Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

A-level Biology > 6C - Control of blood glucose concentration > Flashcards

6C - Control of blood glucose concentration Flashcards

You may prefer our related Brainscape-certified flashcards:
AP® Biology flashcards Biology 101 flashcards
1
Q

What is the regulation of blood glucose an example of?

A

The interactions between different hormones in achieving homeostasis.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What are the 2 types of glands?

A

Exocrine and endocrine.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What do exocrine glands do?

A

Secrete chemicals outside the body.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What do endocrine glands do?

A

Communicate using hormones, carried in circulatory system, stimulate target cells with specific receptors.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What type of response do endocrine glands produce?

A

A slow, long-lasting and widespread response.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What is the endocrine system made of?

A
  • Pituitary gland
  • Thyroid and parathyroid gland
  • Adrenal glands
  • Pancreas
  • Ovaries/uterus
  • Prostate/testes
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

What do the thyroid and parathyroid glands release?

A

Thyroxine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

What do the adrenal glands release?

A

Adrenaline

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

What do the ovaries/uterus release?

A

Oestrogen/progesterone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

What do the prostate/testes release?

A

Testosterone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What 3 main effects do hormones have in their target cells?

A
  • Affect the permeability of the cell membrane.
  • Cause the release of a ‘second messenger’ inside the cell.
  • Diffuse into the cell and promote or inhibit transcription.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Why do we need to control blood glucose?

A
  • Hypoglycemia and hyperglycemia can kill cells due to the effect on water potential of the blood and tissue fluid.
  • Required for cellular respiration.
  • Required for the manufacture of many other cellular products.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

What can change the concentration of glucose in your blood?

A

Exercise and eating.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

What is the normal concentration of glucose in the blood?

A

Around 90 mg per 100cm^3 of blood.

5 mmol dm^-3

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

What is blood glucose concentration monitored by?

A

Cells in the pancreas.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

When does blood concentration glucose rise?

A

After eating food containing carbohydrate.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

When does blood glucose concentration fall?

A

After exercise, as more glucose is used in respiration to release energy.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

What 2 hormones control blood glucose concentration?

A

Insulin and glucagon.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

What are insulin and glucagon secreted by?

A

Clusters of cells in the pancreas called islets of Langerhans.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What makes up the islets of Langerhans?

A

Alpha and beta cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What are the islets of Langerhans?

A

Group of hormone producing cells, scattered throughout the cells that make digestive enzymes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What do alpha cells do?

A

Detect low glucose concentration and secrete glucagon.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

What do alpha cells detect?

A

Low glucose concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

What do alpha cells secrete?

A

Glucagon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Q

What is the size of alpha cells like compared to beta cells?

A

Larger

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
26
Q

What do beta cells do?

A

Detect high glucose concentration and secrete insulin.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
27
Q

What do beta cells detect?

A

High glucose concentration

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
28
Q

What do beta cells secrete?

A

Insulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
29
Q

What is the size of beta cells like compared to alpha cells?

A

Smaller

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
30
Q

Which is bigger, alpha or beta cells?

A

Alpha cells are larger.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
31
Q

What type of cell secretes glucagon?

A

Alpha cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
32
Q

What type of cell secretes insulin?

A

Beta cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
33
Q

What type of cell detects low glucose concentration?

A

Alpha cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
34
Q

What type of cell detects high glucose concentration?

A

Beta cells

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
35
Q

If low glucose concentration is detected, what hormone is secreted?

A

Glucagon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
36
Q

If high glucose concentration is detected, what hormone is secreted?

A

Insulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
37
Q

From what 3 sources does blood glucose come from?

A
  • Directly from the diet.
  • Glycogen converted into glucose in the liver (glycogenolysis).
  • From gluconeogenesis.
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
38
Q

What do changes in supply and demand of glucose allow insulin, glucagon and adrenaline to do?

A

Operate to maintain a constant blood glucose concentration.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
39
Q

What do insulin and glucagon act on?

A

Effectors, which respond to restore the blood glucose concentration to the normal level.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
40
Q

What is glycogenesis?

A

Synthesis of glycogen from glucose.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
41
Q

What is glycogenolysis?

A

Breakdown of glycogen into glucose.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
42
Q

What is gluconeogenesis?

A

Synthesis of glucose from lipids, amino acids or nucleic acids.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
43
Q

What is the synthesis of glycogen from glucose called?

