7 | Immunochemical Techniques

Question 1

Which two compounds have substrates that produce a visable color on the blot where the antibodies have bound.?

Answer 1

peroxidase and alkaline phosphatase, However, sensitivity can be increased by the use of luminol as a substrate for peroxidase; the emitted light is detected by exposure to photographic fi lm or a charge-coupled device (CCD). The substrate reaction can be stopped after optimum colour development, and the blot dried and stored for reference.