8-12 Flashcards
CONSIDERED TO BE THE UNIVERSAL RBC DONORS
TYPE O
WHY IS TYPE O THE UNIVERSAL DNORS
due to the absence of A and B antigen on their Red cells
genes are autosomal codominant located on the short arm of what chromosome
chromosome 1
Rh antigens are inherited as
codominant alleles
Rh antigens are transmembrane polypeptides and are integral part of
RBC membrane
frequency of D
85 %
frequency of d
15%
frequency of c
80 %
frequency of C
70%
frequency of E
30 %
frequency of e
98%
DCE terminology is also known as
fisher-race
Rh-Hr terminology is also known as
weiner
Controlled by 3 closed linked loci
Rh inheritance
each locus has its own alleles which are
Dd,Cc,Ee
theory of multiple allelomorphoric gene
weiner
ALPHA/numeric terminology terminology is also known as
rosenfield
this system has no genetic basis
rosenfield
it simply demonstrate the presence or absence of the antigen in the red blood cell
rosenfield
minujs sign preceding a number designates
absence of antigen
numeric terminology
ISBT
UNIVERSAL LANGUAGE
ISBT
ADOPTED A ——– FOR EACH AUTHENTICATED BLOOD GRP SPECIFICITY
6-DIGIT
2ND 3 NUMBERS
ANTIGEN SPECIFICITY
1ST 3 NUMBERS
SYSTEM
IgG in nature reacts at 37 degrees in AHG
rh antibodies
do not bind complement
rh antibodies
exposure to ——— of rh positive red cells can stimulate antibody production in an rh negative px
< 1 ml
refers to the presence or absence of the red cell D
Rh + , Rh -
most important red cell antigen in transfusion practice
D atigen
wht does D ANTIGEN has than other red cell antigen
greater immunogenicity
what isformed in transfusion and pregnancy
formation of anti-D
routine pre transfusion studies
only anti-D
other reagents
resolution of antibody problems or in family studies
methods used in experiment 9
slide method
tube method
reagents used in exp 9
anti-D antiserum
sample used in experiment 9 for slide mtd
capillary blood
sample used in experiment 9 for tube mtd
2-5 % rcs
surface temperature
45-50 degrees celsius
mins in rh viewbox
5 mins
agglutination in the D tube combined with the smooth suspension in control tube red cell are
D +/ Rh +
result when there is a smooth suspension of red cells in both anti D and the control tube
negative
test performed to differentiate weak D expression from rh negative phenotype
antihuman globulin test (indirect)