A

Glycogenesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
44
Q

What is the breakdown of glycogen into glucose called?

A

Glycogenolysis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
45
Q

What is the synthesis of glucose from lipids, amino acids or nucleic acids called?

A

Gluconeogenesis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
46
Q

What is glycogenesis activated by?

A

Insulin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
47
Q

What is glycogenolysis activated by?

A

Glucagon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
48
Q

What is gluconeogenesis activated by?

A

Glucagon

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
49
Q

What is another name for liver cells?

A

Hepatocytes.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
50
Q

What does insulin do?

A

Lowers blood glucose concentration when it’s too high.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
51
Q

What does glucagon do?

A

Raises blood glucose concentration when it’s too low.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
52
Q

Explain how blood glucose concentration is regulated when it is too high

A

1) Beta cells in the islets of Langerhans detect it is too high and secrete insulin into the bloodstream.
2) Insulin binds to specific receptors on the cell surface membranes of liver and muscle cells.
3) It increases the permeability of muscle-cell membranes to glucose, so the cells take up more glucose. This involves increasing the number of channel proteins in the cell membrane.
4) Insulin also activates enzymes in the liver and muscle cells that convert glucose into glycogen (glycogenesis).
5) The cells are able to store glycogen in their cytoplasm, as an energy source.
6) Insulin also increases the rate of respiration of glucose, especially in muscle cells.
7) Excess glucose may also undergo conversion to fat.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
53
Q

Where does insulin bind to lower blood glucose concentration?

A

Cell membrane of liver and muscle cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
54
Q

What does insulin do once it has bound to the cell membrane of muscle cells?

A

It increases the permeability of muscle-cell membranes to glucose, so the cells take up more glucose. This involves increasing the number of channel proteins in the cell membrane.

Insulin also activates enzymes in the liver and muscle cells that convert glucose into glycogen (glycogenesis).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
55
Q

What does insulin do once it has bound to the cell membrane of liver cells?

A

Insulin activates enzymes in the liver and muscle cells that convert glucose into glycogen (glycogenesis).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
56
Q

What does insulin do to the rate of respiration of glucose?

A

Increases the rate of respiration of glucose, especially in muscle cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
57
Q

What might happen to excess glucose when insulin tries to reduce blood glucose concentration?

A

It may undergo conversion to fat.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
58
Q

Explain how blood glucose concentration is regulated when it is too low

A

1) Alpha cells in the islets of Langerhans detect it’s low and secrete glucagon into the bloodstream.
2) Glucagon binds to specific receptors on the cell membranes of liver cells.
3) Glucagon activates enzymes in the liver cells that break down glycogen into glucose (glycogenolysis).
4) Glucagon also activates enzymes that are involved in the formation of glucose from glycerol and amino acids (gluconeogenesis).
5) Glucagon decreases the rate of respiration of glucose in cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
59
Q

Where does glucagon bind to raise blood glucose concentration?

A

Cell membranes of liver cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
60
Q

What does glucagon do once it has bound to the cell membrane of liver cells?

A
  • Activates enzymes in the liver cells that break down glycogen into glucose (glycogenolysis).
  • Activates enzymes that are involved in the formation of glucose from glycerol and amino acids (gluconeogenesis).
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
61
Q

What does glucagon do to the rate of respiration of glucose?

A

Decreases the rate of respiration of glucose in cells.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
62
Q

What feedback mechanism keeps blood glucose concentration normal?

A

Negative feedback mechanism.

63
Q

What is the response of hormone like compared to the response produced by nervous impulses and why?

A

Because hormones travel in the blood to their target cells, the response produced are slower but longer lasting (as hormones aren’t broken down as quickly as neurotransmitters).

64
Q

Explain how the negative feedback mechanism is involved when blood glucose becomes too high?

A
  • Pancreas detects blood glucose concentration is too high.
  • Beta cells secrete insulin and alpha cells stop secreting glucagon.
  • Insulin binds to receptors on liver and muscle cells.
  • Cells take up more glucose, glycogenesis is activated and cells respire more glucose.
  • Less glucose in the blood.
65
Q

Explain how the negative feedback mechanism is involved when blood glucose becomes too low?

A
  • Pancreas detects blood glucose concentration is too low.
  • Alpha cells secrete glucagon and beta cells stop secreting insulin.
  • Glucagon binds to receptors on liver cells.
  • Glycogenolysis is activated, gluconeogenesis is activated and cells respire less glucose.
  • Cells release glucose into the blood.
66
Q

What is GLUT4?

