7. Transport in plants Flashcards

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1
Q

Adhesion (water movement)

A

The formation of hydrogen bonds between carbohydrates in
the xylem vessel walls and water molecules. This contributes to the capillarity of water and transpiration pull.

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2
Q

Apoplastic pathway

A

One of two pathways by which water and minerals move across the root. Water moves through intercellular spaces between cellulose molecules in the cell wall.

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3
Q

Casparian strip

A

A waterproof strip surrounding the endodermal cells of the root that blocks the apoplast pathway, forcing water through the symplast route.

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3
Q

Cohesion (water movement)

A

The formation of hydrogen bonds between water molecules. This contributes to the capillarity of water and transpiration pull.

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4
Q

Cohesion-tension theory

A

The model that explains the movement of water from the soil to the leaves in a continuous stream.

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5
Q

Companion cells

A

Active cells of the phloem located adjacent to the sieve tube elements which produce ATP for metabolic processes in both themselves and the sieve tube elements. They retain their nucleus and organelles.

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6
Q

Cotransport

A

A form of secondary active transport. The movement of one substance down its concentration or electrochemical gradient is coupled to the transport of another substance via transmembrane proteins.

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7
Q

Dicotyledonous plants

A

Plants that produce seeds that contain two cotyledons. They have two primary leaves.

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8
Q

Endodermis

A

The innermost layer of the cortex of a dicotyledon root. It is impregnated with suberin which forms the Casparian strip. Endodermal cells actively transport mineral ions into the xylem.

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9
Q

Evaporation

A

A transition from the liquid state to the gaseous state which requires heat energy.

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10
Q

Eyepiece graticule

A

A scale bar inside the eyepiece of a light microscope which can be calibrated against a ruler to measure structures.

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11
Q

Herbaceous plants

A

Plants which do not have woody stems.

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12
Q

Hydrostatic pressure

A

The force which fluid molecules exert on the walls of a vessel.

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13
Q

Lignin

A

An organic polymer that has a role in the support and impermeability of vascular tissues.

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14
Q

Mass flow

A

Sugars flow passively from the source to the sink down a hydrostatic pressure gradient.

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15
Q

Phloem

A

A living plant transport vessel responsible for the transfer of assimilates to all parts of the plant. The phloem consists of sieve tube elements and companion cells.

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16
Q

Potometer

A

An apparatus used to measure water uptake from a cut shoot.

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17
Q

Root hair cells

A

Specialised cells responsible for the uptake of water and minerals from the soil. They have long hair-like extensions known as root hairs, which are adapted as exchange surfaces.

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18
Q

Sieve plates

A

The perforated end walls of sieve tube elements that allow plant assimilates to flow between cells unimpeded.

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19
Q

Sieve tube elements

A

The main cells of the phloem. They are elongated cells laid end-to-end with sieve plates between. They contain few organelles.

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20
Q

Sinks (plants)

A

The regions of a plant that remove assimilates e.g. roots, meristem, fruits.

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21
Q

Sources (plants)

A

The regions of a plant that produce assimilates e.g. leaves, storage organs.

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22
Q

Suberin

A

A waterproof, waxy material that forms the Casparian strip in the endodermis.

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23
Q

Symplastic pathway

A

One of two pathways by which water and minerals move across the root. Water enters the cytoplasm through the plasma membrane and moves between adjacent cells via plasmodesmata. Water diffuses down its water potential gradient by osmosis.

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24
Q

Translocation

A

The bulk movement of organic compounds in plants from sources to sinks via the phloem.

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25
Q

Transpiration

A

Water loss from plant leaves and stems via diffusion and evaporation. The rate of transpiration is affected by light, temperature, humidity, air movement and soil-water availability.

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26
Q

Water potential

A

A measure of the tendency of water molecules to move from one area to another measured in kilopascals (kPa) and given the symbol .

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27
Q

Xerophytes

A

Plants that are adapted to live and reproduce in dry habitats where water availability is low, e.g. cacti and marram grass.

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28
Q

Xylem

A

A non-living, heavily lignified plant transport vessel responsible for the transfer of water and minerals from the roots to the shoots and leaves.

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29
Q

Why do plants need a transport system (instead of using diffusion and osmosis)?

