6.1 - Cellular Control Flashcards
What is an exon?
Coding part of a gene.
What is an intron?
Non-coding part of a gene.
Why is the term ‘junk’ DNA misleading?
Junk implies this DNA has no function – in truth we do not yet know the function.
What is a mutation?
A change to the base sequence.
Substitution: One or more bases are swapped for one another.
Deletion: One or more bases are removed.
Insertion: One or more bases are added.
How might a deletion of bases mutation affect protein functionality?
Deletion leads to frameshift. Changes amino acid sequence. Affects hydrogen/ionic/sulphur bonds. Changes tertiary structure of protein – so cannot function.
How might a mutation result in a non-functional protein?
Mutation causes a stop codon so transcription stopped prematurely. Mutation codes for a different amino acid to be produced - protein may/may not function.
When may a mutation not result in a non-functional protein?
Mutation codes for same amino acid to be produced due to the degenerate nature of the genetic code. Mutations that occur in the non-coding DNA regions – the introns will be removed by splicing & not be translated so have no effect on protein produced.
What are the causes of mutations?
Spontaneous during DNA replication. Mutagens - Chemicals that alter the DNA structure or high energy radiation.
What are the three levels that gene expression can be controlled at?
Transcriptional level, post-transcriptional level & post-translational level.
What is a promotor?
A region of DNA that initiates transcription (upstream) of a particular gene. Where RNA polymerase binds to initiate transcription.
What is a transcription factor?
A protein that binds to DNA to activate or repress transcription (turn genes on or off).
What determines whether a transcription factor can bind to DNA or not?
Shape – sometimes this can be altered by the binding of some molecules e.g. certain hormones & sugars.
Can transcription factors be controlled?
Yes – by the amount of certain molecules in an environment affecting transcription factor binding.
What are the functions of transcription factors (TF) in eukaryotic cells?
TF regulate gene expression - by either activating or inhibiting the binding of RNA polymerase to promoter region.
TF make sure that only certain genes in specific cell types are expressed.
TF play a role in cell differentiation & help to regulate the cell cycle and cell division.
What is an operon?
A group of genes that function as a single transcription unit.