A

A glucose transporter.

67
Q

What does insulin do to glucose transporters?

A

Makes them available for facilitated diffusion.

68
Q

What do skeletal and cardiac muscle cells contain which help facilitated diffusion?

A

A channel protein called GLUT4 which is a glucose transporter.

69
Q

What happens to GLUT4 when insulin levels are low?

A

GLUT4 is stored in vesicles in the cytoplasm of cells.

70
Q

What happens to GLUT4 when insulin binds to receptors on the cell-surface membrane?

A

It triggers the movement of GLUT4 to the membrane.

71
Q

What can happen to glucose by the GLUT4 protein?

A

Glucose can be transported into the cell through the GLUT4 protein, by facilitated diffusion.

72
Q

What hormone is adrenaline like?

A

Glucagon

73
Q

What does adrenaline do to blood glucose concentration?

A

Increases it.

74
Q

Where are the adrenal glands found?

A

Just above your kidneys.

75
Q

When is adrenaline secreted?

A

When there’s a low concentration of glucose in your blood, when you’re stressed and when you’re exercising.

76
Q

Explain the interaction between insulin and glucagon in the regulation of blood glucose

A
  • Act in opposite directions (antagonistically).
  • System is self-regulating through feedback, concentration of glucose in blood that determines the quantity of hormone produced.
  • The concentration of blood glucose isn’t constant, process of negative feedback work, only when concentrations fall or increase beyond the set point.
77
Q

How does adrenaline regulate blood glucose concentration?

A
  • Increases blood glucose concentration.
  • Adrenaline binds to receptors in the cell membrane of liver cells.
  • This activates glycogenolysis and inhibits glycogenesis.
  • Also activates glucagon secretion and inhibits insulin secretion, which increases glucose concentration.
  • Adrenaline gets the body ready for action by making more glucose available for muscles to respire.
78
Q

What does adrenaline activate when regulating blood glucose concentration?

A

Glycogenolysis and glucagon secretion .

79
Q

What does adrenaline inhibit when regulating blood glucose concentration?

A

Glycogenesis and insulin secretion.

80
Q

Explain the second messenger model

A

1) Adrenaline and glucagon bind to their receptors and activate an enzyme called adenylate (adenylyl) cyclase.
2) This binding causes the protein inside the cell to change shape.
3) This activates the enzyme adenylyl cyclase to convert ATP into cyclic AMP, a second messenger.
4) cAMP binds to protein kinase A, activating it by changing its shape.
5) Protein kinase A activates a cascade (a chain of reactions) that breaks down glycogen into glucose (glycogenolysis), which then moves out of the liver cell and into the blood by facilitated diffusion using channel proteins.

81
Q

What can both adrenaline and glucagon activate?

A

Glycogenolysis inside a cell even though they bind to receptors on the outside of the cell.

82
Q

What do the receptors for adrenaline and glucagon have?

A

Specific tertiary structures that make them complementary in shape to their respective hormones.

83
Q

What enzyme do adrenaline and glucagon activate when they bind to their receptors?

A

Adenylate cyclase (adenylyl cyclase).

84
Q

What is adenylate cyclase (adenylyl cyclase)?

A

An enzyme.

85
Q

What does activated adenylate cyclase (adenylyl cyclase) do?

A

Converts ATP into a chemical signal called a ‘second messenger’ - cyclic AMP (cAMP).

86
Q

What is ATP converted into in the second messenger model?

A

A chemical signal called a ‘second messenger’ - cyclic AMP (cAMP).

87
Q

What is the second messenger in the second messenger model?

A

Cyclic AMP (cAMP).

88
Q

What does cyclic AMP (cAMP) do?

A

Activates protein kinase A.

89
Q

What is protein kinase A?

A

An enzyme.

90
Q

What is protein kinase A activated by?

A

Cyclic AMP (cAMP).

91
Q

What does protein kinase A do?

A

Activates a cascade (a chain of reactions) that breaks down glycogen into glucose (glycogenolysis), which then moves out of the liver cell and into the blood by facilitated diffusion using channel proteins.

92
Q

What is the first messenger in the second messenger model?

A

Adrenaline.

93
Q

When does diabetes occur?

A

When blood glucose concentration is not controlled properly.

94
Q

What is diabetes full name?