A
  1. Transportation of assimilates from source to sink.
  2. Transport of water and mineral ions across far distances e.g., the roots to leaves of a very tall tree.
  3. Plant cannot be supported by only short-distance transport methods (osmosis and diffusion). Would take too long for water, mineral ions and assimilates to reach destinations. Therefore, not efficient enough.
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30
Q

Assimilates

A

organic products synthesised as a result of photosynthesis.

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31
Q

What are the features of plant transport systems?

A
  1. Require vascular tissue made of xylem and phloem.
    * Xylem – transports water and mineral ions from roots to stems and leaves.
    * Phloem – transports assimilates from source to sink.
  2. Long distance bulk transport systems. (Mass flow theory).
  3. Transport tissue distributed between roots, stem and leaves so that all cells can receive their required mineral ions and assimilates.
  4. A transport medium in which to transport dissolved substances. (Xylem or phloem sap).
  5. Movement of transport medium using pressure differences.
  6. Gaseous exchange does not occur in transport tissues (unlike mammals).
  7. Transport system is unidirectional, as in, it is not a circular flow (like mammal circulatory system). However, the phloem can transport assimilates both up and down.
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32
Q

Herbaceous plants

A

plants that have a soft and non-woody stem, ALSO, plants that have a life cycle of only one year.

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33
Q

The difference between dicotyledonous and monocotyledonous plants?

A

Dicotyledonous plants – flowering plants developed from seeds that have two embryonic leaves/cotyledons. Their leaves are in multiples of 4 or 5 and have a reticulated vein network. In stems, vascular tissues arranged in bundles in a ring pattern.
VS
Monocotyledonous plants – herbaceous flowering plants developed from seed with only one embryonic leaf. Leaves are in multiples of 3 and have parallel veins. In stems, vascular tissues is arranges in bundles scattered around stem.

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34
Q

Why should plants be structured to maximise SA:V?

A

Plant leaves and root systems need to maximise their SA:V as much as possible in order to facilitate the uptake of water and mineral ions, as well as the absorption of sunlight and carbon dioxide.

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35
Q

How do herbaceous vs woody plants facilitate gaseous exchange differently?

A

In herbaceous plants, the stems (and leaves) contain stomata which facilitate gaseous exchange.
In woody plants, there are lenticels to facilitate gas exchange.

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36
Q

How are the stem and roots structured?

A

Stem and roots are both organs containing different tissues. Above soil, plant is structured to withstand different mechanical stresses, such as wind. Plants are supported by sclerenchyma, parenchyma and collenchyma tissues. Vascular bundle arrangement differs to perform particular function.

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37
Q

Epidermis

A

outermost layer of stem and roots. Protective layer of cells that is covered by wax cuticle.

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38
Q

Describe structure and function of parenchyma tissues?

A

living undifferentiated cells in stem and roots functioning as packing tissue (fill empty spaces) AND a storage tissue.
* Spherical cells.
* Thin cellulose cell walls.
* Large vacuoles.
* Some have chloroplasts.
* Can be found in cortex of stem sand roots and pith of stems.
* Turgid parenchyma cells provide hydrostatic support.

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39
Q

Describe structure and function of collenchyma tissue?

A

living tissue specialised for support and found commonly as a layer directly beneath epidermis.
* Cellulose cell walls with uneven thickening.
* Cell walls also contain pectin.
* Adapted for areas of growth as cell walls able to stretch.

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40
Q

Describe structure and function of sclerenchyma tissues?

A

dead cells compose tissue; specialised for support and strength in areas that are no longer elongating.
* Two distinct groups: sclereids and fibres.
* Cellulose walls very thick, some walls contain lignin.
* Fibres are long.
* Sclereids are shorter and of varying shapes.

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41
Q

How is vascular tissue distributed in a dicotyledonous stem?

A
  • Vascular bundles close to outside of stem.
  • Lateral forces acting on plant are best resisted by a cylindrical non-continuous circle arrangement of vascular bundle (especially for herbaceous stems).
  • Because the stem is non-continuous the stem is still flexible.
  • Xylem inside, phloem outside.
  • Between xylem and phloem is cambium.
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42
Q

Cambium

A

meristematic tissue containing stem cells for division that can specialise into either xylem or phloem cells.

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43
Q

How is vascular tissue distributed in a dicotyledonous root?

