7 - Analysis of nucleic acids Flashcards by Saira C | Brainscape

Q

What are the temperature changes in PCR?

A

90 degrees + to denature
50-60 degrees to anneal
72 degrees to extend

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Q

What are the applications of PCR?

A

detaching point mutations
cDNA cloning
gene expression (reverse transcriptase PCR)
DNA sequencing
DNA microarrays

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