A

Diabetes mellitus.

95
Q

What is diabetes?

A

A condition where blood glucose concentration can’t be controlled properly.

The person is unable to metabolise carbohydrates properly, especially glucose.

96
Q

What is hyperglycemia?

A

Too high blood glucose.

97
Q

What is hypoglycemia?

A

Too low blood glucose.

98
Q

Where is the pancreas?

A

Upper abdomen, behind the stomach.

99
Q

What does the pancreas do?

A
  • Produces enzymes (protease, amylase, lipase) for digestion.
  • Produces hormone (insulin, glucagon) for regulating blood glucose concentration.
100
Q

Where is the liver?

A

Immediately below the diaphragm.

101
Q

What cells make up the liver?

A

Hepatocytes.

102
Q

What does the liver do?

A

Filters blood from the digestive tract. detoxifies chemicals and regulates blood glucose concentration through the use of hormones.

103
Q

What 3 important processes take place in the liver?

A

Glycogenesis, glycogenolysis, gluconeogenesis.

104
Q

Which type of diabetes is the most common?

A

Type II.

105
Q

What are the 2 types of diabetes?

A

Type I and type II.

106
Q

What is type I diabetes described as?

A

Insulin dependent.

107
Q

What happens in type I diabetes?

A

The immune system attacks the beta cells in the islets of Langerhans so they can’t produce any insulin.

(Autoimmune response.)

108
Q

What might type I diabetes be caused by?

A

Not known exactly - might be a genetic predisposition or triggered by a viral infection.

109
Q

When does type I diabetes usually begin?

A

In childhood.

110
Q

What is the onset of symptoms like in type I diabetes?

A

Rapid onset.

111
Q

What are the signs of type I diabetes?

A

Frequent urination, glucose in the urine, genital itching, weight loss, tiredness.

112
Q

What happens after eating in type I diabetes?

A

The blood glucose level rises and stays high - hyperglycemia. This can result in death if left untreated.

113
Q

What can hyperglycemia result in if left untreated?

A

Death

114
Q

Where does the glucose go in type I diabetes and why?

A

The kidneys can’t reabsorb all of the glucose so some of it is excreted in the urine.

115
Q

Roughly how many people have diabetes?

A

> 415 million people.

116
Q

What percentage of cases of diabetes does type Ii diabetes make up?

A

90%.

117
Q

How is type I diabetes treated?

A

Insulin therapy - regular insulin injections 2-4 times a day or the use of an insulin pump to deliver insulin continuously.

118
Q

Why does insulin therapy have to be carefully controlled?

A

Because too much insulin can produce a dangerous drop in blood glucose levels - hypoglycemia.

119
Q

What 2 ways can insulin therapy be delivered?

A

Regular insulin injections or the use of an insulin pump.

120
Q

What can’t insulin be taken by mouth?

A

Because it would be digested.

121
Q

What must the dose of insulin given in insulin therapy match?

A

The glucose intake.

122
Q

What can blood glucose be monitored by?

A

Biosensors.

123
Q

What has shown promise in potentially leading to a cure for type I diabetes?

A

Recent trials in transplanting insulin-producing cells have shown promise.

124
Q

What helps to avoid a sudden rise in glucose?

A

Eating regularly and controlling simple carbohydrate intake (intake of sugars).

125
Q

When does type II diabetes usually begin?

A

Later in life (over age 40).

126
Q

What is type II diabetes often linked with?

A

Obesity.

127
Q

When in type II diabetes more likely?

A

In people with a family history of the condition.

128
Q

Besides obesity, what are risk factors for type II diabetes?

A

Lack of exercise, age and poor diet.

129
Q

When does type II diabetes occur?

A

When the beta cells in the islets of Langerhans don’t produce enough insulin or when the body’s cells don’t respond properly to insulin.

130
Q

Why don’t the beta cells respond properly to insulin in type 2 diabetes?

A

Because the insulin (glycoprotein) receptors on their membranes don’t work properly (they are lost or lose responsiveness and so don’t take up enough glucose.

This means the blood glucose concentration is higher than normal.

131
Q

What is the onset of symptoms like in type II diabetes?

A

Develops slowly with symptoms not severe.

132
Q

How can type 2 diabetes be treated?

A

By eating a healthy, balanced diet, losing weight (if necessary) and regular exercise - regulating intake of carbohydrates in the diet.

