome” was a suspected infection obtained from a skin culture from a newborn. Gram-positive, catalase-positive white colonies that were β-hemolytic grew on blood agar. What is the most likely identification? A. Streptococcus pyogenes B. Staphylococcus aureus C. Enterococcus faecalis D. Streptococcus agalactiae

B. Staphylococcus aureus