A
  • Situated centrally because roots are subject to pulling forces.
  • Central column of supporting tissue.
  • Xylem arranged in star (4 point) shaped block of tissue surrounded by separate phloem groups.
  • Around both is the pericycle and endodermis.
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44
Q

How is the structure of root hair cells adapted to their function?

A
  • Epidermis in area with plentiful root hair cells known as piliferous layer.
  • Root hair cell is a modified epidermal cell.
  • Cell has a large vacuole.
  • Long cytoplasmic extension projecting.
  • Large surface area for water and mineral uptake.
  • Root hair delicate - does not live for long.
  • As root grows, new root hair cells nearer to tip replace old ones.
  • No waxy cuticle.
  • Thin cellulose cell wall.
  • Many aquaporins (channel proteins for water uptake) in cell surface membrane.
  • Many mitochondria to provide ATP for active uptake.
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45
Q

What to include in plan diagram?

A
  • Individual cells not drawn.
  • Cells not drawn but tissues labelled.
  • Shows proportions of different tissues.
  • Annotations should be added if required, and include description of shape, size and colour. (E.g., differences in staining).
  • Lines should be clear and continuous.
  • Make proportions shown realistic.
  • Use all available space.
  • Do not shade or colour.
  • Label lines should not have arrowheads or be crossed.
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46
Q

How is vascular tissue distributed in a leaf?

A
  • Vascular tissue is situated in reticulated vein network in lamina (blade of leaf) form side veins.
  • Side veins merge into central main vein or midrib.
  • Xylem on top, phloem on the underside.
  • Veins often surrounded by ring of parenchyma cells.
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47
Q

Node

A

Point where leaf is attached to stem.

48
Q

Petiole

A

Leaf stalk

49
Q

How is the leaf structured (overview of components)?

A
  • Epidermis
  • Palisade mesophyll
  • Spongy mesophyll
  • Phloem
  • Xylem
  • Parenchyma, sclerenchyma, collenchyma
50
Q

Structure and function of epidermis?

A
  • Colourless cells that allow light to enter the leaf.
  • Protects cell.
  • Specialised epidermal cells = guard cells.
  • Guard cells enclose stomata.
  • Gaseous exchange takes place through stomata.
  • Water vapour evaporates in spongy mesophyll but is LOST through stomata via diffusion.
  • Outer surface of epidermis covered by waxy waterproof cuticle to limit water loss.
51
Q

Which colours will lignin and cellulose stain in observation of plant specimens?

A
  • Lignin – stains pink (e.g., xylem and sclerenchyma)
  • Cellulose – stains blue or green.
52
Q

Structure and function of palisade mesophyll tissue?

A
  • Adapted to carry out photosynthesis.
  • Main tissue that carries out photosynthesis.
  • Closely packed and thin-walled so absorbs maximum light.
  • Arranged vertically so fewer cross walls to filter out light.
  • Numerous chloroplasts that are arranged in best positions to absorb light.
  • Large vacuole pushes cytoplasm and chloroplasts to edge of cells – absorb light and SHORT diffusion distance for CO2.
  • Large surface area and moist, thin walls for rapid diffusion of gases.
53
Q

Structure and function of spongy mesophyll tissue?

A
  • Loosely packed to create large intercellular air spaces.
  • Air spaces facilitate evaporation, whereby water vapour then diffuses out from stomata.
  • Spongy mesophyll can photosynthesise and store starch.
54
Q

Equation for photosynthesis?

A

6CO2 + 6H2O = C6H12O6 + 6O2

55
Q

Function of xylem?

A
  • Main water conducting tissue in vascular plants.
  • Transports xylem sap (watery solution of dissolved minerals, plant hormones and other nutrients).
  • Provides hydrostatic and structural support for plants.
56
Q

Why are xylem elements termed ‘elements’ and not cells?

A

Cell is a living structure but mature xylem vessels are dead.

57
Q

How are xylem vessel elements structured?

A
  • Vary in structure.
  • All hollow and elongated.
  • As they mature walls become impregnated with lignin.
  • Lignin causes cell to die because it is unable to obtain water.
  • End walls broken down – forms continuous tube known as xylem vessel.
  • Non-lignified areas termed pits – not completely open, still retain cellulose cell wall.
  • Cell walls contain cellulose within lignin.
58
Q

What do xylem pits allow for?

A

Lateral movement of water.

59
Q

In what two ways does lignin thicken the xylem vessel? Which is better?

A
  • Annular thickening (forms rings).
  • Reticulate thickening (forms a spiral or network).
  • Reticulate thickening better because allows for elongation of vessels as plant grows.
60
Q

Rules for drawing xylem vessel elements?

A
  • If high powered drawing: do NOT include xylem parenchyma or sclerenchyma fibres.
  • Make sure walls appear thick (use eyepiece graticule to give correct proportion of wall to lumen).
  • Lumen has no contents and can be annotated as hollow.
  • Pits too small to be seen.
  • No cell surface membrane.
61
Q

What are xylem fibres?

A

Elongated sclerenchyma cells with walls that are thickened with lignin to provide support.

62
Q

What is lignin?

A

A biological polymer that provides mechanical strength.

63
Q

What is secondary thickening?

A

When cellulose cell wall is thickened with lignin.

64
Q

How does structure of xylem vessel elements relate to function?

A
  • Continuous elongated cells = unobstructed flow.
  • Vessel is hollow and dead = no cytoplasmic contents to obstruct flow.
  • Secondary thickening = can withstand tension; hydrophobic so waterproofs.
  • Annular, spiral and reticulate lignin thickening = mechanical support; allow xylem vessel to elongate during growth.
  • Pits = lateral flow of water. Can connect adjacent xylem vessels. Water can use pits to detour around air bubbles obstructing flow.
  • Cellulose walls = adhesion. Helps water resist gravity and maintain upward flow.
65
Q

Describe xylem parenchyma?

A
  • Unspecialised cells act as packing tissues.
  • Roughly spherical in shape but when TURGID press upon and flatten each other.
  • Therefore, provide hydrostatic support.
66
Q

Describe tracheids?

A
  • Other cell type in xylem tissue.
  • Basically the same as xylem vessels.
  • Longer, thinner, tapering ends.
  • Thickened with lignin.
  • Pits to allow lateral movement of water.
67
Q

Phloem sap

A

mineral ions, sugars, amino acids, plant hormones dissolved in water

68
Q

What is the structure of the phloem (overview)?

A
  • Sieve tube elements
  • Companion cells
69
Q

Structure of sieve tube elements:

A
  • Elongated cells form long tube.
  • Peripheral cytoplasm found under cell surface membrane. Mitochondria and modified form of endoplasmic reticulum can be found. No nucleus, no ribosomes, no Golgi body. These are present in companion cells so fewer cellular structures obstruct flow of phloem sap.
  • NB: sieve tube cells are living, unlike mature xylem vessels.
  • End walls of sieve tubes perforated (pierced with holes) by large pores (2-6 um in diameter). These are termed sieve plates.
  • Central space in sieve tube is termed the lumen.
70
Q

What is the relation between companion cells and phloem sieve tube elements?

A
  • Comes from same cell division as corresponding phloem sieve tube element.
  • Companion cells carry out life processes necessary to sustain phloem sieve tub elements and itself. (Somewhat of a surrogate cell).
71
Q

What is the structure of a companion cell?

A
  • Many plasmodesmata for loading of assimilates.
  • Specific membrane carrier proteins for areas lacking plasmodesmata.
  • Many organelles, dense cytoplasm, thin cellulose cell wall.
72
Q

What are transfer cells?

A
  • Specialised companion cells found in leaf tips.
  • Very folded cell walls and cell surface membranes. (increases SA:V)
  • Large SA increases rate of transfer of sucrose into sieve tube elements.
73
Q

How does the structure of phloem sieve tube elements relate to their function?

A
  • Elongated and arranged to form continuous tube.
  • Missing many organelles for unobstructed flow, open lumen.
  • Sieve plates perforated, reducing resistance to flow.
  • Sieve plates hold walls of sieve tube elements together – prevents lysis from pressure.
  • Individual cell walls contain cellulose microfibrils, provides strength.
74
Q

How does the structure of companion cells relate to their function?

A
  • Contain organelles to physiologically support sieve tube.
  • Many mitochondria to release ATP needed for translocation.
  • Plasmodesmata in regions of loading and unloading.
75
Q

How to identify phloem sieve tube elements in a prepared slide?

A
  • Phloem sieve tube elements have thinner walls proportionate to their lumen than xylem vessel elements.
  • Cell wall contains cellulose so should stain blue/green.
  • Longitudinally appear as elongated individual cells joined by cross walls (sieve plates).
76
Q

What colour will xylem vessel wall stain in a prepared slide?

A

Red. (Lignin stains pink.)

77
Q

How to identify companion cells in a prepared slide?

A
  • Longitudinally appear much narrower than phloem sieve tube element.
  • Smaller diameter.
78
Q

How to draw phloem sieve tube elements and companion cells from a prepared slide?

A

Draw cells touching each other unless specified otherwise.

79
Q

What is the outlined pathway of water from root to leaf?

A

Root hair cells take up water -> water passes through root cortex into xylem -> xylem carries water up to aerial parts.

80
Q

How does water get taken up by root hair cells?

A
  • Root hairs extend into spaces around soil particles.
  • Surrounded by soil solution (mostly water and little bit of mineral ions).
  • Soil solution has higher W.P than root hair cell so moves via osmosis into cell.
  • From root hair cell water moves across cortex through either apoplast pathway or symplast pathway.
81
Q

How does the apoplast pathway work?

A
  • Basically: water is transported through the cell walls of cortical cells.
  • Water (starts off in root hair cell walls) -> root cortex -> endodermis (ring around xylem and phloem).
  • As water is drawn into endodermal cells, PULLS more water along with it. Due to COHESION.
  • This causes a tension that draws water along cell walls of cortical cells and along intercellular spaces between cortical cells.
  • Moves from cell to cell in this manner.
82
Q

Why can apoplast pathway be described as non-living?

A

Water does not cross any cell surface membranes or enter cytoplasm of any cells.

83
Q

How does the symplast pathway work?

A
  • Basically: water moves across cytoplasm of cortical cells as a result of W.P differences between cells.
  • Water moves from cell to cell through PLASMODESMATA so does NOT cross through cell wall or membrane.
  • Water enters root hair cell and makes W.P higher.
  • That root hair cell then has higher W.P than adjacent cortical cell.
  • Water moved from root hair cell into cortex via plasmodesmata (down W.P gradient).
  • Water moves towards core of cortical from cell to cell down W.P gradient (like dominoes).
  • AT SAME TIME, W.P in first cortical cell is lowered as water moves towards cortex so more water is drawn up to replaces it. Goes on and on and on: (water potential gradient is established).

Note: Symplast pathway is slower because flow is obstructed by cytoplasm and organelles.

84
Q

Protoplast

A

all contents of cell excluding cell wall.

85
Q

How does water move from apoplast/symplast pathway into xylem?

A

Note: xylem situated in centre of root, surrounded by phloem.
* Water from apoplast pathway prevented from moving further along cell wall. Casparian strip in endodermal cells (made of waterproof suberin) stops it. *Remember endodermis is ring around xylem and phloem in root.
* Water from apoplast must join protoplast via osmosis and join up with symplast flow in cytoplasm.
* Water leaving endodermis passes through pericycle (layer of parenchyma cells).
* Once it enters the xylem it defaults back to apoplastic pathway. (so just takes a little detour with symplast pathway through endodermis).

86
Q

How do mineral ions move into the root?

A
  • Move into root mostly via active transport because very low concentration in soil compared to root hair cell.
  • Specific membrane proteins facilitate this.
87
Q

Transpiration

A

the loss of water vapour from the aerial parts of a plant

88
Q

How does water actually leave stomata?

A

Note: the main force that actually pulls water up the stem is the evaporation of water vapour from spongy mesophyll cells into intercellular air spaces, and then water diffuses out of open stomata into atmosphere.

89
Q

How does water move across the leaf?

A
  • A water potential gradient is established that pulls water from xylem, across leaf mesophyll, then into the atmosphere.
  • Humidity of atmosphere is usually less and has lower water potential than sub-stomatal air space so water vapour diffuses out of air spaces. Water vapour lost from air spaces replaced by water vapour evaporating from spongy mesophyll cells. Means that intercellular air spaces are saturated.
  • Water evaporating from cell walls of spongy mesophyll replaced by water coming from xylem via apoplast or symplast pathway.
90
Q

How can plants control water loss?

A

Plant control water loss by closing stomatal pored with guard cells.

91
Q

How does water move up stem in xylem?

A

Moves up by mechanism of cohesion-tension theory.

92
Q

How does cohesion-tension theory work?

A
  • Water evaporates from spongy mesophyll cells due to heat from the sun. Leads to transpiration.
  • Water molecules stick together by cohesion due to hydrogen bonds.
  • Water forms continuous, unbroken column across mesophyll cells and down xylem. Known as transpiration stream.
  • As water evaporates from mesophyll cells in leaf into intercellular and then sub-stomatal air spaces, more molecules of water drawn up behind as a result of cohesion.
  • Column of water pulled up from xylem as result of transpiration – known as transpiration pull.
  • Transpiration pull puts xylem under tension.
93
Q

Tension vs pressure?

A

Note: Tension = negative inwards pressure, positive pressure pushes outwards

94
Q

What is evidence for cohesion-tension theory?

A
  1. Change in tree trunk diameter
    * During height of day when transpiration is highest, there is most tension in xylem. Pulling inwards – so diameter shrinks. Lignin prevents collapse inwards of xylem.
    * When transpiration is at lowest then not as much pressure – tree trunk relaxes.
  2. Water does not leak out if xylem vessel is broken
    * Air is pulled in instead because pulling pressure not pushing pressure.
95
Q

Reasons for adhesion of water to xylem vessel?

A
  • Cellulose lining of xylem cell wall is hydrophilic.
  • Lignin (hydrophobic molecule) has hydrophilic groups.
96
Q

Xerophytes

A

plants structurally and physiologically adapted to living in areas where water losses due to transcription may be higher than their water uptake.
Without xerophytic adaptations, plants would become plasmolysed and desiccated.
When a plant cell becomes plasmolysed the cytoplasm pulls away from the cell wall.

97
Q

Cuticular evaporation

A

when water is lost from the cuticle of a plant.

98
Q

Xerophytic adaptations of leaves that reduce transpiration?

A
  • Having a thick cuticle – prevents cuticular evaporation
  • Curling up of leaves – traps a region of still air within the curled leaf, region becomes saturated with water vapour so there is only a very slight water potential gradient between sub-stomatal air space and atmosphere. Transpiration reduced considerably.
  • Having stomata in pits or grooves – trap moist air next to lead, reduce water potential gradient.
  • Having sunken stomata – stomata located just below surface of epidermis. Not directly exposed to air movements.
  • Having needle-like leaves – surface area to volume ratio reduced. Reduction in surface area balanced against need for sufficient area for photosynthesis.
  • Having trichomes (hairs) on leaves – thick layer of hair on lower epidermis traps moist air next to the leaf surface. Water potential gradient reduced.
  • Closing stomata when transpiration rates are very high – e.g., C4 plants (split light dependent and light independent phase of photosynthesis, makes efficient use of CO2) or production of plant hormone abscisic acid in response to stress and dehydration. Causes stomata to close.
  • Having a multi-layered epidermis – reduce cuticular transpiration to increase diffusion distance of water vapour from intercellular air spaces.
  • Epidermal cells and hypodermal cells may be impregnated with cutin.
99
Q

What do all these different adaptations all aim collectively to do?

A
  • Decrease transpiration rate and water loss by reducing the water potential gradient between the sub-stomatal air space and the external atmosphere.
  • Increasing diffusion distance of water vapour from saturated intercellular air spaces.
  • Maintaining volume of leaf reducing SA exposed for transpiration.
100
Q

What purposes does transpiration serve?

A
  • Supplies leaf cells with water and mineral ions
  • Supply water as a reactant
  • Maintains turgor pressure in cells for structural support
  • Supply water for phloem sap, thereby enabling translocation
101
Q

Note: Evaporation of water requires heat energy and so has a cooling effect on the plant

A

.

102
Q

Hypodermis

A

one or more layers of cells between the epidermis and palisade mesophyll.

103
Q

Cutin

A

waxy, water-repellent substance in the cuticle of plants.

104
Q

How to make annotated drawings of transverse sections of leaves from xerophytic plants?

A
  • Cuticle separate, continuous transparent (not individual cells).
  • Trichomes shown in proportion to dimensions of epidermal cells, should not be a single line.
    If you are asked to annotate drawing with notes explain in terms of:
  • Reducing water potential gradient between saturated sub-stomatal air spaces and the external atmosphere e.g., trichomes, sunken stomata, stomata in grooves or pits, curled or rolled leaves.
  • Increasing distance for diffusion of water vapour from saturated intercellular air spaces e.g., thick walled cells at epidermis or below epidermis, cuticle, hypodermis.
  • Maintaining volume of leaf but reducing SA exposed for water vapour, e.g., needle-shaped leaves.
105
Q

What are the different habitats of xerophytic plants?

A
  • Desert plants
  • Sand dunes (rainfall drains away through pores in sand)
  • Dry, windy places
  • Salt marshes
  • Cold regions (water freezes) *Note: may have curled or rolled leaves that limits transpiration but at the same time minimises SA for photosynthesis. This is because in cold regions enzymes can’t work as optimally (due to temperature) so photosynthesis takes place at a slower rate and there is a reduced need for the SA.
106
Q

Halophytes

A

plants adapted to live in saline habitats like salt marshes

107
Q

How does plant use glucose synthesised from photosynthesis?

A

Synthesis of…
* starch
* cellulose
* amino acids
* plants hormones

108
Q

sources

A

sites of synthesis

109
Q

sinks

A

sites in plant requiring assimilates

110
Q

Examples of sinks?

A

Highly metabolic areas such as:
* Buds
* Shoot tips
* Root tips
* Flowers

111
Q

Note: translocation can occur in either direction of phloem and at any one time phloem sap can be moving in different directions in different sieve tubes.

A

.

112
Q

What is in phloem sap?

A
  • Assimilates (sucrose, amino acids)
  • Plant hormones
  • Inorganic ions (potassium, chloride, phosphate)
  • Water
113
Q

Mass flow theory

A

explains mechanism of how translocation of assimilates is achieved. Consists of three phases, namely:
1. Transfer of sucrose into sieve tube elements from photosynthesising tissue
2. Movement of phloem sap in sieve tube elements from source to sink
3. Transfer of sucrose from sieve tube elements into storage or other sink cells

114
Q

Explain first phase of mass flow theory (Transfer of sucrose into sieve tube elements from photosynthesising tissue):

A

Apoplastic loading – the way that plants load sucrose into phloem sieve tubes
Process of apoplastic loading/active loading:
* Sucrose manufactured from products of photosynthesis in cells that have chloroplasts.
* Hydrogen ions/protons are actively pumped from companion cells into apoplast (cell walls and spaces between cells) using ATP.
* Hydrogen ion concentration builds up in apoplast, hydrogen ions flow back to companion cells down concentration gradient through carrier proteins (facilitated diffusion).
* Sucrose molecules transported with hydrogen molecules in process known as cotransport. Carrier proteins known as cotransporter proteins.
* In cotransport hydrogen ions are flowing back down their concentration gradient but sucrose ions are going AGAINST their concentration gradient. The slow of sucrose ions back into companion cells is powered by flow of protons.
* Sucrose molecules then move by simple diffusion through plasmodesmata from companion cell into phloem sieve tube element.

115
Q

Apoplastic loading

A

the way that plants load sucrose into phloem sieve tubes

116
Q

Explain second phase of mass flow theory (movement of phloem sap in sieve tube element from source to sink):

A

How does mass flow occur?
* Apoplastic loading causes sieve tubes to have lower W.P. than xylem. Water therefore moves from xylem to sieve tube element by osmosis – this creates a high hydrostatic pressure within them.
* At the sink, sucrose moves out of phloem sieve tubes to be used where needed.
* Increases W.P of phloem sap and water leaves phloem sieve tubes to ‘follow’ sucrose osmotically.
* Hydrostatic pressure in regions of sinks in sieve tubes is therefore low as a result of water entering sieve tubes at source.

117
Q

Mass flow

A

bulk movement of substance through given channel or area in a specified amount of time.

118
Q

Explain third phase of mass flow theory (transfer of sucrose from the sieve tube elements into storage or other sink cells):

A

During unloading of assimilates sucrose can move from sieve tube element into A) the companion cell (by diffusion) or B) into the sink cell by either diffusion or active uptake, depending on sink cell’s sucrose concentration.

Note: storage organ can be both a source and a sink. Source when energy stores have been broken down for transport into growing regions and sink when assimilates that are going to be converted into storage molecules are received.