Glucose-lowering medication (drugs that stimulate insulin production) can be taken if diet and exercise can’t control it.

Eventually, insulin injections may be needed.

133
Q

Can type I diabetes be cured?

A

No

134
Q

Can type II diabetes be cured?

A

Yes

135
Q

What has the increasingly common problem of type II diabetes been linked to in the UK?

A

Increasing levels of obesity, a move towards more unhealthy diets and low levels of physical activity.

136
Q

What can type II diabetes cause?

A

Additional health problems, including visual impairment and kidney failure.

137
Q

Is type I diabetes associated with high or low blood glucose?

A

High

138
Q

Is type II diabetes associated with high or low blood glucose?

A

High

139
Q

What are the signs of type II diabetes?

A

Similar to type I - frequent urination, glucose in the urine, genital itching, weight loss, tiredness.

140
Q

What do health advisors recommend that people do to reduce the risk of developing type II diabetes?

A
  • Eat a diet that’s low in fat, sugar and salt, with plenty of whole grains, fruit and vegetables.
  • Take regular exercise.
  • Lose weight if necessary.
141
Q

What aims to educate people in order to reduce their risk of developing conditions like type II diabetes and how?

A

Campaigns like the NHS’s Change4Life - educates people on how to have a healthier diet and lifestyle.

142
Q

What have health advisors challenged in order to combat type II diabetes and how?

A

Challenged the food industry to reduce the advertising of junk food (particularly to children), to improve the nutritional value of their products, and to use clearer labelling in products - allowing consumers to make healthier choices about what to buy.

143
Q

What have some food companies done in response to criticism and how (in terms of lowering the risk of type II diabetes)?

A

Some food companies have attempted to make their products more healthy, e.g.

  • Using sugar alternatives to sweeten food/drinks.
  • Reducing the sugar, fat and salt content of products.
144
Q

What practical technique is used to determine the concentration of a glucose solution?

A

Colorimetry

145
Q

What is the normal concentration of glucose in the urine?

A

Very low - between 0 and 0.8mM.

146
Q

What might a higher glucose concentration of 0-0.8 in the urine indicate?

A

Diabetes

147
Q

How do you determine the concentration of glucose in a ‘urine’ sample using colorimetry?

A

1) Make up several glucose solutions of different known concentrations.
2) Do a quantitative Benedict’s test (add quantitative Benedict’s reagent to sample and heat in a water bath that’s been brought to the boil) on each solution (plus a negative control of pure water). Use the same amount of Benedict’s solution in each case.
3) Use a colorimeter (with a red filter) to measure the absorbance of the Benedict’s solution remaining in each tube.
4) Use the results to make the calibration curve, showing absorbance against glucose concentration.
5) Then you can test the unknown solution, i.e. the ‘urine’ sample, in the same way as the known concentrations, and use the calibration curve to find the concentration of glucose in the sample.

148
Q

What is quantitative Benedict’s reagent/how is is different to normal Benedict’s reagent?

A

When heated with glucose, the initial blue colour is lost, but a brick-red precipitate is not produced.

149
Q

What does a colorimeter do when used to determine glucose concentration?

A

Measures the light absorbance of the solution after the quantitative Benedict’s test has been carried out.

150
Q

What would the result of the quantitative Benedict’s test and colorimetry be the higher the glucose concentration?

A

The higher the concentration of glucose, the more blue colour will be lost (i.e. the paler the solution will become), decreasing the absorbance of the solution.

151
Q

How would you make 5 serial dilutions with a dilution factor of 2 starting with an initial glucose concentration of 4mM?

A

1) Line up 5 test tubes in a rack.
2) Add 10cm^3 of the initial 4mM glucose solution to the first test tube and 5cm^3 of distilled water to the other 4 test tubes.
3) Then, using a pipette, draw 5cm^3 of the solution form the first test tube, add it to the distilled water in the second test tube and mix the solution thoroughly. You now have 10cm^3 of the solution that’s half as concentrated as the solution in the first test tube (it’s 2mM).
4) Repeat this process 3 more times to create solutions of 1mM, 0.5mM and 0.25mM.

152
Q

How do you do a quantitative Benedict’s test?

A

Ad quantitative Benedict’s reagent to a sample and heat it in a water bath that’s been brought to the boil.

153
Q

What are the axis on the calibration curve for glucose concentration?

A 
x = concentration of glucose
y = absorbance value

A-level Biology (42 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions